Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K5K95_RS10875 Genome accession   NZ_CP081946
Coordinates   2375772..2377847 (+) Length   691 a.a.
NCBI ID   WP_222589434.1    Uniprot ID   -
Organism   Pseudomonas sp. DR48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370772..2382847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K95_RS10855 (K5K95_10855) comM 2372984..2374048 (+) 1065 WP_222589430.1 pilus assembly protein PilM Machinery gene
  K5K95_RS10860 (K5K95_10860) - 2374048..2374614 (+) 567 WP_222589431.1 PilN domain-containing protein -
  K5K95_RS10865 (K5K95_10865) pilO 2374611..2375234 (+) 624 WP_222589432.1 type 4a pilus biogenesis protein PilO -
  K5K95_RS10870 (K5K95_10870) - 2375231..2375758 (+) 528 WP_222589433.1 pilus assembly protein PilP -
  K5K95_RS10875 (K5K95_10875) pilQ 2375772..2377847 (+) 2076 WP_222589434.1 type IV pilus secretin PilQ Machinery gene
  K5K95_RS10880 (K5K95_10880) aroK 2377852..2378370 (+) 519 WP_222589435.1 shikimate kinase AroK -
  K5K95_RS10885 (K5K95_10885) aroB 2378521..2379621 (+) 1101 WP_222589436.1 3-dehydroquinate synthase -
  K5K95_RS10890 (K5K95_10890) - 2379632..2381239 (+) 1608 WP_222589437.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74832.11 Da        Isoelectric Point: 5.7840

>NTDB_id=598647 K5K95_RS10875 WP_222589434.1 2375772..2377847(+) (pilQ) [Pseudomonas sp. DR48]
MNRIFSTVGITLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPEPHGYTTESPARIALDLPGVVNQLTSKNRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYNARVEGNKLIVVVGQGATKAPRPPASAPRVATAKPAPVKAYVPANKAIRG
VDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSGA
FDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISDLAPLRRELLQVNYAKAADIAKLFTSVTSAEAK
ADERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSAQNKGNWNTSGVSG
SSTTIGTPGSTSTNSPFVDLGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=598647 K5K95_RS10875 WP_222589434.1 2375772..2377847(+) (pilQ) [Pseudomonas sp. DR48]
ATGAACAGGATTTTCTCAACCGTCGGTATCACGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTCGCCGCGCTGCCGGGTGATCGCGTCGAGTTGAAGTTGTCTTTCGACGGGCCGCCACCGGAGCCCCACG
GATACACGACCGAGTCACCTGCGCGAATCGCGCTCGATCTACCCGGTGTGGTCAATCAGCTGACGAGCAAGAATCGTGAT
CTGGGCGGCGGCAATGCGCGCAGCGCGACGGTGGTGGAGGCTAAAGATCGTACGCGGCTGATCATCAACCTGACCCAGTT
GACCCCGTATAACGCCCGGGTCGAAGGCAACAAACTGATTGTGGTGGTCGGGCAGGGGGCCACCAAGGCGCCTCGACCGC
CGGCCAGTGCGCCCCGCGTCGCGACGGCAAAGCCTGCCCCGGTGAAAGCCTATGTGCCCGCCAACAAAGCCATCCGTGGC
GTGGACTTCCAGCGTGGCACCCAGGGTGAAGGCAATGTGGTGATCGACTTGTCGGATCCTTCAATCGCCCCGGATATCCA
GGAGCGCGACGGCAAGATTATCCTCGGTTTCGCCAGGACCCAATTGCCTGAGCCGTTGCGCGTGCGTCTGGACGTCAAGG
ATTTCGCCACGCCGGTTCAATTCGTCAACGCCAGTGCCACGGGCGACCGGGCGACGATCAGCATCGAGCCCAGCGGAGCG
TTCGATTATTCGACCTACCAGACCGACAACAAGCTGACGGTCAGTATTCGTCCGATGACCGTCGACGACCTGCAAAAGCG
CAACGCCGAGCGTTTTGCCTATACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTACTGCAGT
TGATCGCCGATTTCACCAACCTCAACCTGGTCGCCAGTGACACCGTGCAGGGCGGCATTACCTTGCGTTTGCAAAACGTC
CCATGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAACGTTCTGCTGGTGGC
GCCGGCCGATGAAATCGCCGCTCGCGAACGCCAGGAGCTGGAATCGCAGAAGCAGATTTCCGACCTCGCGCCGCTGCGTC
GAGAGTTGCTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAACTGTTCACTTCGGTAACCAGTGCCGAAGCGAAA
GCCGACGAGCGGGGCTCCATCACGGTTGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTCGACGA
GCTTCGGCGGATCGTCGCGCAACTGGATATACCGGTGCGCCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAATGTCG
ACTATGACAAAAGCCTCGGTGTGCGCTGGGGCGGTTCGGCTCAGAACAAGGGCAACTGGAACACGTCCGGGGTCAGCGGC
TCCTCGACGACCATTGGTACGCCGGGCAGCACCAGCACCAACTCACCGTTTGTCGATCTGGGTGCTGCCGGCAATACCTC
GGGGATCGGCATCGCCTTCATCACCGACAACGTGTTGCTGGATCTGGAGCTGACGGCGATGGAGAAGACCGGCAACGGAG
AAATTGTCTCGCAGCCGAAGGTCGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACCGAGATTCCCTATCAG
GAAGCCAGTTCCAGTGGCGCGACTTCGGTGTCTTTCAAGGAAGCCTCGCTGTCGCTTGAAGTGACGCCGCAGATCACCCC
GGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTCCAGGATGTACCGCCGA
TCAAGAAAAACGAAGTCAATGCCAAGGTGCTGGTCAACGACGGCGAGACCATCGTGATCGGTGGAGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.761
  pilQ Acinetobacter baumannii strain A118

38.697

100

0.421
  pilQ Acinetobacter baumannii D1279779

38.697

100

0.421
  comQ Acinetobacter baylyi ADP1

41.823

100

0.418
  pilQ Legionella pneumophila strain Lp02

38.257

97.974

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.982

97.54

0.37