Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   K2O51_RS28170 Genome accession   NZ_CP080767
Coordinates   3339669..3341408 (+) Length   579 a.a.
NCBI ID   WP_041680065.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3334669..3346408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS28140 (K2O51_28140) proB 3334726..3335844 (-) 1119 WP_011299100.1 glutamate 5-kinase -
  K2O51_RS28145 (K2O51_28145) obgE 3335930..3337027 (-) 1098 WP_011299101.1 GTPase ObgE -
  K2O51_RS28150 (K2O51_28150) rpmA 3337150..3337410 (-) 261 WP_011299102.1 50S ribosomal protein L27 -
  K2O51_RS28155 (K2O51_28155) rplU 3337450..3337761 (-) 312 WP_011299103.1 50S ribosomal protein L21 -
  K2O51_RS28160 (K2O51_28160) ispB 3338256..3339185 (+) 930 WP_049796430.1 octaprenyl diphosphate synthase -
  K2O51_RS28170 (K2O51_28170) pilF 3339669..3341408 (+) 1740 WP_041680065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K2O51_RS28175 (K2O51_28175) pilC 3341444..3342718 (+) 1275 WP_011299106.1 type II secretion system F family protein Machinery gene
  K2O51_RS28180 (K2O51_28180) pilD 3342722..3343609 (+) 888 WP_245001889.1 A24 family peptidase Machinery gene
  K2O51_RS28185 (K2O51_28185) coaE 3343678..3344304 (+) 627 WP_140953486.1 dephospho-CoA kinase -
  K2O51_RS28190 (K2O51_28190) zapD 3344519..3345277 (+) 759 WP_011299109.1 cell division protein ZapD -
  K2O51_RS28195 (K2O51_28195) - 3345282..3345473 (+) 192 WP_011299110.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63763.46 Da        Isoelectric Point: 7.7993

>NTDB_id=594842 K2O51_RS28170 WP_041680065.1 3339669..3341408(+) (pilF) [Cupriavidus pinatubonensis strain HN-2]
MTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAADKYQLPLLDLSQYNMNKVPSALAGNREF
HAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQKYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTERKMIEYD
PVAAAAQRPRTGATNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDKIATRIKV
LSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQK
ALLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTCKKPGTIP
REALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVLSLREAGL
LKVKQGVTSLEEVLATTNS

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=594842 K2O51_RS28170 WP_041680065.1 3339669..3341408(+) (pilF) [Cupriavidus pinatubonensis strain HN-2]
ATGACACTTGGTCTCGCCCTTGCACAGAGCCGTCGTATCGCCCCCGCGCTGCTCGCCCAACTTGAGCAGTCGGCACGCGA
AAAGCAGACGCAGCTGATCGACGAGATCGTTGGCACCGGCACCATGACCGCGCACGACGTCGCACTGTTCGCCGCCGACA
AGTATCAGCTTCCACTGCTCGATCTCAGCCAGTACAACATGAACAAGGTGCCGTCCGCGCTGGCCGGCAACCGCGAATTC
CACGCGCACCGCCTGCTGCCGCTGGGGCGGCGCGAGAACCGCCTTGTCGTGGCCATGTCGGACCCGTCCAACCAGGCGGG
ACTCGACGCCATCAAGCAGAAGTACAACCTGCCGCTCGACACCGTGGTGGTGGAGCACGACAAGCTGATGAAGCATGTGC
GTTCCGCCGGCGAAGCGCTGGGCACGCTCAAGGACGTTGCGCCCGGCACGCCCACTGAGCGCAAGATGATCGAATACGAT
CCGGTCGCCGCCGCCGCGCAGCGGCCCCGCACGGGCGCCACCAACGACATCGACGATGCGCCGGTCGTGCGCTTCCTGCA
GAAGCTGCTGACCGAAGCCTTCCACCGCGGCGCGTCGGACTTGCACTTCGAGCCGTTCGAGACCTTCTACCGCATCCGCT
TCCGCGTGGACGGCGTGCTGCACGAGGTCGCGCGGCCGCCGCTCGATATTCGCGACAAGATCGCCACGCGCATCAAGGTG
CTGTCGCGCCTGGACATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGACAA
GGACAAGGACGGCAAGGACAAGGAAACCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCCACGCTCTTCGGCG
AGAAGATCGTGATGCGTATCCTGGAATCATCGTCCGACAAGCTCGATATCGACCAGCTCGGCTACGAGCCCGAACAGAAG
GCGCTGCTGCTGGAAACCATCAAGCGGCCCTACGGCATGATCCTGGTGACCGGGCCGACCGGCAGCGGCAAGACCGTGTC
GCTCTATACGTTCCTGAACCTGCTGAACCAGGGCGATATCAATATCTCGACCGCTGAGGACCCGGCTGAAATCCAGCTGC
CCGGCATCAACCAGGTCAACGTCAACGACAAAGCCGGCCTGACCTTTGCCGCGGCGCTGCGTTCGTTCCTGCGGCAGGAT
CCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGATATCTCGATCAAGGCCGCGCAGACAGGCCACCT
GGTGCTGTCGACGCTGCACACCAACGATGCGCCGACCACGCTCACGCGACTGATGAACATGGGCGTGGCGCCGTTCAACA
TCGCATCGAGCGTGCTGCTGATCACGGCGCAGCGCCTGGCGCGGCGGCTGTGCACCTGCAAGAAGCCCGGCACGATCCCG
CGCGAAGCGCTGCTCGACGCCGGGTTCAAGGAACCGCAGCTCGACGGTAGCTGGCAGCCGTACCATCCGGTCGGCTGCGA
GCGCTGCAATGGCAGCGGCTACAAGGGACGCTGCGGCATCTACCAGGTTATGCCGATCACCGAGGCGATGCAGCAGATCA
TCCTGTCGCACGGTACGGCGCTGCAGATTGCCGAGCAGGCGCGGCGCGACGGCGTGCTATCGTTGCGGGAAGCTGGCCTG
CTCAAGGTCAAGCAAGGGGTCACGTCACTCGAAGAAGTGCTGGCCACCACCAATTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.027

100

0.515
  pilB Acinetobacter baumannii D1279779

50.862

100

0.509
  pilB Acinetobacter baylyi ADP1

50.991

95.855

0.489
  pilB Legionella pneumophila strain ERS1305867

49.374

96.546

0.477
  pilB Vibrio cholerae strain A1552

44.31

100

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

44.345

96.2

0.427
  pilB Vibrio campbellii strain DS40M4

44.689

94.301

0.421
  pilF Thermus thermophilus HB27

38.632

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

97.927

0.38