Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K9V45_RS13740 Genome accession   NZ_CP083442
Coordinates   3003040..3005112 (-) Length   690 a.a.
NCBI ID   WP_226305453.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain L5734     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998040..3010112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9V45_RS13725 (K9V45_13725) - 2999668..3001257 (-) 1590 WP_025808183.1 SPOR domain-containing protein -
  K9V45_RS13730 (K9V45_13730) aroB 3001268..3002368 (-) 1101 WP_016704447.1 3-dehydroquinate synthase -
  K9V45_RS13735 (K9V45_13735) aroK 3002517..3003035 (-) 519 WP_009041824.1 shikimate kinase AroK -
  K9V45_RS13740 (K9V45_13740) pilQ 3003040..3005112 (-) 2073 WP_226305453.1 type IV pilus secretin PilQ Machinery gene
  K9V45_RS13745 (K9V45_13745) - 3005126..3005653 (-) 528 WP_169915446.1 pilus assembly protein PilP -
  K9V45_RS13750 (K9V45_13750) - 3005650..3006267 (-) 618 WP_025808189.1 type 4a pilus biogenesis protein PilO -
  K9V45_RS13755 (K9V45_13755) - 3006264..3006830 (-) 567 WP_226305454.1 PilN domain-containing protein -
  K9V45_RS13760 (K9V45_13760) pilM 3006830..3007882 (-) 1053 WP_226305455.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74542.74 Da        Isoelectric Point: 5.2971

>NTDB_id=605855 K9V45_RS13740 WP_226305453.1 3003040..3005112(-) (pilQ) [Pseudomonas chlororaphis strain L5734]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGLPAVASGPAKAAASVGRSIRSVDFQ
RGELGEGNVLIDLSDSGMSPDIQERDGKLVLAFAKARLPEPLRVRLDVKDFATPVQFVSARAEPGKAIITIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=605855 K9V45_RS13740 WP_226305453.1 3003040..3005112(-) (pilQ) [Pseudomonas chlororaphis strain L5734]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCATTGCCGGGGGATCGCCTGGAGTTGAAGTTGACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACCCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCAC
CGGGGCTGCCTGCTGTCGCGTCCGGGCCGGCCAAGGCCGCGGCTTCCGTGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCTGGGGGAGGGCAATGTGCTGATCGACCTCTCCGATTCGGGAATGAGCCCGGATATACAGGAGCGCGACGG
CAAGCTCGTCCTCGCCTTCGCCAAGGCCCGCCTGCCAGAGCCCTTGCGGGTCAGGCTCGACGTCAAGGATTTCGCCACGC
CGGTGCAGTTCGTCAGTGCCCGGGCGGAGCCCGGCAAGGCCATCATCACCATCGAACCTGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTTACGGTGAGCGTCAGGCCGATGACCTCGGGCGATCTGCAGAAGCGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGATCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCTCGCGAACGCCAGGAGCTGGAGTCACAGAAGCAGATAGCCGACCTGGAGCCGCTGCGGCGCGAACTGCTAC
AGGTCAACTATGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGATGAGCGT
GGTTCGATCACCGTCGATGAACGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGAGTCAGCAATGGCACCAACGGC
TCTTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAATTCGCCCTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCTTCCAGTGGGGCGACCTCGGTGTCCTTCAAGGAGGCCTCGCTGTCTTTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTCATAGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGACTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.732
  comQ Acinetobacter baylyi ADP1

39.973

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.899

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.899

100

0.413
  pilQ Legionella pneumophila strain Lp02

37.982

97.681

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.06

97.101

0.37