Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEDC_RS01305 Genome accession   NZ_CP083571
Coordinates   281126..282835 (-) Length   569 a.a.
NCBI ID   WP_004281098.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain ED9-5a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 276126..287835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEDC_RS01275 (ABEDC_0264) - 276245..276568 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  ABEDC_RS01280 (ABEDC_0265) rlmB 276703..277452 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEDC_RS01285 (ABEDC_0266) - 277459..278382 (+) 924 WP_004644926.1 DMT family transporter -
  ABEDC_RS01290 (ABEDC_0267) coaE 278404..279003 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  ABEDC_RS01295 (ABEDC_0268) pilD 279005..279865 (-) 861 WP_004281094.1 A24 family peptidase Machinery gene
  ABEDC_RS01300 (ABEDC_0269) pilC 279865..281094 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  ABEDC_RS01305 (ABEDC_0270) pilB 281126..282835 (-) 1710 WP_004281098.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEDC_RS01310 (ABEDC_0271) tpiA 283113..283904 (+) 792 WP_004281100.1 triose-phosphate isomerase -
  ABEDC_RS01315 (ABEDC_0272) secG 283918..284247 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -
  ABEDC_RS01345 (ABEDC_0278) rimP 285206..285730 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  ABEDC_RS01350 (ABEDC_0279) nusA 285765..287249 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63526.64 Da        Isoelectric Point: 4.7884

>NTDB_id=606222 ABEDC_RS01305 WP_004281098.1 281126..282835(-) (pilB) [Acinetobacter lwoffii strain ED9-5a]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFRNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=606222 ABEDC_RS01305 WP_004281098.1 281126..282835(-) (pilB) [Acinetobacter lwoffii strain ED9-5a]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCATTAGCGCATGCCAAACAAGAAAAAATCGATATTGTGGCTGAGTTAATTAACCAGCAGCAACTTTCTC
CAACCGTCATTGCTGAAACGATTTCAGTCGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCTCAAATT
CTTCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATTCTGCCGATTTTTAGAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCGACCAATATCGAAGCGATTGATGCAGTCCGCTTTTCCACCAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGTACCTTTGAATTCGATGAAGACTTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTACTAATTGATGCGATCCGTATGGGGGCTTCAGACTTACATTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTAGATGGGGTATTACGCCAGATTGCAACACCACCACTACAGCTGGCAAATCGCTTGTCTTCGCGT
CTTAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGAATTCTGGATCCCTCCA
GCGCGATGTTGGGAATTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGCATTAATCAAGTCAACGTCAACAATAAAG
TTGGTCTTACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCGTAATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACCTTGCATACCAATAGTGCGCC
TGAGACCTTGACCCGTTTACGAAACATGGGGGTGCCCTCTTTCAATATTGCCACTTCAGTAAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGTTTGTGTTCACAATGCAAGAAACCTGCAGATATTCCGCAACAAAGCTTACTGGAGATGGGCTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CAGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.805
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.06

99.473

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

49.374

98.243

0.485
  pilF Neisseria gonorrhoeae MS11

48.663

98.594

0.48
  pilB Vibrio campbellii strain DS40M4

48.746

98.067

0.478
  pilB Vibrio cholerae strain A1552

50.48

91.564

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

35.237

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.932

100

0.373
  pilF Thermus thermophilus HB27

37.168

99.297

0.369