Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LCH33_RS01725 Genome accession   NZ_CP084212
Coordinates   374734..376839 (+) Length   701 a.a.
NCBI ID   WP_005743117.1    Uniprot ID   A0AAJ2NEE0
Organism   Pseudomonas amygdali strain 35-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369734..381839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH33_RS01705 (LCH33_000339) pilM 371879..372943 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  LCH33_RS01710 (LCH33_000340) - 372943..373542 (+) 600 WP_004654254.1 PilN domain-containing protein -
  LCH33_RS01715 (LCH33_000341) pilO 373539..374162 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  LCH33_RS01720 (LCH33_000342) pilP 374159..374686 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  LCH33_RS01725 (LCH33_000343) pilQ 374734..376839 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  LCH33_RS01730 (LCH33_000344) aroK 376844..377362 (+) 519 WP_002551657.1 shikimate kinase AroK -
  LCH33_RS01735 (LCH33_000345) aroB 377478..378581 (+) 1104 WP_002551658.1 3-dehydroquinate synthase -
  LCH33_RS01740 (LCH33_000346) - 378592..380211 (+) 1620 WP_005778690.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75471.84 Da        Isoelectric Point: 5.0976

>NTDB_id=610102 LCH33_RS01725 WP_005743117.1 374734..376839(+) (pilQ) [Pseudomonas amygdali strain 35-1]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEAGNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=610102 LCH33_RS01725 WP_005743117.1 374734..376839(+) (pilQ) [Pseudomonas amygdali strain 35-1]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGTGAA
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATTAACCTGACCACGCT
TTCGCCCTACAGCTCCCGCGTCGACGGTAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAAACCGTTTATCCCTGCG
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGACTTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGCGACAAGGCCAGTATC
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGACACGGTTCAGGGCGGTATT
ACTCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCGGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
GACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTTGACATTCCGGTGCGTCAAGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAGGTAACACTGGCAGCAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTGCTGGACCTTGAAC
TGAGCGCGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACTGTGAGCTTCAAGGAGGCCTCGCT
GTCGCTTGAGGTAACGCCACAGATCACCCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGTGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.199

100

0.745
  comQ Acinetobacter baylyi ADP1

40.822

100

0.425
  pilQ Acinetobacter baumannii D1279779

38.255

100

0.407
  pilQ Acinetobacter baumannii strain A118

38.255

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.841

98.431

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.752

98.288

0.381