Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   K3369_RS02970 Genome accession   NZ_CP081178
Coordinates   666887..667951 (+) Length   354 a.a.
NCBI ID   WP_010464432.1    Uniprot ID   A0A024EBZ8
Organism   Pseudomonas mandelii strain KGI_MA19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661887..672951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3369_RS02960 (K3369_02960) - 662701..663969 (+) 1269 WP_010464435.1 malic enzyme-like NAD(P)-binding protein -
  K3369_RS02965 (K3369_02965) - 664225..666672 (-) 2448 WP_010464433.1 penicillin-binding protein 1A -
  K3369_RS02970 (K3369_02970) comM 666887..667951 (+) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene
  K3369_RS02975 (K3369_02975) - 667951..668517 (+) 567 WP_010464430.1 PilN domain-containing protein -
  K3369_RS02980 (K3369_02980) pilO 668514..669137 (+) 624 WP_010464427.1 type 4a pilus biogenesis protein PilO -
  K3369_RS02985 (K3369_02985) - 669134..669661 (+) 528 WP_010464425.1 pilus assembly protein PilP -
  K3369_RS02990 (K3369_02990) pilQ 669675..671756 (+) 2082 WP_042932443.1 type IV pilus secretin PilQ Machinery gene
  K3369_RS02995 (K3369_02995) aroK 671761..672279 (+) 519 WP_010464421.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38173.71 Da        Isoelectric Point: 4.6091

>NTDB_id=595480 K3369_RS02970 WP_010464432.1 666887..667951(+) (comM) [Pseudomonas mandelii strain KGI_MA19]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPTNAVVEKNIAELEGVGQALARVLLKAKTNLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLAGQLAVSQEGLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVFRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=595480 K3369_RS02970 WP_010464432.1 666887..667951(+) (comM) [Pseudomonas mandelii strain KGI_MA19]
GTGCTAGGACTCTTCAATAAAAAAGCCAATACGCTTCTGGGCATCGACATCAGCTCCACGTCGGTGAAGTTGCTTGAGCT
AAGTCGCCAGGGTGAGCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCCTTGCCTACCAACGCAGTGGTCGAAAAAAATA
TCGCCGAGCTCGAAGGCGTGGGCCAGGCACTCGCGCGCGTGCTGCTCAAAGCCAAAACCAACCTCAGGAATGTCGCCGTG
GCCGTCGCCGGTTCGGCAGTCATCACCAAAACCATCGAGATGGACGCAGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCATTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTGTCGGTGC
GCAACCCCGAACGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTGGAGGTGCGTGAAGCCGCCCTGGCGCTG
GCCGGCCTGACGGCGCGAGTGGTGGATGTCGAGGCCTATGCGCTTGAGCGGTCGTTCTCCTTGCTGGCGGGGCAACTCGC
GGTCTCTCAGGAGGGGCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGTACAACGGGC
GGATCATCTACACCCGGGAACAATTGTTCGGTGGTCGTCAGCTCACGGAGGAAATCCAGCGTCGTTATGGCCTGACCGTC
GAACAGGCGGGGCTGGCGAAGAAGCAGGGCGGGTTGCCGGATGACTACGTCAGCGAAGTCTTGCAACCGTTTCGCGATGC
GCTGGTGCAGCAGGTTTTCCGGTCGTTGCAGTTCTTCTTCGCATCCGGTCAGTACAACGCGGTCGATCACATCCTGCTGG
CTGGCGGTACCGCGTCGGTCCCGGGGCTTGATCGCCTGATCGAGCAGCGGTTGGGCACGCCAACCCAGGTGGCCAATCCG
TTCTCCGACATGGCATTGAGCAGTAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCAGCACTGATGATCGCTTGCGG
GCTCGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EBZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483