Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K3169_RS02870 Genome accession   NZ_CP081201
Coordinates   645850..646914 (+) Length   354 a.a.
NCBI ID   WP_231675814.1    Uniprot ID   -
Organism   Pseudomonas phytophila strain ICMP 23753     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640850..651914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3169_RS02860 (K3169_02835) - 641760..643028 (+) 1269 WP_027901987.1 malic enzyme-like NAD(P)-binding protein -
  K3169_RS02865 (K3169_02840) - 643182..645641 (-) 2460 WP_099268002.1 penicillin-binding protein 1A -
  K3169_RS02870 (K3169_02845) pilM 645850..646914 (+) 1065 WP_231675814.1 pilus assembly protein PilM Machinery gene
  K3169_RS02875 (K3169_02850) - 646914..647480 (+) 567 WP_027901984.1 PilN domain-containing protein -
  K3169_RS02880 (K3169_02855) pilO 647477..648100 (+) 624 WP_122394928.1 type 4a pilus biogenesis protein PilO -
  K3169_RS02885 (K3169_02860) pilP 648097..648624 (+) 528 WP_099268005.1 type 4a pilus biogenesis lipoprotein PilP -
  K3169_RS02890 (K3169_02865) pilQ 648681..650783 (+) 2103 WP_255877728.1 type IV pilus secretin PilQ Machinery gene
  K3169_RS02895 (K3169_02870) aroK 650788..651306 (+) 519 WP_027901980.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38031.58 Da        Isoelectric Point: 4.6813

>NTDB_id=595623 K3169_RS02870 WP_231675814.1 645850..646914(+) (pilM) [Pseudomonas phytophila strain ICMP 23753]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVIEKNIAELEGVGNALARVLAKARTSVKSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLNARVVDVEAYALERAYGLLSTQLGNGHDKLTVAIVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIRRRYGLSM
EEAGLAKKQGGLPDDYLIEVLQPFKDAVVQQVARSLQFFFAAGQYNAVDYIMLAGGTASVSGLDNLIQQRVGTPTLVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=595623 K3169_RS02870 WP_231675814.1 645850..646914(+) (pilM) [Pseudomonas phytophila strain ICMP 23753]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCTACATCGGTGAAACTCCTTGAATT
GAGTCGTTCCGGCACACGTTACAAAGTCGAGTCCTACGCGGTGGAGCCGCTCCCGGCGAACGCGGTGATCGAGAAGAACA
TTGCCGAGCTCGAAGGCGTCGGAAATGCATTGGCGCGCGTATTGGCCAAGGCGCGTACAAGCGTCAAATCTGTGGCGGTC
GCCGTGGCCGGTTCGGCGGTGATTACCAAGACCATCGAGATGGACGCTGGCCTGTCGGATGACGACATGGAAAACCAGCT
GAAAATCGAGGCTGATCAGTACATTCCCTATCCACTGGAAGAAGTCGCGATCGATTTTGAGGTTCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTTGAAGTCCTGCTGGCCGCCTGCCGTAAAGAAAATGTTGAAGTACGCGAAGCCGCCCTGGCCCTT
GCGGGGCTCAATGCACGGGTCGTCGATGTCGAGGCCTACGCACTGGAGCGTGCCTATGGGTTGTTGTCGACGCAACTGGG
CAACGGTCACGACAAACTGACCGTTGCCATCGTCGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGCACAACGGAC
GCATTATCTATACCCGTGAACAGCTGTTCGGTGGGCGTCAGCTGACCGAGGAAATCCGGCGTCGCTATGGCCTTTCGATG
GAAGAAGCCGGGCTTGCCAAGAAGCAGGGCGGTCTGCCCGATGATTACTTGATCGAAGTGCTGCAGCCATTCAAGGACGC
CGTGGTGCAGCAAGTTGCCCGGTCGCTGCAATTCTTCTTCGCCGCAGGACAATACAACGCCGTGGACTACATCATGCTGG
CCGGTGGTACCGCATCGGTTTCCGGGCTGGACAACCTGATTCAACAGCGCGTCGGCACACCCACTCTGGTGGCCAACCCG
TTTGCAGACATGGCCTTGAGCGCCAAGGTCAATGCTGGCGCCCTGGCCAGCGATGCTCCTGCTTTGATGATCGCGTGCGG
TCTGGCCCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

52.542

100

0.525
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469