Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K4O48_RS02355 Genome accession   NZ_CP082271
Coordinates   496156..498252 (+) Length   698 a.a.
NCBI ID   WP_222910586.1    Uniprot ID   -
Organism   Pseudomonas sp. DNDY-54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491156..503252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4O48_RS02335 comM 493324..494388 (+) 1065 WP_222910582.1 pilus assembly protein PilM Machinery gene
  K4O48_RS02340 pilN 494388..494960 (+) 573 WP_222910583.1 type 4a pilus biogenesis protein PilN -
  K4O48_RS02345 pilO 494957..495580 (+) 624 WP_222910584.1 type 4a pilus biogenesis protein PilO -
  K4O48_RS02350 pilP 495577..496104 (+) 528 WP_222910585.1 type 4a pilus biogenesis lipoprotein PilP -
  K4O48_RS02355 pilQ 496156..498252 (+) 2097 WP_222910586.1 type IV pilus secretin PilQ Machinery gene
  K4O48_RS02360 aroK 498260..498805 (+) 546 WP_222910587.1 shikimate kinase AroK -
  K4O48_RS02365 aroB 498829..499932 (+) 1104 WP_222910588.1 3-dehydroquinate synthase -
  K4O48_RS02370 - 499942..501447 (+) 1506 WP_222910589.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 75051.98 Da        Isoelectric Point: 4.7063

>NTDB_id=600683 K4O48_RS02355 WP_222910586.1 496156..498252(+) (pilQ) [Pseudomonas sp. DNDY-54]
MNTSLSRLGLSLLAVLISPALLAANLNSLDVASLPGDRVELKLAFDEPVPTPRGYTLDQPARIALDLPGVSSNLASKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESGASPAAVSAAPLAPVAKTAPVAAMAASKSITNID
FRRGEGGAGDVVITLSDPTISPSIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKAEGASIAIEPTGRYD
YLAYQTDDKLTISVKPLSEADAEKRKAEHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALELVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKASDIARLFQSVTDQGGASE
DRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVKWGGNLNLNDNWSAYGRGAEG
LTLEEGGEGEPDIINRPFNLPFVDMGAIGANSGIGLGFVTNNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKILKGS
EVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGIPAIRKNEVNAKVLVTDGETIVI
GGVFSNTQSKSVDKVPFLGDLPFVGRVFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=600683 K4O48_RS02355 WP_222910586.1 496156..498252(+) (pilQ) [Pseudomonas sp. DNDY-54]
ATGAATACCTCTCTATCACGCCTTGGTTTGTCATTGCTGGCAGTACTTATTTCGCCCGCGCTATTGGCTGCAAATCTGAA
TTCACTCGACGTGGCGTCGCTACCTGGCGACCGCGTTGAGCTAAAGTTGGCTTTCGATGAGCCGGTGCCGACGCCGCGCG
GCTACACACTTGACCAGCCAGCGCGCATCGCGCTGGATTTGCCGGGTGTTTCCAGCAATCTTGCATCCAAGAACCGCGAG
CTGGGTGTCGGCAATGCCCGTAGCGTGACGGTCGTTGAGGCGCAAGGCAGAACTCGCCTCATCGTAAACCTGACGGCGCT
CGTTCCATACTCGACCCGTGTGGACGGAAACAATCTATTCGTCGTGCTAGGCGAGAGCGGTGCTTCACCGGCCGCTGTAT
CCGCCGCTCCGCTTGCGCCGGTGGCCAAGACTGCTCCAGTCGCCGCGATGGCAGCGAGCAAGTCGATCACCAATATCGAT
TTCCGTCGCGGAGAGGGTGGTGCGGGCGACGTGGTTATCACGCTATCGGACCCGACGATCAGTCCCAGCATCGAGGAGCA
GGGCGGCAAGATTCGTGTCTCTTTCGCCAAGACACAACTGCCAGAGTCGCTACGCGTCCGGTTGGACGTGCAGGATTTCG
CCACACCGGTCAAGTTCGTCGACTCAAGCAGTAAGGCTGAAGGGGCGAGTATTGCTATCGAGCCGACGGGACGATACGAC
TATCTCGCCTACCAGACCGATGACAAGCTAACAATCAGCGTTAAGCCGCTTAGCGAAGCGGATGCCGAAAAGCGAAAGGC
TGAACACTTCACTTATTCTGGAGAGAAGCTGTCGCTGAACTTTCAGGATATCGATGTGCGGTCTGTGCTGCAGCTGATTG
CAGACTTTACTGACCTTAACTTGGTGGCGAGCGATACCGTACAGGGCAATATTACATTGCGTCTGCAGAATGTACCTTGG
GACCAGGCGTTAGAACTCGTATTGAAGACCAAGGGTCTCGATAAGCGGCAGGTTGGCAATGTGTTGTTGGTCGCGCCGGC
AGATGAGATCGCCGCCCGGGAGCGTCAGGAACTGGAGTCGCAGCGGCAGATTGCTGAGTTGGCGCCCCTGCGTCGCGAGG
TCGTGCAGGTTAACTATGCGAAAGCTTCCGATATTGCTCGGCTGTTCCAGTCCGTTACGGACCAGGGCGGTGCGAGTGAG
GATCGTGGGTCTATCGCTGTAGATGACCGCACGAATAATATCATCGCGTATCAGACTCAAGAGCGTCTCGACGAGCTGCG
ACGGATCGTTGCTCAGCTGGACATTCCGGTGCGCCAAGTGATGATCGAGGCTCGCATCGTTGAGGCGAATGTTGATTACG
ATAAAAGCCTGGGTGTCAAGTGGGGCGGCAACCTGAACCTGAATGACAATTGGAGCGCCTACGGAAGGGGGGCGGAGGGC
TTAACATTAGAAGAGGGTGGGGAAGGTGAGCCGGATATCATTAATCGTCCGTTTAATCTACCGTTTGTAGATATGGGGGC
GATTGGTGCGAACTCCGGTATTGGTCTGGGCTTTGTGACAAACAACGCTATTCTAGACCTGCAACTTTCAGCGATGGAAA
AGACCGGTAATGGTGAGGTCGTTTCGCAGCCTAAGGTTGTAACGGCTGATAAAGAGACGGCGAAGATTTTGAAAGGGTCC
GAGGTTCCTTATCAGGAAGCGAGTTCCAGCGGTGCAACCAGCACTTCGTTCAAAGAGGCGGCCCTGTCGCTTGAGGTTAC
TCCGCAGATTACGCCAGACAACCGGATTATCATGGAGGTGAAGGTCACCAAAGATGAGCCAGATTTCTCGCAGGCGGCTA
GTACTGGCGGTATCCCCGCCATCCGCAAGAACGAAGTCAATGCGAAGGTATTGGTTACCGATGGTGAGACGATCGTGATC
GGCGGCGTGTTCTCCAATACGCAGAGCAAGTCCGTCGACAAGGTTCCGTTCCTAGGCGATCTGCCGTTCGTGGGTCGGGT
GTTCCGTCGCGACTTGGTGCAGGATCGAAAGTCTGAACTGCTGGTGTTCATCACTCCACGGATCATGAATAATCAGGCGA
TCCAAGCCAGTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(531-689)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.106

100

0.741
  pilQ Acinetobacter baumannii strain A118

39.782

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.782

100

0.418
  comQ Acinetobacter baylyi ADP1

41.763

99.14

0.414
  pilQ Legionella pneumophila strain ERS1305867

37.778

96.705

0.365
  pilQ Legionella pneumophila strain Lp02

37.778

96.705

0.365