Detailed information NTG0596462

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A237_RS25525
• Genome accession: NZ_CP081457
• Coordinates: 5811931..5812995 (-)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidifoliorum ICMP 18803
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5806931..5817995

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A237_RS25500 (A237_025405)	aroK	5807491..5808009 (-)	519	WP_003378847.1	shikimate kinase AroK	-
A237_RS25505 (A237_025410)	pilQ	5808014..5810119 (-)	2106	WP_017700276.1	type IV pilus secretin PilQ	Machinery gene
A237_RS25510 (A237_025415)	pilP	5810167..5810694 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
A237_RS25515 (A237_025420)	pilO	5810691..5811314 (-)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
A237_RS25520 (A237_025425)	-	5811311..5811931 (-)	621	WP_017700277.1	PilN domain-containing protein	-
A237_RS25525 (A237_025430)	pilM	5811931..5812995 (-)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
A237_RS25530 (A237_025435)	-	5813205..5815646 (+)	2442	WP_370445385.1	penicillin-binding protein 1A	-
A237_RS25535 (A237_025440)	-	5816087..5817355 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=596462 A237_RS25525 WP_003378852.1 5811931..5812995(-) (pilM) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=596462 A237_RS25525 WP_003378852.1 5811931..5812995(-) (pilM) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACTAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGTTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGTTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGACTACCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCAGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469