Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6748_RS02855 Genome accession   NZ_CP082307
Coordinates   587106..587537 (-) Length   143 a.a.
NCBI ID   WP_042521333.1    Uniprot ID   A0A7Y0MVX2
Organism   Vibrio alginolyticus strain HNV2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582106..592537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6748_RS02835 (K6748_02835) coaE 582614..583228 (-) 615 WP_042521328.1 dephospho-CoA kinase -
  K6748_RS02840 (K6748_02840) pilD 583229..584098 (-) 870 WP_017635919.1 A24 family peptidase Machinery gene
  K6748_RS02845 (K6748_02845) pilC 584163..585386 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  K6748_RS02850 (K6748_02850) pilB 585410..587095 (-) 1686 WP_042521331.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6748_RS02855 (K6748_02855) pilA 587106..587537 (-) 432 WP_042521333.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6748_RS02860 (K6748_02860) nadC 587799..588686 (-) 888 WP_042521335.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6748_RS02865 (K6748_02865) ampD 588779..589330 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6748_RS02870 (K6748_02870) pdhR 589736..590503 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14577.45 Da        Isoelectric Point: 4.6894

>NTDB_id=601187 K6748_RS02855 WP_042521333.1 587106..587537(-) (pilA) [Vibrio alginolyticus strain HNV2]
MKNSKQKKQQGFTLIELMIVVAIIGVLAAVAIPAYQDYVKKSEAASALATLKSLITPAELYYQENGTSSAAGLSDLGSVA
SANDLGTLTSAIGSGSGAVPTLEFAFGSNSSMTSSDTLTFSRSATEGWSCARAGTVPAIDGCQ

Nucleotide


Download         Length: 432 bp        

>NTDB_id=601187 K6748_RS02855 WP_042521333.1 587106..587537(-) (pilA) [Vibrio alginolyticus strain HNV2]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATCGAATTGATGATTGTGGTGGCGATTATTGGTGTTTT
GGCAGCCGTCGCCATACCAGCTTATCAAGACTACGTAAAAAAATCAGAAGCCGCATCAGCTCTTGCTACGTTAAAGTCCC
TAATTACTCCGGCTGAACTCTATTATCAAGAAAATGGAACGAGCTCGGCTGCTGGTCTTTCTGATTTAGGTTCAGTAGCT
TCAGCTAACGATTTAGGCACACTAACATCAGCAATTGGATCTGGTTCAGGTGCGGTACCAACTCTAGAATTTGCTTTTGG
TTCAAATAGCTCAATGACCTCGAGTGATACTTTAACTTTCTCTCGTAGTGCAACCGAAGGTTGGTCTTGTGCTCGTGCTG
GTACAGTCCCAGCAATTGATGGCTGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0MVX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

62.676

99.301

0.622
  pilA Pseudomonas aeruginosa PAK

44.776

93.706

0.42
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.606

95.804

0.399
  pilA Vibrio cholerae strain A1552

41.606

95.804

0.399
  pilA Vibrio cholerae C6706

41.606

95.804

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

95.105

0.392