Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K9V45_RS13760 Genome accession   NZ_CP083442
Coordinates   3006830..3007882 (-) Length   350 a.a.
NCBI ID   WP_226305455.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain L5734     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3001830..3012882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9V45_RS13735 (K9V45_13735) aroK 3002517..3003035 (-) 519 WP_009041824.1 shikimate kinase AroK -
  K9V45_RS13740 (K9V45_13740) pilQ 3003040..3005112 (-) 2073 WP_226305453.1 type IV pilus secretin PilQ Machinery gene
  K9V45_RS13745 (K9V45_13745) - 3005126..3005653 (-) 528 WP_169915446.1 pilus assembly protein PilP -
  K9V45_RS13750 (K9V45_13750) - 3005650..3006267 (-) 618 WP_025808189.1 type 4a pilus biogenesis protein PilO -
  K9V45_RS13755 (K9V45_13755) - 3006264..3006830 (-) 567 WP_226305454.1 PilN domain-containing protein -
  K9V45_RS13760 (K9V45_13760) pilM 3006830..3007882 (-) 1053 WP_226305455.1 pilus assembly protein PilM Machinery gene
  K9V45_RS13765 (K9V45_13765) - 3008099..3010534 (+) 2436 WP_164486060.1 penicillin-binding protein 1A -
  K9V45_RS13770 (K9V45_13770) - 3010731..3011999 (-) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37187.65 Da        Isoelectric Point: 5.1262

>NTDB_id=605856 K9V45_RS13760 WP_226305455.1 3006830..3007882(-) (pilM) [Pseudomonas chlororaphis strain L5734]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLEEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARTVALIDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYRLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=605856 K9V45_RS13760 WP_226305455.1 3006830..3007882(-) (pilM) [Pseudomonas chlororaphis strain L5734]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGAGGAGGCGGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GTAATCCCGAGCGGGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGACCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACCCTGAGTGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGCACCAGTTGACCGAGCACATCCAGCGTCGCTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTTCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGGGGTAGCG
CCTCGGTTCCAGGGCTTGCACCGTTGATCGAGCAACGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCCCCGTCCCTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.545

100

0.549
  comM Acinetobacter nosocomialis M2

54.261

100

0.546
  comM Acinetobacter baylyi ADP1

51.705

100

0.52
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454