Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K8B83_RS04065 Genome accession   NZ_CP082926
Coordinates   862853..864559 (-) Length   568 a.a.
NCBI ID   WP_223419200.1    Uniprot ID   -
Organism   Shewanella inventionis strain D1489     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857853..869559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B83_RS04035 (K8B83_03995) mutT 858459..858872 (-) 414 WP_223419191.1 8-oxo-dGTP diphosphatase MutT -
  K8B83_RS04040 (K8B83_04000) yacG 858894..859103 (-) 210 WP_188741183.1 DNA gyrase inhibitor YacG -
  K8B83_RS04045 (K8B83_04005) zapD 859176..859910 (-) 735 WP_223419193.1 cell division protein ZapD -
  K8B83_RS04050 (K8B83_04010) coaE 859903..860532 (-) 630 WP_223419195.1 dephospho-CoA kinase -
  K8B83_RS04055 (K8B83_04015) pilD 860529..861455 (-) 927 WP_223419196.1 A24 family peptidase Machinery gene
  K8B83_RS04060 (K8B83_04020) pilC 861478..862746 (-) 1269 WP_223419198.1 type II secretion system F family protein Machinery gene
  K8B83_RS04065 (K8B83_04025) pilB 862853..864559 (-) 1707 WP_223419200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8B83_RS04070 (K8B83_04030) - 864654..865082 (-) 429 WP_317846445.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K8B83_RS04075 (K8B83_04035) - 865138..865296 (+) 159 WP_223419202.1 hypothetical protein -
  K8B83_RS04080 (K8B83_04040) nadC 865996..866874 (-) 879 WP_188739664.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B83_RS04085 (K8B83_04045) ampD 867081..867641 (+) 561 WP_223419204.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B83_RS04090 (K8B83_04050) ampE 867728..868579 (+) 852 WP_223419206.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62759.12 Da        Isoelectric Point: 5.6012

>NTDB_id=603630 K8B83_RS04065 WP_223419200.1 862853..864559(-) (pilB) [Shewanella inventionis strain D1489]
MSNTALNLGLSTLFIRKQLLTEEQITAVISTSRKNKTSLVSSIVSSKLLSAREIAELCYEEYGTPLLDLHEFDILNISDE
LLNKKLISKHKCLPLFKRGNRLYIATSDPTNITALEDFQFSLGLHAEAILVEDDKLNKVLEKILEEDISGLGLEGLDEDA
LAGIEVTDTSRDDEPSTDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRQLYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPACKQPEDIPAAELQRLGFQ
EADIAKGITVFKPVGCELCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAKQAKQQGMRDLRQSGLLKVIHGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=603630 K8B83_RS04065 WP_223419200.1 862853..864559(-) (pilB) [Shewanella inventionis strain D1489]
ATGTCCAATACCGCTCTAAATTTAGGTTTATCAACTTTATTTATTCGTAAACAACTCCTAACAGAGGAGCAGATTACGGC
TGTAATCTCCACATCAAGAAAAAATAAGACTTCGCTGGTTAGCTCAATTGTTTCGAGTAAATTATTATCAGCACGTGAAA
TTGCAGAACTCTGCTATGAAGAATACGGTACACCTTTATTAGATTTACATGAATTTGATATTTTAAACATTTCTGATGAG
CTACTTAATAAAAAATTAATAAGTAAACATAAATGTTTGCCACTATTTAAACGTGGTAATCGCCTATATATAGCAACATC
AGACCCAACTAATATAACTGCACTAGAAGACTTTCAATTCAGCCTTGGCTTACACGCCGAAGCGATATTAGTTGAAGATG
ATAAACTTAATAAAGTTTTAGAAAAAATTTTAGAAGAGGATATAAGTGGCTTAGGTCTAGAAGGTTTAGATGAAGATGCA
TTAGCGGGTATAGAAGTTACAGACACCTCTCGCGATGATGAACCATCAACTGATAGCAGCGACGATGCACCAATTGTTAT
CTATATCAATAAAATTCTCACCGATGCCATCCGTAAAGGCGCCTCGGATTTACACTTTGAGCCTTATGAAAAACGCTACC
GTATCCGCTTTCGCATTGACGGCATTTTACATGAAGTATCAGAACCTCCTGTAAATCTATCTGGGCGTATTTCTGCTCGC
TTAAAAGTGATGTCCAAGCTCGATATTGCCGAGCGTCGCGTGCCGCAAGATGGCCGCATTAAAATGAAGCTGTCGCGCAC
CAAATCCATCGATTTCCGTGTCAGTACTTTGCCCACCATTTGGGGCGAAAAGATTGTAATGCGTATTTTGGATTCATCCT
CTGCCCAGCTTGGTATCGAAAAGCTCGGTTACGAAGATGACCAGCGCCAATTATATGAAGAAATGCTCGCCAAACCACAA
GGGATGATTTTAGTCACAGGCCCCACTGGTTCAGGTAAAACCGTATCTTTGTATACTGGGCTTAACATTCTTAATACTGA
AGAACGTAATATTTCAACCGCCGAAGACCCGGTCGAGATTAACCTCGAAGGGGTAAACCAGGTCCACATTAATTTAAAAG
CCGGCTTAACCTTCGCATCCGCTCTGCGCTCATTTTTACGTCAAGACCCTGACGTAGTCATGGTCGGTGAAATCCGCGAC
CTAGAAACCGCTGAAATCGCCATTAAAGCGGCTCAAACTGGCCACCTAGTGTTATCGACATTGCACACCAACTCAGCAGC
CGAAACCTTAACCCGTTTAATTAACATGGGCGTGCCCGGTTATAACATTGCCAGCTCAGTCAATTTAATTATTGCTCAGC
GTCTTGCAAGGCGACTATGCCCAGCCTGTAAACAACCAGAAGATATACCCGCAGCAGAATTACAACGTTTAGGCTTTCAA
GAAGCGGATATTGCCAAAGGCATTACCGTATTTAAACCCGTAGGTTGTGAGCTATGTTCGGGCGGCTATAAAGGCCGCGT
GGGTATTTATGAGGTCATGAAAATGAGTGATGAAATTGCCCGCACCATTATGGAGGGCGGTAACTCACTGCAAATTGCCA
AGCAAGCTAAACAGCAAGGTATGCGCGATTTACGCCAATCTGGCTTATTAAAAGTGATTCATGGCGTCACCAGTATTGCC
GAAATAAACCGTGTCACCAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

99.296

0.581
  pilB Acinetobacter baylyi ADP1

58.467

98.768

0.577
  pilB Legionella pneumophila strain ERS1305867

57.117

98.944

0.565
  pilB Vibrio cholerae strain A1552

53.369

99.296

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

52.398

99.12

0.519
  pilB Vibrio campbellii strain DS40M4

51.327

99.472

0.511
  pilF Neisseria gonorrhoeae MS11

47.434

99.472

0.472
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.11

96.127

0.386
  pilF Thermus thermophilus HB27

37.388

98.415

0.368