Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABWED_RS01445 Genome accession   NZ_CP082143
Coordinates   328142..329851 (-) Length   569 a.a.
NCBI ID   WP_004281098.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain ED23-35     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323142..334851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWED_RS01415 (ABWED_0293) - 323262..323585 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  ABWED_RS01420 (ABWED_0294) rlmB 323720..324469 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABWED_RS01425 (ABWED_0295) - 324476..325398 (+) 923 Protein_272 DMT family transporter -
  ABWED_RS01430 (ABWED_0296) coaE 325420..326019 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  ABWED_RS01435 (ABWED_0297) pilD 326021..326881 (-) 861 WP_004281094.1 A24 family peptidase Machinery gene
  ABWED_RS01440 (ABWED_0298) pilC 326881..328110 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  ABWED_RS01445 (ABWED_0299) pilB 328142..329851 (-) 1710 WP_004281098.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABWED_RS01450 (ABWED_0300) tpiA 330129..330920 (+) 792 WP_004281100.1 triose-phosphate isomerase -
  ABWED_RS01455 (ABWED_0301) secG 330934..331263 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -
  ABWED_RS01485 (ABWED_0307) rimP 332222..332746 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  ABWED_RS01490 (ABWED_0308) nusA 332781..334265 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63526.64 Da        Isoelectric Point: 4.7884

>NTDB_id=599473 ABWED_RS01445 WP_004281098.1 328142..329851(-) (pilB) [Acinetobacter lwoffii strain ED23-35]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFRNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=599473 ABWED_RS01445 WP_004281098.1 328142..329851(-) (pilB) [Acinetobacter lwoffii strain ED23-35]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCATTAGCGCATGCCAAACAAGAAAAAATCGATATTGTGGCTGAGTTAATTAACCAGCAGCAACTTTCTC
CAACCGTCATTGCTGAAACGATTTCAGTCGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCTCAAATT
CTTCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATTCTGCCGATTTTTAGAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCGACCAATATCGAAGCGATTGATGCAGTCCGCTTTTCCACCAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGTACCTTTGAATTCGATGAAGACTTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTACTAATTGATGCGATCCGTATGGGGGCTTCAGACTTACATTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTAGATGGGGTATTACGCCAGATTGCAACACCACCACTACAGCTGGCAAATCGCTTGTCTTCGCGT
CTTAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGAATTCTGGATCCCTCCA
GCGCGATGTTGGGAATTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGCATTAATCAAGTCAACGTCAACAATAAAG
TTGGTCTTACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCGTAATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACCTTGCATACCAATAGTGCGCC
TGAGACCTTGACCCGTTTACGAAACATGGGGGTGCCCTCTTTCAATATTGCCACTTCAGTAAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGTTTGTGTTCACAATGCAAGAAACCTGCAGATATTCCGCAACAAAGCTTACTGGAGATGGGCTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CAGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.805
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.06

99.473

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

49.374

98.243

0.485
  pilF Neisseria gonorrhoeae MS11

48.663

98.594

0.48
  pilB Vibrio campbellii strain DS40M4

48.746

98.067

0.478
  pilB Vibrio cholerae strain A1552

50.48

91.564

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

35.237

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.932

100

0.373
  pilF Thermus thermophilus HB27

37.168

99.297

0.369