Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A237_RS25505 Genome accession   NZ_CP081457
Coordinates   5808014..5810119 (-) Length   701 a.a.
NCBI ID   WP_017700276.1    Uniprot ID   A0AAT9SFB4
Organism   Pseudomonas syringae pv. actinidifoliorum ICMP 18803     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5803014..5815119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A237_RS25490 (A237_025395) - 5804639..5806258 (-) 1620 WP_003378845.1 SPOR domain-containing protein -
  A237_RS25495 (A237_025400) aroB 5806269..5807372 (-) 1104 WP_017700275.1 3-dehydroquinate synthase -
  A237_RS25500 (A237_025405) aroK 5807491..5808009 (-) 519 WP_003378847.1 shikimate kinase AroK -
  A237_RS25505 (A237_025410) pilQ 5808014..5810119 (-) 2106 WP_017700276.1 type IV pilus secretin PilQ Machinery gene
  A237_RS25510 (A237_025415) pilP 5810167..5810694 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  A237_RS25515 (A237_025420) pilO 5810691..5811314 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  A237_RS25520 (A237_025425) - 5811311..5811931 (-) 621 WP_017700277.1 PilN domain-containing protein -
  A237_RS25525 (A237_025430) pilM 5811931..5812995 (-) 1065 WP_003378852.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75386.84 Da        Isoelectric Point: 4.9750

>NTDB_id=596461 A237_RS25505 WP_017700276.1 5808014..5810119(-) (pilQ) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPVPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDDNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=596461 A237_RS25505 WP_017700276.1 5808014..5810119(-) (pilQ) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATCGAGCTCAAGCTTGCCTTTGACGCACCGGTGCCCGCGCCTCGCG
GCTACACCACCGCGCAGCCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCCCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
CGCGCCTTACAGCTCCCGCGTCGACGGCAACAATCTGTTCGTGGTCATTGGCCAGGGTGCCGCCGCAGCGCAGGCTTCCC
AGCCGACCACTGCGATGGGTGCGCAACGGGTAGCAGTGCCGCCACCTGTGCCGGCGTCTGCCAAGCCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGCGGTGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGAGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGACTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGATTACTCTGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTACGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGTTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCTAAGCTGTTTCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTCGACGACCGCACCAACAATATCATTGCTTACCA
GACCCAGGACCGTCTCGACGAACTGCGTCGCATCGTTTCGCAACTGGACATCCCGGTGCGCCAGGTGATGATCGAAGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAACAGTTGGGCGTGCGCTGGGGCGGTTCGACCAACACCAGCGGCAGCGGG
AAATGGACGACTTACGGGCTGGATGACAATGGCGATGAGGCCGGTAACACGAGCGGCAATCTGACTTCCAACGTTCCGTT
CGTGGACCTGGGGGCCGCCGGGGCGACGTCCGGCATCGGCCTGGGCTTTGTGACCAACAACACCTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCAAAG
ATCCTCAAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTGAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.614

100

0.749
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii strain A118

38.336

100

0.401
  pilQ Acinetobacter baumannii D1279779

38.336

100

0.401
  pilQ Legionella pneumophila strain Lp02

38.841

98.431

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.653

97.432

0.377