Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K2O51_RS28180 Genome accession   NZ_CP080767
Coordinates   3342722..3343609 (+) Length   295 a.a.
NCBI ID   WP_245001889.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3337722..3348609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS28160 (K2O51_28160) ispB 3338256..3339185 (+) 930 WP_049796430.1 octaprenyl diphosphate synthase -
  K2O51_RS28170 (K2O51_28170) pilF 3339669..3341408 (+) 1740 WP_041680065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K2O51_RS28175 (K2O51_28175) pilC 3341444..3342718 (+) 1275 WP_011299106.1 type II secretion system F family protein Machinery gene
  K2O51_RS28180 (K2O51_28180) pilD 3342722..3343609 (+) 888 WP_245001889.1 A24 family peptidase Machinery gene
  K2O51_RS28185 (K2O51_28185) coaE 3343678..3344304 (+) 627 WP_140953486.1 dephospho-CoA kinase -
  K2O51_RS28190 (K2O51_28190) zapD 3344519..3345277 (+) 759 WP_011299109.1 cell division protein ZapD -
  K2O51_RS28195 (K2O51_28195) - 3345282..3345473 (+) 192 WP_011299110.1 DNA gyrase inhibitor YacG -
  K2O51_RS28200 (K2O51_28200) - 3345496..3347535 (-) 2040 WP_220631070.1 oligopeptide transporter, OPT family -
  K2O51_RS28205 (K2O51_28205) - 3347615..3348043 (-) 429 WP_140953488.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 31650.71 Da        Isoelectric Point: 7.7439

>NTDB_id=594844 K2O51_RS28180 WP_245001889.1 3342722..3343609(+) (pilD) [Cupriavidus pinatubonensis strain HN-2]
MNPAFPAGGAFALPALAALPPWFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAEVRSDPLPYPDRYNLMVPRSA
CPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLVELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDAD
TQLLPDQITLPLVWLGLLLNLAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP

Nucleotide


Download         Length: 888 bp        

>NTDB_id=594844 K2O51_RS28180 WP_245001889.1 3342722..3343609(+) (pilD) [Cupriavidus pinatubonensis strain HN-2]
ATGAATCCGGCATTCCCCGCCGGCGGCGCCTTTGCGCTGCCGGCGCTGGCGGCACTGCCGCCGTGGTTTCTGGTGGCGGC
AGCCGCCGTCCTCGGCCTGCTGGTCGGCAGCTTCCTGAACGTGGTGATCCACCGGCTGCCGCGCATGATGGAACACGACG
AGGCCAACTACATCGCCGAAGTGCGCAGCGACCCGCTGCCCTACCCCGACCGCTACAACCTGATGGTGCCGCGCTCGGCC
TGCCCCCACTGCGGCCATGCGATCGCGGCCTGGGAGAATATTCCGGTTATCAGCTACCTGTTCCTGCGCGGACGCTGCTC
GTCATGCAAGGCACCGATCGGCATGCGCTATCCGCTAGTGGAGCTGGCGACCGGCGTGCTCAGCGCGCTGGCCGCTTGGC
ACTTCGGCGCGACCTGGCAGGGCGTGGCCGCGCTGGTGCTGCTGTGGGCGCTGATGGCACTGACGATGATCGACGCCGAT
ACGCAGTTGCTGCCCGACCAGATCACGCTGCCGCTGGTGTGGCTGGGCCTGCTGCTGAACCTGGCCGGGCTGTTCGTGCC
GATTGCCGATGCCGTGATCGGCGCCGTGGCCGGCTACCTGCTGCTGTGGAGCGCCTACTGGCTGTTCAAGCTCGTGCGCG
GCAAGGAAGGCATGGGCTACGGCGATTTCAAGCTGATGGCCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCCTTGCCGTCG
CTGGTGCTGCTGTCGTCGGTGGTCGGCGTGGTGTTCGGCGTCGCCAATATCGCGCTGCGCCGCCAGGGACGGGACACGCC
GTTCCCGTTCGGCCCATTCATTGCGGGCGCCGGCGGGCTGGTGCTGTTCTTCGGCCCGGGTGTGCTGCCGCTGCTATTCA
TGCCGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(30-135)

Type IV leader peptidase family (Peptidase_A24)

(145-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

53.986

93.559

0.505
  pilD Vibrio cholerae strain A1552

53.986

93.559

0.505
  pilD Neisseria gonorrhoeae MS11

51.292

91.864

0.471
  pilD Acinetobacter baumannii D1279779

47.407

91.525

0.434
  pilD Acinetobacter nosocomialis M2

47.037

91.525

0.431