Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K4H28_RS12255 Genome accession   NZ_CP081150
Coordinates   2645646..2647349 (+) Length   567 a.a.
NCBI ID   WP_221005457.1    Uniprot ID   -
Organism   Deefgea tanakiae strain D25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640646..2652349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H28_RS12225 (K4H28_12225) rplS 2640850..2641230 (-) 381 WP_221005452.1 50S ribosomal protein L19 -
  K4H28_RS12230 (K4H28_12230) trmD 2641275..2642036 (-) 762 WP_221005453.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  K4H28_RS12235 (K4H28_12235) rimM 2642169..2642711 (-) 543 WP_308443447.1 ribosome maturation factor RimM -
  K4H28_RS12240 (K4H28_12240) rpsP 2642715..2642957 (-) 243 WP_221005454.1 30S ribosomal protein S16 -
  K4H28_RS12245 (K4H28_12245) ffh 2643168..2644511 (-) 1344 WP_221005455.1 signal recognition particle protein -
  K4H28_RS12250 (K4H28_12250) - 2644594..2645403 (+) 810 WP_221005456.1 inner membrane protein YpjD -
  K4H28_RS12255 (K4H28_12255) pilB 2645646..2647349 (+) 1704 WP_221005457.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K4H28_RS12260 (K4H28_12260) pilC 2647361..2648593 (+) 1233 WP_221005458.1 type II secretion system F family protein Machinery gene
  K4H28_RS12265 (K4H28_12265) pilD 2648586..2649455 (+) 870 WP_221005459.1 A24 family peptidase Machinery gene
  K4H28_RS12270 (K4H28_12270) coaE 2649457..2650056 (+) 600 WP_221005460.1 dephospho-CoA kinase -
  K4H28_RS12275 (K4H28_12275) zapD 2650129..2650887 (+) 759 WP_221005461.1 cell division protein ZapD -
  K4H28_RS12280 (K4H28_12280) - 2650888..2651088 (+) 201 WP_221005462.1 DNA gyrase inhibitor YacG -
  K4H28_RS12285 (K4H28_12285) - 2651159..2651974 (-) 816 WP_221005463.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62078.71 Da        Isoelectric Point: 5.4412

