Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LDJ84_RS27355 Genome accession   NZ_CP084413
Coordinates   5882790..5884919 (-) Length   709 a.a.
NCBI ID   WP_065327921.1    Uniprot ID   -
Organism   Pseudomonas sp. HS-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5877790..5889919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDJ84_RS27340 (LDJ84_27340) - 5879411..5881099 (-) 1689 WP_065327920.1 AAA family ATPase -
  LDJ84_RS27345 (LDJ84_27345) aroB 5881106..5882212 (-) 1107 WP_024764143.1 3-dehydroquinate synthase -
  LDJ84_RS27350 (LDJ84_27350) aroK 5882263..5882754 (-) 492 WP_052239087.1 shikimate kinase AroK -
  LDJ84_RS27355 (LDJ84_27355) pilQ 5882790..5884919 (-) 2130 WP_065327921.1 type IV pilus secretin PilQ Machinery gene
  LDJ84_RS27360 (LDJ84_27360) pilP 5884973..5885497 (-) 525 WP_065327922.1 type 4a pilus biogenesis lipoprotein PilP -
  LDJ84_RS27365 (LDJ84_27365) pilO 5885494..5886144 (-) 651 WP_065327923.1 type 4a pilus biogenesis protein PilO -
  LDJ84_RS27370 (LDJ84_27370) pilN 5886141..5886734 (-) 594 WP_065327924.1 type 4a pilus biogenesis protein PilN -
  LDJ84_RS27375 (LDJ84_27375) comM 5886734..5887798 (-) 1065 WP_024764148.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 75868.07 Da        Isoelectric Point: 5.4052

