Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LDH51_RS20865 Genome accession   NZ_CP097001
Coordinates   4228658..4229098 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain NCCP 15734     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4223658..4234098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDH51_RS20845 aroP 4224656..4226029 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  LDH51_RS20850 ampE 4226072..4226926 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LDH51_RS20855 ampD 4226923..4227474 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LDH51_RS20860 nadC 4227562..4228455 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LDH51_RS20865 pilA 4228658..4229098 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  LDH51_RS20870 pilB 4229108..4230493 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  LDH51_RS20875 hofC 4230483..4231685 (+) 1203 WP_000157227.1 protein transport protein HofC -
  LDH51_RS20880 guaC 4231720..4232763 (-) 1044 WP_001217342.1 GMP reductase -
  LDH51_RS20885 - 4232919..4232963 (-) 45 WP_120795372.1 protein YacM -
  LDH51_RS20890 coaE 4232988..4233608 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=593308 LDH51_RS20865 WP_000360895.1 4228658..4229098(+) (pilA) [Escherichia coli strain NCCP 15734]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=593308 LDH51_RS20865 WP_000360895.1 4228658..4229098(+) (pilA) [Escherichia coli strain NCCP 15734]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363