Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LCC91_RS09520 Genome accession   NZ_CP083911
Coordinates   2019033..2020760 (+) Length   575 a.a.
NCBI ID   WP_043703851.1    Uniprot ID   A0A554X341
Organism   Tepidimonas taiwanensis strain LMG 22826     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2014033..2025760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCC91_RS09500 (LCC91_09500) - 2015000..2016160 (-) 1161 WP_082007754.1 DUF3426 domain-containing protein -
  LCC91_RS09505 (LCC91_09505) prmA 2016183..2017103 (-) 921 WP_052231818.1 50S ribosomal protein L11 methyltransferase -
  LCC91_RS09510 (LCC91_09510) accC 2017090..2018442 (-) 1353 WP_043703856.1 acetyl-CoA carboxylase biotin carboxylase subunit -
  LCC91_RS09515 (LCC91_09515) accB 2018453..2018923 (-) 471 WP_043703854.1 acetyl-CoA carboxylase biotin carboxyl carrier protein -
  LCC91_RS09520 (LCC91_09520) pilB 2019033..2020760 (+) 1728 WP_043703851.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LCC91_RS09525 (LCC91_09525) pilC 2020805..2022022 (+) 1218 WP_043703848.1 type II secretion system F family protein Machinery gene
  LCC91_RS09530 (LCC91_09530) - 2022154..2023806 (+) 1653 WP_043703845.1 tetratricopeptide repeat protein -
  LCC91_RS09535 (LCC91_09535) - 2023813..2025450 (+) 1638 WP_058616336.1 response regulator -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63122.63 Da        Isoelectric Point: 5.7712

>NTDB_id=608468 LCC91_RS09520 WP_043703851.1 2019033..2020760(+) (pilB) [Tepidimonas taiwanensis strain LMG 22826]
MATDEVMATMALPGLARALIASGTLTQQQAEGLYGQAQRKRTSFITELIDSAVISPRDLAYTMSAAFATPLIDLDAIDVD
RLPKGLLDPKTCQRFRILPLKRRNNRVSVATADPSDLQAAEKVKFETQLPVDWVIAEVDKLDRLLKSYAASADESLDQIV
GNTFDFDSLAIESPAEEQSDKAAAEVEDTPVVRFLQKMLLDAYSMRASDLHFEPYEHSFRVRFRIDGVLREIASPPVAIK
DRLVSRIKVISKLDISEKRVPQDGRMKLKVGTDKVIDFRVSTLPTLFGEKVVIRILDPSSAQLGIDALGYEEDEKARLLE
AIHRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEINLPGINQVNVNEKAGLTFAAALRAFLRQDPDVIM
VGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPSTLTRLVNMGVAPFNIASSVHLITAQRLARRLCPQCKVPHHDIPRQV
LLDAGFKPEDLEGDWQPYRAVGCPACNEGYKGRVGIYQVMPVSEAMQALILRGASALEIAEQARREGVRTLRESGLRKVK
MGITSLEEVLAVTNL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=608468 LCC91_RS09520 WP_043703851.1 2019033..2020760(+) (pilB) [Tepidimonas taiwanensis strain LMG 22826]
ATGGCGACCGATGAGGTAATGGCGACGATGGCGTTGCCCGGGCTGGCGCGCGCGCTGATCGCGTCCGGCACCTTGACCCA
ACAGCAGGCCGAAGGACTGTATGGACAAGCGCAGCGCAAACGCACCAGCTTCATCACGGAGCTGATCGACAGCGCCGTGA
TTTCCCCGCGGGACTTGGCCTACACGATGTCGGCCGCGTTCGCAACCCCGCTGATCGACCTGGACGCGATCGACGTGGAT
CGGCTGCCCAAGGGGCTGCTGGACCCCAAAACATGCCAGCGCTTTCGCATCCTGCCGCTCAAACGCCGCAACAACCGGGT
CTCGGTGGCCACGGCCGACCCATCGGACCTGCAGGCGGCCGAAAAAGTGAAATTCGAGACTCAGTTGCCGGTCGACTGGG
TCATTGCCGAAGTCGACAAGCTCGACCGACTGCTCAAATCCTACGCCGCCAGCGCCGACGAGTCGCTGGACCAGATCGTC
GGCAACACCTTCGATTTCGACTCCCTGGCGATCGAGTCACCAGCCGAGGAACAATCGGACAAGGCAGCCGCCGAGGTCGA
GGATACGCCGGTCGTGCGGTTTTTGCAAAAAATGCTGCTCGACGCTTACTCGATGCGGGCGTCGGACCTGCATTTCGAGC
CGTACGAGCACAGTTTCCGCGTGCGTTTTCGCATCGATGGGGTGTTGCGCGAAATCGCGTCGCCGCCGGTGGCGATCAAA
GATCGGCTCGTCTCGCGCATCAAGGTCATCTCCAAGCTCGACATCTCGGAAAAGCGTGTGCCGCAAGACGGCCGCATGAA
ACTCAAGGTCGGGACCGACAAGGTCATCGACTTTCGGGTCAGCACGCTGCCCACACTGTTTGGCGAGAAAGTCGTCATCC
GCATTCTCGACCCGAGCAGTGCACAACTGGGCATCGACGCGCTGGGCTACGAGGAAGACGAAAAAGCCCGCTTGCTGGAA
GCCATCCACCGTCCGTATGGGATGGTGCTGGTCACTGGTCCCACCGGCTCGGGCAAAACGGTGTCGCTCTACACCTGCCT
CAACATCCTCAATCAGCCGGGCATCAACATCTCCACTGCGGAGGATCCGGCCGAAATCAACCTCCCCGGCATCAATCAAG
TCAACGTCAACGAAAAAGCGGGGCTGACCTTTGCCGCCGCCCTGCGGGCCTTTTTGCGGCAGGACCCGGACGTGATCATG
GTGGGGGAAATCCGCGACCTGGAAACTGCCGACATCGCCATCAAGGCGGCGCAGACCGGACACCTGGTGCTGTCGACGCT
GCACACCAACGACGCGCCCTCGACGCTGACGCGCCTGGTCAACATGGGCGTGGCGCCGTTCAACATCGCCTCGAGCGTAC
ACCTGATCACCGCCCAGCGCTTGGCGCGTCGGCTGTGCCCGCAATGCAAGGTGCCCCACCACGACATACCGCGGCAAGTG
CTGCTCGATGCCGGCTTCAAACCCGAGGATCTGGAGGGCGACTGGCAGCCGTACCGCGCAGTGGGGTGTCCCGCATGCAA
TGAAGGCTACAAAGGGCGAGTCGGCATTTACCAGGTCATGCCGGTCTCCGAAGCGATGCAGGCGCTCATCTTGCGCGGGG
CCAGCGCACTCGAAATCGCCGAACAAGCGCGCCGCGAAGGGGTGCGCACCCTGCGCGAAAGCGGCCTGCGCAAGGTTAAG
ATGGGAATCACCTCACTGGAAGAGGTCTTGGCTGTCACCAACCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554X341

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.05

97.739

0.548
  pilB Acinetobacter baylyi ADP1

53.697

98.783

0.53
  pilF Neisseria gonorrhoeae MS11

53.996

97.913

0.529
  pilB Legionella pneumophila strain ERS1305867

50

98.087

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

97.913

0.483
  pilB Vibrio campbellii strain DS40M4

47.703

98.435

0.47
  pilB Vibrio cholerae strain A1552

48.27

95.478

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.948

92.87

0.39
  pilF Thermus thermophilus HB27

39.817

94.783

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.727

90.957

0.37