Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K3369_RS02990 Genome accession   NZ_CP081178
Coordinates   669675..671756 (+) Length   693 a.a.
NCBI ID   WP_042932443.1    Uniprot ID   -
Organism   Pseudomonas mandelii strain KGI_MA19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664675..676756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3369_RS02970 (K3369_02970) comM 666887..667951 (+) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene
  K3369_RS02975 (K3369_02975) - 667951..668517 (+) 567 WP_010464430.1 PilN domain-containing protein -
  K3369_RS02980 (K3369_02980) pilO 668514..669137 (+) 624 WP_010464427.1 type 4a pilus biogenesis protein PilO -
  K3369_RS02985 (K3369_02985) - 669134..669661 (+) 528 WP_010464425.1 pilus assembly protein PilP -
  K3369_RS02990 (K3369_02990) pilQ 669675..671756 (+) 2082 WP_042932443.1 type IV pilus secretin PilQ Machinery gene
  K3369_RS02995 (K3369_02995) aroK 671761..672279 (+) 519 WP_010464421.1 shikimate kinase AroK -
  K3369_RS03000 (K3369_03000) aroB 672431..673531 (+) 1101 WP_102593630.1 3-dehydroquinate synthase -
  K3369_RS03005 (K3369_03005) - 673542..675140 (+) 1599 WP_221108085.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75099.37 Da        Isoelectric Point: 5.7805

>NTDB_id=595481 K3369_RS02990 WP_042932443.1 669675..671756(+) (pilQ) [Pseudomonas mandelii strain KGI_MA19]
MNRIFSTLGISLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTESPARIALDLPGVVNQLSSKSRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYSARVDGNNLIVVVGQGANNKTPRPTTRAPRAATAMPAPTKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFAKTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDLGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=595481 K3369_RS02990 WP_042932443.1 669675..671756(+) (pilQ) [Pseudomonas mandelii strain KGI_MA19]
ATGAACAGGATTTTCTCAACCCTCGGTATCTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTTGCTGCGCTGCCGGGTGATCGTGTCGAGTTGAAGTTGTCTTTCGATGGCCCGCCCCCACAACCTCACG
GTTATACGACCGAGTCGCCGGCACGAATCGCGCTGGATCTGCCCGGGGTGGTCAATCAGCTTTCGAGCAAGAGTCGTGAG
CTGGGTGGTGGCAATGCCCGTAGCGCGACGGTGGTGGAAGCCAAGGACCGGACGCGGCTAATCATCAATCTGACCCAGTT
GACCCCGTACAGCGCCCGTGTCGACGGCAACAACCTGATTGTGGTGGTCGGGCAAGGCGCCAATAACAAAACGCCTCGGC
CGACTACCCGCGCGCCTCGCGCCGCGACTGCAATGCCCGCCCCGACGAAAGCCTATGCGCCCGTGGGCAAAGCCATTCGC
GGTGTGGATTTCCAGCGCGGTACGCAGGGTGAAGGCAATGTAGTGATTGATCTGTCGGATCCGTCCATCGCCCCGGACAT
CCAGGAGCGCGACGGAAAGATCATCCTGGGATTCGCCAAAACCCAATTGCCAGAACCGTTGCGCGTGCGCCTGGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCACGGGGGATCGGGCCACGATCAGTATCGAGCCTAGTGGA
GCCTTCGATTATTCGACCTACCAGACCGACAACAAGCTGACGGTCAGCATTCGGCCAATGACCGTCGACGACCTGCAAAA
GCGCAACGCCGAACGTTTTGCCTATACCGGTGAAAAACTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGC
AACTGATCGCTGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTTTGCAGAAC
GTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGTAACGTCCTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCGCGTGAACGTCAGGAACTGGAATCGCAGAAGCAGATTTCCGAGCTCGCGCCTTTGC
GTCGCGAATTGCTGCAGGTCAACTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCACGTCGGTGACCAGTGCCGAAGCG
AAAATCGACGAGCGGGGTTCGATCACGGTTGATGAACGGACCAACAACATCATTGCCTACCAGACCCAGGACCGACTCGA
CGAACTTCGTCGCATCGTCGCGCAACTAGATATCCCGGTGCGTCAGGTGATGATCGAGGCTCGAATCGTCGAAGCCAACG
TCGACTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACACATCCGGCGTCAGC
AATGGCTCGTCGACCACTATTGGTACTCCGGGCAGCACCAGCACCAACTCGCCGTTTGTCGACCTGGGCGCTGCGGGCAA
CACCTCGGGGATCGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTGGAGCTGACCGCAATGGAGAAGACCGGTA
ACGGCGAAATCGTCTCACAACCCAAGGTGGTCACGTCCGACAAGGAGACCGCGAAAATTCTCAAGGGCACCGAGATTCCC
TATCAGGAAGCCAGTTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAAGCGTCGCTGTCCCTTGAGGTCACGCCGCAGAT
CACCCCGGACAACCGGATCATCATGGAGGTCAAGGTGACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGATGTAC
CCCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATCGTGATTGGTGGGGTTTTCTCA
AATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.759
  comQ Acinetobacter baylyi ADP1

41.643

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.196

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.196

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.405

97.691

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.982

97.258

0.369