Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6752_RS02965 Genome accession   NZ_CP082315
Coordinates   610349..612034 (-) Length   561 a.a.
NCBI ID   WP_213880946.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain ZLV3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 605349..617034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6752_RS02940 (K6752_02940) yacG 606432..606626 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K6752_RS02945 (K6752_02945) zapD 606785..607525 (-) 741 WP_005373086.1 cell division protein ZapD -
  K6752_RS02950 (K6752_02950) coaE 607553..608167 (-) 615 WP_213880942.1 dephospho-CoA kinase -
  K6752_RS02955 (K6752_02955) pilD 608168..609037 (-) 870 WP_213880944.1 prepilin peptidase Machinery gene
  K6752_RS02960 (K6752_02960) pilC 609102..610325 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  K6752_RS02965 (K6752_02965) pilB 610349..612034 (-) 1686 WP_213880946.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6752_RS02970 (K6752_02970) - 612034..612492 (-) 459 WP_064368760.1 pilin -
  K6752_RS02975 (K6752_02975) nadC 612754..613641 (-) 888 WP_017820396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6752_RS02980 (K6752_02980) ampD 613734..614285 (+) 552 WP_005386614.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6752_RS02985 (K6752_02985) pdhR 614691..615458 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62120.02 Da        Isoelectric Point: 5.8153

>NTDB_id=601329 K6752_RS02965 WP_213880946.1 610349..612034(-) (pilB) [Vibrio alginolyticus strain ZLV3]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSVFGLSCASLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSKQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=601329 K6752_RS02965 WP_213880946.1 610349..612034(-) (pilB) [Vibrio alginolyticus strain ZLV3]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCAGGGATACTGACTTTTTCCCAAGAAGAACTGTTACTTGAGCAAGC
TAAAGCTTCTGGTATATCAATGCCGGAGGCTTTGCTCAATTCTGGCTTTTTTCAATCTCATGAATTAACGGAGCACCTAA
GCTCTGTTTTCGGGCTTTCTTGCGCTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACACTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACCTCATCAACTTTGATTCTCGCGGTTGCCGATCCAACCAATCT
TCAAGCCGAAGACGACTTTCGTTTTGCAACAGGCTTGCAAGTCGAACTTGTTCTGGCTGATTTTCGCGAACTGACCGCTG
CGATTCGGCGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGCTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGTGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCGAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCAAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGGCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGATTGAGTATTCTCAACAAGCCAGAAATCAA
CATTTCCACAGCAGAAGACCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCACTGCGCTCTTTCTTACGTCAAGACCCCGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGTCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCAGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

93.405

100

0.934
  pilB Vibrio parahaemolyticus RIMD 2210633

93.048

100

0.93
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.538

92.692

0.478
  pilB Legionella pneumophila strain ERS1305867

48.987

96.791

0.474
  pilB Acinetobacter baylyi ADP1

48.708

96.613

0.471
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

36.594

100

0.387
  pilF Thermus thermophilus HB27

36.477

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.136

99.643

0.36