Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K3169_RS02890 Genome accession   NZ_CP081201
Coordinates   648681..650783 (+) Length   700 a.a.
NCBI ID   WP_255877728.1    Uniprot ID   -
Organism   Pseudomonas phytophila strain ICMP 23753     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 643681..655783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3169_RS02870 (K3169_02845) pilM 645850..646914 (+) 1065 WP_231675814.1 pilus assembly protein PilM Machinery gene
  K3169_RS02875 (K3169_02850) - 646914..647480 (+) 567 WP_027901984.1 PilN domain-containing protein -
  K3169_RS02880 (K3169_02855) pilO 647477..648100 (+) 624 WP_122394928.1 type 4a pilus biogenesis protein PilO -
  K3169_RS02885 (K3169_02860) pilP 648097..648624 (+) 528 WP_099268005.1 type 4a pilus biogenesis lipoprotein PilP -
  K3169_RS02890 (K3169_02865) pilQ 648681..650783 (+) 2103 WP_255877728.1 type IV pilus secretin PilQ Machinery gene
  K3169_RS02895 (K3169_02870) aroK 650788..651306 (+) 519 WP_027901980.1 shikimate kinase AroK -
  K3169_RS02900 (K3169_02875) aroB 651435..652538 (+) 1104 WP_263269881.1 3-dehydroquinate synthase -
  K3169_RS02905 (K3169_02880) - 652548..654167 (+) 1620 WP_263269882.1 AAA family ATPase -

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75785.09 Da        Isoelectric Point: 5.0502

>NTDB_id=595624 K3169_RS02890 WP_255877728.1 648681..650783(+) (pilQ) [Pseudomonas phytophila strain ICMP 23753]
MTRILSAIGVSLWIAMISPMLQAATLKTLDVAALPGDRIELKLTFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRE
LGSGNARSVVVVQANDRTRVIVNMTTLAPYSSRVDGNNLFIVIGQGATAAQASQPSTAQGAARVAVPPSAPARPKAYAPT
GKAIRNVDFQRGELGEGNVIIDLTDSSVAPDIQEQGGKIRVDFAKTQLPEKLRVRLDVKDFATPVQFVNATATGDKASIT
IEPSGPFDYSAYQTESKLTISVRPLTNEDLDRRNAEKLVYTGEKLSLNFQDIDVRSVLQLIADFTSLNLVASDTVQGGIT
LRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQSV
TSAESKVDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKALGVRWAGDTNRNNNHK
WSGYGLDDNGDEAGNTGDALTSNVPFVDLGAANATSGIGIAFMTNNALLDLELSAMERTGNGEIVSQPKVVTSDKETAKI
LKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNMVQGVPPIRKNEVNAKVLVADGETI
VIGGVFSNTQTKTVDKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=595624 K3169_RS02890 WP_255877728.1 648681..650783(+) (pilQ) [Pseudomonas phytophila strain ICMP 23753]
ATGACCAGGATTCTTTCGGCTATCGGTGTATCGCTATGGATTGCGATGATTTCGCCGATGCTCCAGGCCGCCACTCTCAA
GACGCTGGATGTTGCGGCGCTGCCCGGTGACAGGATCGAGCTCAAGCTGACTTTTGATGCGCCGGTCCCTGCCCCCAGAG
GCTATACCACTGAACAGCCAGCGCGTATCGCACTCGATTTGCCCGGCGTGACCAGTCAGCTGGCGACCAAAAGTCGTGAG
CTGGGCAGTGGCAATGCCCGCAGTGTAGTCGTGGTTCAGGCCAATGATCGCACGCGGGTCATTGTCAACATGACCACACT
TGCGCCTTACAGCTCACGGGTTGATGGCAATAATCTGTTTATCGTGATCGGTCAGGGCGCTACGGCGGCGCAGGCATCGC
AACCCAGTACTGCGCAGGGCGCGGCTCGTGTTGCCGTGCCACCTTCTGCTCCCGCAAGACCCAAGGCTTATGCGCCAACA
GGCAAAGCGATCCGCAATGTCGATTTCCAGCGGGGCGAGTTGGGCGAAGGCAATGTGATCATTGATCTGACGGACTCCAG
TGTTGCTCCTGATATTCAGGAGCAGGGCGGCAAGATTCGCGTCGACTTCGCCAAAACCCAATTGCCTGAAAAGCTGCGGG
TACGACTGGATGTCAAGGACTTCGCGACGCCGGTGCAGTTCGTTAACGCCACTGCGACCGGCGACAAGGCAAGCATCACC
ATCGAGCCATCCGGGCCTTTCGATTACTCCGCCTATCAGACCGAAAGCAAGCTGACCATCAGCGTGCGTCCGTTGACCAA
CGAAGACCTGGACCGCCGCAACGCAGAGAAGCTGGTTTACACCGGTGAAAAACTGTCGCTCAACTTCCAGGACATCGATG
TGCGCTCGGTGCTGCAATTGATCGCAGACTTCACCAGTCTCAACCTGGTCGCCAGTGACACGGTTCAGGGCGGCATTACC
TTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGACAAACGCAAGATCGG
CAACGTTTTGCTGGTCGCACCCGCCGACGAAATTGCGGCCCGCGAGCGTCAGGAGCTGGAGTCTCTCAAGCAGATTGCCG
AGCTGGCACCGCTGCGTCGTGAACTGTTGCAGGTCAACTACGCCAAGGCTGCGGACATCGCCAAACTGTTCCAGTCAGTG
ACCAGCGCCGAGTCGAAAGTCGATGAGCGCGGCTCGATTACCGTGGATGACCGAACCAACAACATCATTGCCTACCAGAC
TCAGGACCGTCTGGACGAGCTGCGCCGCATCGTCTCCCAGCTGGATATTCCCGTGCGTCAGGTGATGATCGAGGCGCGAA
TCGTCGAGGCCAATGTCGGTTACGACAAGGCGCTCGGGGTCCGCTGGGCAGGCGACACCAACCGTAATAACAACCACAAA
TGGAGTGGTTATGGTCTGGATGATAATGGGGACGAAGCCGGTAACACGGGCGATGCGCTGACTTCCAATGTGCCATTCGT
CGATCTGGGCGCAGCCAATGCAACCTCCGGCATTGGTATCGCTTTCATGACCAATAACGCTTTACTCGATCTCGAGCTCA
GCGCAATGGAAAGAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCGAAGATT
CTGAAAGGCACCGAGATTCCTTATCAGGAATCCAGCTCCAGTGGTGCCACGACAGTGTCTTTCAAAGAGGCCTCGCTCTC
ACTGGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACC
TGAACATGGTGCAAGGCGTTCCCCCTATTCGTAAAAACGAAGTCAACGCAAAGGTGCTGGTCGCCGATGGAGAAACCATC
GTAATCGGTGGTGTGTTCTCGAATACGCAAACTAAAACTGTCGATAAGGTGCCATTTCTCGGCGATGTGCCGTATGTTGG
CCGCCTTTTCCGGCGCGATGTGGTCTCGGAAGCGAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACC
AGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(535-691)

Secretin and TonB N terminus short domain (STN)

(308-354)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-447)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.296

100

0.753
  comQ Acinetobacter baylyi ADP1

41.785

100

0.421
  pilQ Acinetobacter baumannii strain A118

39.071

100

0.409
  pilQ Acinetobacter baumannii D1279779

39.071

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.192

98

0.374
  pilQ Legionella pneumophila strain Lp02

38.192

98

0.374