>NTDB_id=610996 LDJ84_RS27355 WP_065327921.1 5882790..5884919(-) (pilQ) [Pseudomonas sp. HS-18]
MNRTLSRLGISVLLAVFAPTLLAANLQSVDVAALPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLTTLVPYSTRADGSNLYVVVGGSAKNSTASVAAASTSVPITRPAAPRPYIPVGKAIR
NVDFQRGEQGEGNVVIDLSDPTVSPDIQEQGGKIRLDFPRTQLPDNLRVRLDVKDFATPVQFVNASAVGDKASITIEPTG
LYDYLVYQTDNRMTVSIKPLTQDDAEKRKKDSFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQISELAPLRRELIQVNYAKAADIAKLFNSVTGGAG
AGAQPDTRGSITVDDRTNAIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEASVDYSKSLGVRWGGAFTFGNWASWG
KDGNIGTGGTAGAGCGPFVNGCTLPTSGSNSPTPSVDLGATNATSGIGIGFVSNNAILDLQLSAMEKGGNGEVVSQPKVV
TSDKETAKILKGSEIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEVNAK
VLVNDGETIVIGGVFSNTQTKATDKVPFLGDVPVLGRLFRRDSVTDSKNELLVFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=610996 LDJ84_RS27355 WP_065327921.1 5882790..5884919(-) (pilQ) [Pseudomonas sp. HS-18]
ATGAATAGAACGCTTTCCCGCCTGGGCATTTCCGTGCTCCTGGCCGTATTTGCACCGACTCTGCTGGCTGCAAACCTGCA
GAGCGTCGACGTGGCGGCCCTGCCGGGCGACCGTGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTAGCCGCGCCGCGTG
GCTATACCATCGAACAGCCTGCGCGCATTGCATTGGACCTGCCGGGCGTGCAGAACAAGCTGGGTAGCAAGAATCGCGAG
TTGGGTGTCGGTAATGCCCGTAGTCTGACTGTGGTCGAGGCCAAAGATCGCACGCGTCTGATCGTGAACCTGACCACTCT
GGTCCCTTACAGCACCCGTGCTGACGGCTCCAACCTTTATGTCGTAGTAGGTGGTTCGGCCAAAAACTCGACGGCCAGCG
TTGCGGCGGCGTCGACATCTGTCCCGATCACGCGGCCGGCCGCACCTAGGCCGTACATTCCGGTGGGCAAGGCTATTCGT
AATGTTGATTTCCAGCGGGGCGAGCAGGGCGAAGGCAATGTCGTGATCGATCTGTCCGATCCGACCGTCAGCCCGGATAT
CCAGGAGCAGGGCGGCAAGATTCGTCTGGACTTCCCGCGTACCCAATTGCCGGACAACCTACGTGTACGCCTGGATGTGA
AGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCATCGGCAGTAGGTGATAAGGCCAGCATCACCATTGAGCCGACCGGG
CTATACGACTATTTGGTCTATCAGACCGATAACCGCATGACGGTCAGCATCAAGCCTCTGACCCAGGACGATGCGGAGAA
GCGCAAGAAGGACAGCTTCGCCTACTCCGGCGAGAAGCTGTCGCTGAACTTCCAAGACATTGATGTGCGGTCGGTGCTGC
AGCTTATTGCTGACTTCACCGACCTCAACCTGGTGGCTTCCGATACAGTGCAGGGCAATATCACCCTGCGTCTGCAGAAC
GTACCGTGGGATCAGGCTCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGTAAGGTTGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAGATCGCGGCTCGTGAGCGTCAGGAGTTGGAGGCGCAAAAGCAGATTTCCGAGCTGGCGCCGCTGC
GTCGTGAGCTGATTCAGGTGAACTATGCCAAGGCTGCCGACATTGCCAAACTGTTCAACTCTGTAACGGGGGGCGCGGGC
GCTGGTGCTCAGCCCGATACTCGCGGGTCTATCACTGTCGATGATCGCACCAATGCTATCATCGCGTACCAGACTCAGGA
TCGTCTGGACGAGCTGCGTCGGATTGTTGCTCAGCTGGATATTCCGGTGCGCCAAGTCATGATCGAAGCGCGCATTGTTG
AGGCCTCGGTGGATTACAGCAAAAGCCTGGGTGTTCGTTGGGGTGGCGCTTTCACCTTTGGTAACTGGGCCAGTTGGGGT
AAGGATGGCAATATTGGTACTGGTGGTACCGCCGGTGCTGGCTGTGGCCCATTTGTCAACGGATGTACTCTGCCGACATC
TGGAAGCAATTCCCCGACTCCATCGGTTGATCTCGGGGCAACCAATGCCACCTCTGGTATCGGTATCGGCTTCGTTTCCA
ACAACGCAATTCTCGACTTGCAACTCTCTGCGATGGAGAAGGGCGGCAACGGCGAGGTCGTTTCCCAGCCGAAAGTCGTT
ACTTCCGACAAGGAAACCGCAAAGATCCTCAAGGGCTCGGAGATTCCGTACCAGGAAGCCAGCTCCAGTGGCGCGACCTC
GGTGTCCTTCAAGGAGGCGGCACTGTCCTTGGAGGTGACCCCACAGATCACGCCGGACAACCGCATCATCATGGAAGTGA
AGGTCAACAAGGATGAGCCGGACTACGCCAATGCGCTCAATGGTGTTCCTCCGATCAAGAAGAACGAAGTGAATGCCAAG
GTGCTGGTCAATGATGGCGAGACCATCGTGATCGGTGGTGTATTCTCCAATACGCAGACTAAGGCGACCGACAAGGTTCC
GTTCCTGGGTGACGTTCCGGTGCTGGGCCGACTGTTCCGTCGGGATTCGGTAACCGACAGCAAGAACGAGCTACTGGTTT
TCCTTACCCCGCGGATCATGAATAATCAGGCCATTGCAGTCGGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(544-700)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

80.672

100

0.812
  pilQ Acinetobacter baumannii strain A118

39.73

100

0.415
  pilQ Acinetobacter baumannii D1279779

39.73

100

0.415
  comQ Acinetobacter baylyi ADP1

42.181

98.307

0.415
  pilQ Legionella pneumophila strain Lp02

39.941

96.051

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.941

96.051

0.384