Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NMD52_RS02060 Genome accession   NZ_CP100914
Coordinates   433477..433944 (-) Length   155 a.a.
NCBI ID   WP_000340167.1    Uniprot ID   A7ZSL1
Organism   Escherichia coli strain ET761     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428477..438944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD52_RS02035 (NMD52_02025) rpsG 428618..429088 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  NMD52_RS02040 (NMD52_02030) fusA 429185..431299 (+) 2115 WP_000124700.1 elongation factor G -
  NMD52_RS02045 (NMD52_02035) tuf 431370..432554 (+) 1185 WP_000031783.1 elongation factor Tu -
  NMD52_RS02050 (NMD52_02040) bfd 432737..432931 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  NMD52_RS02055 (NMD52_02045) bfr 433004..433480 (+) 477 WP_000675515.1 bacterioferritin -
  NMD52_RS02060 (NMD52_02050) pilD 433477..433944 (-) 468 WP_000340167.1 A24 family peptidase Machinery gene
  NMD52_RS02065 (NMD52_02055) rpsJ 434323..434634 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  NMD52_RS02070 (NMD52_02060) rplC 434667..435296 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  NMD52_RS02075 (NMD52_02065) rplD 435307..435912 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  NMD52_RS02080 (NMD52_02070) rplW 435909..436211 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  NMD52_RS02085 (NMD52_02075) rplB 436229..437050 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  NMD52_RS02090 (NMD52_02080) rpsS 437067..437345 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  NMD52_RS02095 (NMD52_02085) rplV 437360..437692 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  NMD52_RS02100 (NMD52_02090) rpsC 437710..438411 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  NMD52_RS02105 (NMD52_02095) rplP 438424..438834 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16984.17 Da        Isoelectric Point: 8.0293

>NTDB_id=606771 NMD52_RS02060 WP_000340167.1 433477..433944(-) (pilD) [Escherichia coli strain ET761]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=606771 NMD52_RS02060 WP_000340167.1 433477..433944(-) (pilD) [Escherichia coli strain ET761]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACTGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361