Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K3819_RS02000 Genome accession   NZ_CP081200
Coordinates   440182..442260 (+) Length   692 a.a.
NCBI ID   WP_221398528.1    Uniprot ID   -
Organism   Pseudomonas sp. 3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435182..447260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3819_RS01980 (K3819_01980) comM 437396..438460 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  K3819_RS01985 (K3819_01985) - 438460..439026 (+) 567 WP_064592903.1 PilN domain-containing protein -
  K3819_RS01990 (K3819_01990) pilO 439023..439646 (+) 624 WP_085611135.1 type 4a pilus biogenesis protein PilO -
  K3819_RS01995 (K3819_01995) - 439643..440167 (+) 525 WP_039766425.1 pilus assembly protein PilP -
  K3819_RS02000 (K3819_02000) pilQ 440182..442260 (+) 2079 WP_221398528.1 type IV pilus secretin PilQ Machinery gene
  K3819_RS02005 (K3819_02005) aroK 442265..442783 (+) 519 WP_011332081.1 shikimate kinase AroK -
  K3819_RS02010 (K3819_02010) aroB 442936..444036 (+) 1101 WP_011332082.1 3-dehydroquinate synthase -
  K3819_RS02015 (K3819_02015) - 444047..445648 (+) 1602 WP_221398529.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74995.14 Da        Isoelectric Point: 5.5724

>NTDB_id=595591 K3819_RS02000 WP_221398528.1 440182..442260(+) (pilQ) [Pseudomonas sp. 3-2]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAAAPRTAARAPRAAPAAAPVRTFAPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPMTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=595591 K3819_RS02000 WP_221398528.1 440182..442260(+) (pilQ) [Pseudomonas sp. 3-2]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGGGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGCCCGCCCCCGCAGCCCAAGG
GCTACACCACCGACTCGCCGGCACGGATCGCACTCGATCTGCCGGGGGTCGCCAGTCAGTTGGCAAGCAAGACCCGTGAC
CTGGGCAGTGGCAATGCCCGCACGGCCACGGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCGTACGACACCCGGGTCGAAGGCAATACGGTGTTCGTGGTGGTCGGGCAAGGCAGCAAAGCCGCTGCCCCACGTA
CGGCGGCTCGTGCCCCGCGTGCAGCACCGGCGGCAGCGCCGGTCCGCACGTTTGCGCCGACCGCCAGAGCCATTCGCGGC
GTGGATTTCCAGCGTGGCACGGCGGGCGAGGGCAACGTGGTCATCGACTTGTCCGACCCGGCTATCGCCCCGGACATCCA
GGAGCACGACGGCAAGATCATCCTCAATTTCGCCCGTACCCAATTGCCAGAACCGCTGCGGGTACGCCTCGACGTCAAGG
ACTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGGGTCAGCGGTGATCGGGCGACGATCACCGTCGAACCCAGCGGTACT
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGATGACCGTCGATGACCTGCAAAAACG
CAACGCCGATCGCAACAGCTATAACGGCGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTCCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGGATCACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTGGC
GCCGGCGGATGAAATTGCTGCGCGCGAGCGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGCTGCGTC
GTGAGCTATTGCAAGTGAACTACGCCAAAGCCGCCGACATCGCCAAGCTGTTCCAGTCTGTCACCAGCGCCGAGGCGAAG
GTCGACGAGCGGGGTTCTATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
GCTGCGGCGGATTGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCACGGATCGTCGAGGCCAACGTCG
ACTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGTTCGATTCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGTTCGTCGACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGGATCGGCATCGCGTTCATCACCGATAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAC
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCCTCGCTGTCACTGGAAGTGACGCCGCAGATCAC
CCCGGACAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.827

100

0.744
  comQ Acinetobacter baylyi ADP1

41.066

100

0.412
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.481

97.543

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.444

97.254

0.364