>NTDB_id=595392 K4H28_RS12255 WP_221005457.1 2645646..2647349(+) (pilB) [Deefgea tanakiae strain D25]
MTAPHISGLARLLVQHGRLIEADAEALQTTAASNKISFIEQLIQSKKMSARDVADFSSKTFGYPLLDLDRVDPSYIPQGI
LDAKAMQTQRLVPLYKRGTKLFIGISDITNMAAVENVRFQTNAQVEPIIVEDPKLAVLLGKMIEASGATLKDMAIDEAEL
EMAGGDDQPVQDNAANLEIDDAPVVKYLQKMLMDAINQGASDIHFEPYEKFFRIRYRVDGVLREVATPPLAIKEKISSRI
KVISKLDISEKRIPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKAIILEAIQRPYG
MVLVTGPTGSGKTVSLYTCLNLLNKPDINISTAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRDL
ETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKQPVDIPEAALLSAGFEQS
DLDGSWKAYGPKGCEICKDTGYKGRVGIYQVMPITDDLNRIIMSNGNAIDIADQARREGVLSLREAGLRKVKQGLTSLEE
IMAVTNE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=595392 K4H28_RS12255 WP_221005457.1 2645646..2647349(+) (pilB) [Deefgea tanakiae strain D25]
ATGACCGCACCTCATATTTCTGGGCTTGCACGACTCCTAGTGCAACACGGACGCCTAATCGAAGCCGATGCCGAAGCTTT
ACAAACTACCGCTGCCAGTAACAAAATATCATTTATTGAACAGCTAATTCAAAGCAAGAAAATGTCCGCGCGCGACGTTG
CAGATTTCTCGTCCAAAACATTTGGTTATCCGCTACTGGATCTGGATCGGGTTGATCCAAGCTATATTCCGCAAGGCATT
CTTGACGCCAAAGCGATGCAAACGCAACGCTTAGTTCCGCTCTATAAGCGCGGTACCAAACTTTTTATTGGCATTTCAGA
CATCACCAATATGGCTGCGGTGGAAAACGTCCGCTTCCAAACTAACGCCCAAGTCGAACCCATTATCGTTGAAGACCCAA
AGCTCGCCGTTTTACTTGGTAAAATGATCGAAGCCAGTGGGGCAACCCTCAAAGACATGGCCATCGATGAAGCCGAGCTA
GAAATGGCGGGCGGTGATGACCAACCAGTACAAGACAATGCCGCCAATCTCGAGATTGATGATGCACCCGTCGTTAAATA
CCTACAAAAAATGTTAATGGATGCGATCAATCAAGGTGCATCCGACATCCACTTTGAACCCTACGAAAAATTTTTCCGAA
TCCGCTACCGAGTTGACGGCGTACTACGCGAAGTAGCAACACCACCGCTAGCAATCAAAGAAAAAATCTCTTCGCGCATT
AAAGTCATTTCAAAGTTAGATATTTCAGAAAAACGCATCCCACAAGACGGCCGTATGAAGCTTGTTTTATCTAAAAGTCG
CGCGATTGACTTTCGGGTATCAACACTGCCTACGATGCACGGCGAAAAAATTGTAATGCGGATTTTAGATCCATCTTCCG
CAACACTAGGTATTGATGCCCTAGGCTACGATCCAGATCAAAAAGCAATTATTCTTGAAGCAATTCAGCGCCCCTATGGC
ATGGTATTGGTAACAGGACCCACTGGCTCAGGTAAGACTGTCTCACTTTATACATGCTTAAATTTGCTCAACAAACCTGA
TATCAACATCTCAACCGCAGAAGACCCCTGCGAGATTAATTTGCCTGGCATTAATCAAGTCAATGTAAACGACAAGGCAG
GCCTAACTTTCTCTGCCGCGCTTAAAGCATTTTTGCGGCAAGATCCAGACATCATTATGGTGGGTGAGATTCGCGACCTA
GAAACGGCCGACATTGCCATTAAAGCCGCACAGACCGGCCACATGGTGTTTTCGACCCTACATACCAATGACGCGCCAAC
CACATTGACGCGTATGCTCAATATGGGCATACAGCCCTTTAATATTGCCTCGTCCGTCATTTTAATCACCGCACAGCGCC
TCGCGCGTCGACTATGCAGCTGTAAACAGCCTGTAGATATACCTGAAGCCGCGCTCTTGAGCGCAGGCTTCGAGCAGAGC
GATCTTGATGGCAGCTGGAAAGCTTATGGGCCTAAAGGCTGCGAGATCTGTAAAGACACAGGCTACAAAGGCCGTGTAGG
CATTTACCAAGTCATGCCGATTACGGACGACCTCAATCGCATCATCATGAGTAACGGGAATGCAATTGACATTGCCGATC
AGGCGCGCAGAGAGGGTGTACTATCACTGCGCGAAGCAGGACTAAGAAAAGTAAAACAAGGCCTAACTTCACTTGAAGAA
ATCATGGCCGTGACTAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

100

0.552
  pilB Acinetobacter baylyi ADP1

54.577

100

0.547
  pilF Neisseria gonorrhoeae MS11

54.707

99.295

0.543
  pilB Legionella pneumophila strain ERS1305867

52.297

99.824

0.522
  pilB Vibrio cholerae strain A1552

46.786

98.765

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

46.277

99.471

0.46
  pilB Vibrio campbellii strain DS40M4

44.681

99.471

0.444
  pilF Thermus thermophilus HB27

37.719

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

92.945

0.374