Detailed information NTG0608689

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LCH16_RS12890
• Genome accession: NZ_CP083943
• Coordinates: 2661466..2662080 (-)
• Length: 204 a.a.
• NCBI ID: WP_061860722.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CSUSB3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2656466..2667080

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCH16_RS12860 (LCH16_12860)	rpsT	2656769..2657035 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LCH16_RS12865 (LCH16_12865)	holA	2657052..2658093 (-)	1042	Protein_2487	DNA polymerase III subunit delta	-
LCH16_RS12870 (LCH16_12870)	-	2658133..2658284 (-)	152	Protein_2488	hypothetical protein	-
LCH16_RS12875 (LCH16_12875)	-	2658325..2658459 (+)	135	WP_003152870.1	YqzM family protein	-
LCH16_RS12880 (LCH16_12880)	comEC	2658478..2660829 (-)	2352	WP_224462759.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LCH16_RS12885 (LCH16_12885)	-	2660830..2661399 (-)	570	WP_003152868.1	ComE operon protein 2	-
LCH16_RS12890 (LCH16_12890)	comEA	2661466..2662080 (-)	615	WP_061860722.1	helix-hairpin-helix domain-containing protein	Machinery gene
LCH16_RS12895 (LCH16_12895)	comER	2662139..2662960 (+)	822	WP_061860723.1	late competence protein ComER	-
LCH16_RS12900 (LCH16_12900)	-	2663029..2663766 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
LCH16_RS12905 (LCH16_12905)	rsfS	2663763..2664119 (-)	357	WP_007408260.1	ribosome silencing factor	-
LCH16_RS12910 (LCH16_12910)	yqeK	2664137..2664697 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LCH16_RS12915 (LCH16_12915)	-	2664687..2665256 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LCH16_RS12920 (LCH16_12920)	yhbY	2665267..2665557 (-)	291	WP_061860725.1	ribosome assembly RNA-binding protein YhbY	-
LCH16_RS12925 (LCH16_12925)	aroE	2665551..2666393 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22263.50 Da
• Isoelectric Point: 6.4888

>NTDB_id=608689 LCH16_RS12890 WP_061860722.1 2661466..2662080(-) (comEA) [Bacillus velezensis strain CSUSB3]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNINLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=608689 LCH16_RS12890 WP_061860722.1 2661466..2662080(-) (comEA) [Bacillus velezensis strain CSUSB3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATAT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.143	96.078	0.549
comEA	Lactococcus lactis subsp. cremoris KW2	35.586	100	0.387
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363