Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA341_RS11075 Genome accession   NZ_CP083626
Coordinates   2442427..2443461 (-) Length   344 a.a.
NCBI ID   WP_026457847.1    Uniprot ID   A0AAU6TAH8
Organism   Aeromonas enteropelogenes strain FDAARGOS_1509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437427..2448461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA341_RS11045 (LA341_11045) glnK 2438028..2438366 (-) 339 WP_026456195.1 P-II family nitrogen regulator -
  LA341_RS11050 (LA341_11050) arfB 2438568..2438984 (+) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LA341_RS11055 (LA341_11055) - 2439015..2439506 (+) 492 WP_042026649.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LA341_RS11060 (LA341_11060) - 2439857..2440282 (-) 426 WP_042026650.1 hypothetical protein -
  LA341_RS11065 (LA341_11065) yaaA 2440471..2441244 (-) 774 WP_042026651.1 peroxide stress protein YaaA -
  LA341_RS11070 (LA341_11070) pilU 2441289..2442398 (-) 1110 WP_042026652.1 type IVa pilus ATPase TapU Machinery gene
  LA341_RS11075 (LA341_11075) pilT 2442427..2443461 (-) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  LA341_RS11080 (LA341_11080) - 2443501..2444202 (+) 702 WP_042026653.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA341_RS11085 (LA341_11085) proC 2444259..2445083 (+) 825 WP_042026654.1 pyrroline-5-carboxylate reductase -
  LA341_RS11090 (LA341_11090) - 2445095..2445646 (+) 552 WP_026457844.1 YggT family protein -
  LA341_RS11095 (LA341_11095) yggU 2445646..2445939 (+) 294 WP_042026655.1 DUF167 family protein YggU -
  LA341_RS11100 (LA341_11100) - 2445976..2446395 (+) 420 WP_026457681.1 DUF4426 domain-containing protein -
  LA341_RS11105 (LA341_11105) - 2446501..2447697 (-) 1197 WP_042026657.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.98 Da        Isoelectric Point: 6.7440

>NTDB_id=606772 LA341_RS11075 WP_026457847.1 2442427..2443461(-) (pilT) [Aeromonas enteropelogenes strain FDAARGOS_1509]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSAALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=606772 LA341_RS11075 WP_026457847.1 2442427..2443461(-) (pilT) [Aeromonas enteropelogenes strain FDAARGOS_1509]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTGTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGACGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACAGGGAAGTTCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAAAATTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCGCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGTGGATCCGGCGCCGTGTTTCGTACCATCCCCAGTACGGTACTGAGCCTGGA
AGATCTCGATGCCCCCGAGATATTTCGCAAGATTGCCGAATTCCCACGGGGTCTGGTGCTGGTGACGGGCCCGACCGGCT
CGGGCAAATCCACCACTCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTGGTCAACCAGCGGGAAGTGCACCGCGATACCAAGAGTTTCAGCGC
CGCCCTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGGGACCTCGAGACCATCCGACTCG
CCATGACCGCGGCCGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTGTCCGAATCCCTGCGGGCTGTCATCTCCCAGAC
CTTGCTCAAGCGGATCGGCGGCGGCCGGGTTGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGAAACCTCATTC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGTATGCAGACCATGGATCAGAGCCTC
AAACAGCTGGTCAGCCGGGGTGTGGTGGCGTCCCTCGATGCCAAGGCCAAGGCCGTCGACCCCAACAGCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.551

99.709

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.259

99.709

0.77
  pilT Acinetobacter baumannii strain A118

77.259

99.709

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.51

99.709

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

73.235

98.837

0.724
  pilT Legionella pneumophila strain ERS1305867

73.235

98.837

0.724
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407