Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML20_RS18455 Genome accession   NZ_CP101257
Coordinates   3783705..3784145 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain EC21Z-078     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3778705..3789145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML20_RS18435 (NML20_18435) aroP 3779703..3781076 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NML20_RS18440 (NML20_18440) ampE 3781119..3781973 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NML20_RS18445 (NML20_18445) ampD 3781970..3782521 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NML20_RS18450 (NML20_18450) nadC 3782609..3783502 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NML20_RS18455 (NML20_18455) pilA 3783705..3784145 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NML20_RS18460 (NML20_18460) pilB 3784155..3785540 (+) 1386 WP_254825220.1 type II secretion system protein GspE Machinery gene
  NML20_RS18465 (NML20_18465) hofC 3785530..3786732 (+) 1203 WP_000157227.1 protein transport protein HofC -
  NML20_RS18470 (NML20_18470) guaC 3786767..3787810 (-) 1044 WP_001217342.1 GMP reductase -
  NML20_RS18475 (NML20_18475) - 3787966..3788010 (-) 45 WP_120795372.1 protein YacM -
  NML20_RS18480 (NML20_18480) coaE 3788035..3788655 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=609671 NML20_RS18455 WP_000360895.1 3783705..3784145(+) (pilA) [Escherichia coli strain EC21Z-078]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=609671 NML20_RS18455 WP_000360895.1 3783705..3784145(+) (pilA) [Escherichia coli strain EC21Z-078]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363