Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K8O89_RS17850 Genome accession   NZ_CP082852
Coordinates   4014662..4016380 (-) Length   572 a.a.
NCBI ID   WP_019232345.1    Uniprot ID   A0AAX0WU43
Organism   Legionella anisa strain FDAARGOS_1480     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4009662..4021380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O89_RS17830 (K8O89_17795) - 4010537..4011721 (-) 1185 WP_019232349.1 aminotransferase class V-fold PLP-dependent enzyme -
  K8O89_RS17835 (K8O89_17800) - 4011751..4012518 (-) 768 WP_019232348.1 HAD family hydrolase -
  K8O89_RS17840 (K8O89_17805) - 4012540..4013400 (-) 861 WP_019232347.1 A24 family peptidase -
  K8O89_RS17845 (K8O89_17810) pilC 4013430..4014650 (-) 1221 WP_019232346.1 type II secretion system F family protein Machinery gene
  K8O89_RS17850 (K8O89_17815) pilB 4014662..4016380 (-) 1719 WP_019232345.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8O89_RS17855 (K8O89_17820) - 4016621..4017412 (+) 792 WP_019232344.1 methyltransferase domain-containing protein -
  K8O89_RS17860 (K8O89_17825) - 4017463..4017822 (-) 360 WP_136344279.1 hypothetical protein -
  K8O89_RS17865 (K8O89_17830) - 4017926..4018507 (-) 582 WP_019232342.1 hypothetical protein -
  K8O89_RS17870 (K8O89_17835) - 4018677..4019012 (+) 336 WP_040522024.1 DUF1820 family protein -
  K8O89_RS17875 (K8O89_17840) - 4019050..4019610 (+) 561 WP_019232340.1 hypoxanthine-guanine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62970.58 Da        Isoelectric Point: 6.2070

>NTDB_id=603068 K8O89_RS17850 WP_019232345.1 4014662..4016380(-) (pilB) [Legionella anisa strain FDAARGOS_1480]
MLGSNEFKLHGMGQLFVLEKLLDKTQAVEFCKNASAEKMSLVQYLVKNNILSATQIALTAAHNFGAPMLDLDSIDIDAIP
VSLVNEKLIRRHSMVPLFYRGNNLYLATEDPSKQVSLKEIQFHTGLNTHAIIVEADKLSTLIDHLLTVQETQGLSEFVGD
ATEFDGIIINEEEEHETSIVTSITEDAPIVKFVNKILLDAIKQGASDIHFEPYEKEYRIRYRQDGILHEIATPPGSLSTR
ITARIKIMSNLDISERRIPQDGGFKMKISKTRSIDFRVSTCPTTSGEKVVMRILDPGSAKLGIEALGFSPIQKENFVHSI
ERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKVPGINQVNINPKAGLTFSNALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNSSAETLNRLVNMGIATFNVASSVTLIIAQRLARKLCEHCKVLRDDFNKPGLL
ELGFSETDLEGIKLYKAGGCAKCTNGYRGRIGLFEVLPMTKKLGQIIMSGGNSLDILRIAQEEGMITIYQSGLEKVKQGI
TTIEEVNRVTVD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=603068 K8O89_RS17850 WP_019232345.1 4014662..4016380(-) (pilB) [Legionella anisa strain FDAARGOS_1480]
ATGTTAGGAAGCAATGAATTTAAATTACATGGAATGGGACAATTGTTTGTTCTTGAGAAGCTTTTGGATAAAACACAAGC
GGTTGAATTCTGCAAAAATGCTTCCGCGGAAAAAATGTCCTTAGTTCAATACCTTGTAAAAAACAACATTTTATCGGCGA
CACAAATTGCACTAACAGCAGCACATAATTTCGGCGCTCCGATGCTTGATTTGGACAGTATTGATATTGATGCCATTCCT
GTGAGTTTGGTCAATGAAAAGCTGATTCGCCGCCACTCAATGGTTCCTTTATTTTATCGTGGAAATAATTTATACCTTGC
AACAGAAGACCCAAGCAAACAAGTTTCATTAAAAGAAATCCAATTCCATACGGGACTCAATACCCATGCAATTATAGTTG
AGGCAGATAAACTTAGCACATTAATTGATCATTTGCTTACAGTACAAGAAACTCAGGGTTTGTCTGAATTTGTTGGCGAT
GCTACTGAATTTGATGGAATTATTATTAACGAGGAAGAAGAGCATGAAACAAGTATTGTTACCTCCATAACAGAGGATGC
GCCTATTGTTAAATTCGTTAATAAAATACTTCTGGATGCGATTAAACAAGGTGCTTCAGACATCCATTTTGAACCCTATG
AAAAAGAATATCGTATACGCTATCGACAAGATGGTATTTTACATGAAATCGCAACGCCACCTGGAAGCTTATCTACGCGC
ATTACCGCACGAATAAAAATCATGTCCAATTTGGATATTTCAGAGAGACGAATTCCACAAGATGGTGGTTTTAAGATGAA
AATATCCAAAACCAGATCCATTGATTTTCGTGTAAGTACCTGCCCTACTACTTCAGGTGAGAAAGTTGTGATGCGGATAT
TAGATCCAGGTTCAGCAAAATTGGGTATTGAAGCTTTAGGTTTCAGTCCAATACAAAAGGAAAATTTTGTACATTCAATC
GAACGCCCTCAAGGAATGATCCTGGTCACTGGACCAACAGGAAGTGGTAAAACCGTAACATTGTACACTGCATTGAATAT
CCTCAATACTAAAGAAGTGAATATTTCCACTGCTGAAGACCCTGTTGAAATTAAAGTGCCCGGAATCAATCAGGTTAATA
TCAATCCTAAAGCAGGACTCACATTTTCTAATGCCTTACGCGCATTTTTACGGCAAGATCCTGACATCATAATGGTTGGG
GAAATACGTGATTTGGAAACAGCTGAAATAGCGGTTAAAGCAGCACAAACAGGGCATTTGGTACTCTCAACGTTACATAC
GAATAGCTCTGCTGAAACATTAAACCGTTTGGTCAATATGGGAATCGCAACATTTAATGTAGCAAGCTCAGTAACACTTA
TTATTGCACAACGCTTAGCCCGAAAATTATGTGAACATTGTAAAGTATTAAGAGATGATTTCAACAAACCGGGCTTGTTG
GAATTGGGCTTTTCAGAAACTGATTTAGAGGGAATTAAATTATATAAAGCTGGAGGATGCGCCAAGTGCACTAATGGATA
CCGTGGGAGAATAGGACTGTTTGAAGTATTACCTATGACTAAAAAACTTGGTCAAATCATCATGTCGGGAGGAAATTCTT
TAGATATATTAAGAATCGCCCAGGAAGAAGGCATGATTACTATTTATCAATCGGGACTTGAGAAAGTAAAACAAGGCATT
ACCACTATAGAGGAAGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

79.371

100

0.794
  pilB Acinetobacter baylyi ADP1

50.611

100

0.507
  pilB Acinetobacter baumannii D1279779

51.146

99.126

0.507
  pilB Vibrio cholerae strain A1552

48.188

96.503

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.892

98.427

0.462
  pilF Neisseria gonorrhoeae MS11

45.664

98.776

0.451
  pilB Vibrio campbellii strain DS40M4

46.486

97.028

0.451
  pilF Thermus thermophilus HB27

41.155

93.881

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

92.483

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

38.208

95.629

0.365