Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LCH33_RS01705 Genome accession   NZ_CP084212
Coordinates   371879..372943 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas amygdali strain 35-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 366879..377943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH33_RS01695 (LCH33_000337) - 366918..368186 (+) 1269 WP_002551650.1 malic enzyme-like NAD(P)-binding protein -
  LCH33_RS01700 (LCH33_000338) - 369228..371669 (-) 2442 WP_371911680.1 penicillin-binding protein 1A -
  LCH33_RS01705 (LCH33_000339) pilM 371879..372943 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  LCH33_RS01710 (LCH33_000340) - 372943..373542 (+) 600 WP_004654254.1 PilN domain-containing protein -
  LCH33_RS01715 (LCH33_000341) pilO 373539..374162 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  LCH33_RS01720 (LCH33_000342) pilP 374159..374686 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  LCH33_RS01725 (LCH33_000343) pilQ 374734..376839 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  LCH33_RS01730 (LCH33_000344) aroK 376844..377362 (+) 519 WP_002551657.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=610101 LCH33_RS01705 WP_002551652.1 371879..372943(+) (pilM) [Pseudomonas amygdali strain 35-1]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=610101 LCH33_RS01705 WP_002551652.1 371879..372943(+) (pilM) [Pseudomonas amygdali strain 35-1]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTTGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGCCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469