Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA341_RS11070 Genome accession   NZ_CP083626
Coordinates   2441289..2442398 (-) Length   369 a.a.
NCBI ID   WP_042026652.1    Uniprot ID   A0AAU6TAV3
Organism   Aeromonas enteropelogenes strain FDAARGOS_1509     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2436289..2447398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA341_RS11035 (LA341_11035) - 2436731..2437258 (-) 528 WP_042026648.1 hypothetical protein -
  LA341_RS11040 (LA341_11040) - 2437418..2437888 (+) 471 WP_026456196.1 MarR family winged helix-turn-helix transcriptional regulator -
  LA341_RS11045 (LA341_11045) glnK 2438028..2438366 (-) 339 WP_026456195.1 P-II family nitrogen regulator -
  LA341_RS11050 (LA341_11050) arfB 2438568..2438984 (+) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LA341_RS11055 (LA341_11055) - 2439015..2439506 (+) 492 WP_042026649.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LA341_RS11060 (LA341_11060) - 2439857..2440282 (-) 426 WP_042026650.1 hypothetical protein -
  LA341_RS11065 (LA341_11065) yaaA 2440471..2441244 (-) 774 WP_042026651.1 peroxide stress protein YaaA -
  LA341_RS11070 (LA341_11070) pilU 2441289..2442398 (-) 1110 WP_042026652.1 type IVa pilus ATPase TapU Machinery gene
  LA341_RS11075 (LA341_11075) pilT 2442427..2443461 (-) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  LA341_RS11080 (LA341_11080) - 2443501..2444202 (+) 702 WP_042026653.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA341_RS11085 (LA341_11085) proC 2444259..2445083 (+) 825 WP_042026654.1 pyrroline-5-carboxylate reductase -
  LA341_RS11090 (LA341_11090) - 2445095..2445646 (+) 552 WP_026457844.1 YggT family protein -
  LA341_RS11095 (LA341_11095) yggU 2445646..2445939 (+) 294 WP_042026655.1 DUF167 family protein YggU -
  LA341_RS11100 (LA341_11100) - 2445976..2446395 (+) 420 WP_026457681.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41169.16 Da        Isoelectric Point: 6.2710

>NTDB_id=606771 LA341_RS11070 WP_042026652.1 2441289..2442398(-) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1509]
MNLDQMLAELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALTLVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=606771 LA341_RS11070 WP_042026652.1 2441289..2442398(-) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1509]
ATGAATCTGGATCAGATGCTGGCCGAACTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCTCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCGCTGGGCAACGAGCCCCTCGACAAGAAGGAGGCGCTGACCCTGGTCAGGGAGA
CCCTCAGCTCGGACCATTTTGAACGCTACATCCGCACCAAGGAGGCCAACTACGCCATCCATCGTGAGGAGCTGGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAATTGCCTGGCATGGTGCTGCGCCGCATCGAGACTCGCATTCCCACCTTCGA
TGATCTGAGCCTGCCTCCCATATTGCAGGACGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATTGGCTATCGCAACCAGCATGCGGATGGGCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAGCACGGCCGCAGCCTGGTGACCCAGCGCGAGGTGGGGATAGACACCGAATCGTTCGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCGCCAGATGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGCCACCTCTGTCTGGCAACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTTCCCCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGCATCGACGGGCGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCATGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTTTCGGGTCATGAACAGCTGGGATCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377