Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0I31_RS17775 Genome accession   NZ_CP080418
Coordinates   4117217..4118923 (+) Length   568 a.a.
NCBI ID   WP_220079235.1    Uniprot ID   -
Organism   Shewanella spartinae strain FJAT-53681     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4112217..4123923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I31_RS17755 (K0I31_17755) ampD 4112728..4113273 (-) 546 WP_220079231.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0I31_RS17760 (K0I31_17760) nadC 4113559..4114437 (+) 879 WP_220079232.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0I31_RS17765 (K0I31_17765) - 4114483..4116147 (+) 1665 WP_220079233.1 Wzy polymerase domain-containing protein -
  K0I31_RS17770 (K0I31_17770) - 4116622..4116999 (+) 378 WP_220079234.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0I31_RS17775 (K0I31_17775) pilB 4117217..4118923 (+) 1707 WP_220079235.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0I31_RS17780 (K0I31_17780) pilC 4118956..4120227 (+) 1272 WP_220079236.1 type II secretion system F family protein Machinery gene
  K0I31_RS17785 (K0I31_17785) pilD 4120271..4121182 (+) 912 WP_220079237.1 A24 family peptidase Machinery gene
  K0I31_RS17790 (K0I31_17790) coaE 4121203..4121808 (+) 606 WP_011867237.1 dephospho-CoA kinase -
  K0I31_RS17795 (K0I31_17795) zapD 4121817..4122551 (+) 735 WP_011867238.1 cell division protein ZapD -
  K0I31_RS17800 (K0I31_17800) yacG 4122600..4122812 (+) 213 WP_041407493.1 DNA gyrase inhibitor YacG -
  K0I31_RS17805 (K0I31_17805) mutT 4122813..4123202 (+) 390 WP_011867240.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63017.10 Da        Isoelectric Point: 4.8548

>NTDB_id=592769 K0I31_RS17775 WP_220079235.1 4117217..4118923(+) (pilB) [Shewanella spartinae strain FJAT-53681]
MPTTGLHLGLSTLFIRKSLLTEEQMSDAITKSRQTKLSLVSTIVTEKILSAREIAELCYEEYGTPLLDLDEFDISSIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTVALEKVLEEDISALDLDGIDEDS
LSGIEITDTDKHQEEQSDAADDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRELYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEEVPEHELLKLGFT
QAQIDDGFTVFKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIANQAKEQGMRDLRESGLRKVIAGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=592769 K0I31_RS17775 WP_220079235.1 4117217..4118923(+) (pilB) [Shewanella spartinae strain FJAT-53681]
ATGCCTACGACAGGACTGCACCTAGGTCTATCGACTCTGTTCATAAGAAAATCTCTCCTAACCGAAGAACAGATGTCGGA
CGCCATAACTAAGTCTAGGCAAACCAAATTATCTCTTGTATCTACAATTGTTACCGAAAAAATCCTCTCGGCACGAGAAA
TCGCTGAGTTGTGTTATGAAGAATACGGTACGCCGCTACTCGATCTCGATGAATTTGATATATCAAGTATCCCCGAAGAC
TTTCTCAATAAAAAACTAATCGAAAAACACAAATGTCTTCCGCTTTTCAAGCGCGGTAACCGTCTTTACATCGGAACCTC
AGATCCCACTAATATAGCGGCATTAGAAGACTTTCAATTTAGCGCAGGTCTACATGCTGAAGCCATTCTAGTAGAAGACG
ATAAACTTACTGTAGCACTCGAAAAAGTACTCGAAGAAGATATCTCGGCACTCGATTTAGATGGTATAGATGAAGATTCT
CTTTCAGGCATTGAAATCACCGACACCGACAAACACCAAGAAGAGCAAAGTGATGCTGCCGATGATGCTCCAATTGTCAT
CTACATCAACAAAATCCTTACTGATGCCATACGTAAAGGCGCATCAGATTTACATTTCGAACCTTACGAGAAGCGATACC
GCATTCGCTTTCGTATCGATGGTATCTTACACGAAGTATCCGAGCCGCCAGTCAATCTAGCTGGTCGCATCTCAGCTCGC
CTTAAGGTAATGTCTAAGCTTGATATCGCCGAGCGCCGCGTCCCTCAAGATGGTCGAATCAAGATGAAGCTGTCTCGTAC
AAAGTCTATCGACTTTCGTGTCAGCACCTTGCCCACTATCTGGGGCGAAAAAATCGTAATGCGTATATTGGACTCTTCTT
CGGCGCAGCTCGGCATCGAAAAGCTTGGATATGAAGATGATCAAAGAGAACTATACGAAGAAATGCTTGCCAAGCCCCAA
GGAATGATCTTAGTCACAGGCCCAACAGGCTCAGGTAAGACTGTATCTCTCTATACCGGTCTAAATATTCTTAATACTCA
AGAACGTAACATCTCTACTGCAGAAGATCCGGTAGAAATTAACCTTGAAGGCGTGAACCAAGTTCACATTAATCTCAAGG
CAGGTCTTACTTTTGCCTCTGCTCTACGCTCTTTCCTGCGTCAAGACCCTGATGTCGTAATGGTTGGTGAAATACGTGAT
TTGGAAACCGCTGAAATAGCAATCAAAGCAGCGCAAACCGGGCACTTAGTATTATCAACCCTGCATACCAACTCCGCTGC
GGAAACCTTAACCCGTCTAATCAACATGGGGGTGCCCGGATATAACATAGCGAGTTCAGTCAACCTGATTATTGCTCAAC
GTCTCGCGCGAAGACTGTGTACCGAATGTCGTCAACCAGAAGAGGTCCCTGAACACGAACTACTCAAATTAGGCTTTACC
CAGGCCCAAATTGATGACGGATTTACCGTCTTTAAACCTGTTGGCTGCGATCTCTGCTCTGGTGGATATAAGGGCCGAGT
GGGTATTTACGAAGTAATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGAGGCAATTCATTGCAAATTGCCA
ACCAGGCCAAAGAGCAAGGCATGCGCGATCTACGTGAGTCGGGACTTCGCAAAGTCATCGCTGGGGTGACGAGCATTGCT
GAAATCAACCGAGTAACCAGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.127

99.648

0.579
  pilB Acinetobacter baylyi ADP1

58.645

98.768

0.579
  pilB Legionella pneumophila strain ERS1305867

55.477

99.648

0.553
  pilB Vibrio cholerae strain A1552

51.872

98.768

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.265

99.472

0.5
  pilB Vibrio campbellii strain DS40M4

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.726

99.472

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.448

97.535

0.375
  pilF Thermus thermophilus HB27

37.968

98.768

0.375