Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NML20_RS18460 Genome accession   NZ_CP101257
Coordinates   3784155..3785540 (+) Length   461 a.a.
NCBI ID   WP_254825220.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-078     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3779155..3790540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML20_RS18435 (NML20_18435) aroP 3779703..3781076 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NML20_RS18440 (NML20_18440) ampE 3781119..3781973 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NML20_RS18445 (NML20_18445) ampD 3781970..3782521 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NML20_RS18450 (NML20_18450) nadC 3782609..3783502 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NML20_RS18455 (NML20_18455) pilA 3783705..3784145 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NML20_RS18460 (NML20_18460) pilB 3784155..3785540 (+) 1386 WP_254825220.1 type II secretion system protein GspE Machinery gene
  NML20_RS18465 (NML20_18465) hofC 3785530..3786732 (+) 1203 WP_000157227.1 protein transport protein HofC -
  NML20_RS18470 (NML20_18470) guaC 3786767..3787810 (-) 1044 WP_001217342.1 GMP reductase -
  NML20_RS18475 (NML20_18475) - 3787966..3788010 (-) 45 WP_120795372.1 protein YacM -
  NML20_RS18480 (NML20_18480) coaE 3788035..3788655 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  NML20_RS18485 (NML20_18485) zapD 3788655..3789398 (+) 744 WP_001194734.1 cell division protein ZapD -
  NML20_RS18490 (NML20_18490) yacG 3789408..3789605 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NML20_RS18495 (NML20_18495) mutT 3789821..3790210 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50496.17 Da        Isoelectric Point: 6.3673

>NTDB_id=609672 NML20_RS18460 WP_254825220.1 3784155..3785540(+) (pilB) [Escherichia coli strain EC21Z-078]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLARTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIDIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=609672 NML20_RS18460 WP_254825220.1 3784155..3785540(+) (pilB) [Escherichia coli strain EC21Z-078]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAGCTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGATATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.476

82.863

0.41
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio campbellii strain DS40M4

48.062

83.948

0.403
  pilB Vibrio cholerae strain A1552

46.212

85.9

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.373

83.731

0.388
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369