Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0H79_RS01915 Genome accession   NZ_CP080415
Coordinates   419895..421601 (-) Length   568 a.a.
NCBI ID   WP_220077121.1    Uniprot ID   -
Organism   Shewanella sp. FJAT-52076     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414895..426601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H79_RS01885 (K0H79_01885) mutT 415457..415852 (-) 396 WP_220077115.1 8-oxo-dGTP diphosphatase MutT -
  K0H79_RS01890 (K0H79_01890) yacG 415894..416109 (-) 216 WP_220077116.1 DNA gyrase inhibitor YacG -
  K0H79_RS01895 (K0H79_01895) zapD 416172..416906 (-) 735 WP_220077117.1 cell division protein ZapD -
  K0H79_RS01900 (K0H79_01900) coaE 416899..417516 (-) 618 WP_220077118.1 dephospho-CoA kinase -
  K0H79_RS01905 (K0H79_01905) pilD 417516..418430 (-) 915 WP_220077119.1 A24 family peptidase Machinery gene
  K0H79_RS01910 (K0H79_01910) pilC 418477..419736 (-) 1260 WP_220077120.1 type II secretion system F family protein Machinery gene
  K0H79_RS01915 (K0H79_01915) pilB 419895..421601 (-) 1707 WP_220077121.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0H79_RS01920 (K0H79_01920) - 421656..422156 (-) 501 WP_220077122.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0H79_RS01925 (K0H79_01925) nadC 423749..424606 (-) 858 WP_220077123.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0H79_RS01930 (K0H79_01930) ampD 424931..425503 (+) 573 WP_220077124.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0H79_RS01935 (K0H79_01935) ampE 425587..426447 (+) 861 WP_220066488.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62811.21 Da        Isoelectric Point: 5.4670

>NTDB_id=592682 K0H79_RS01915 WP_220077121.1 419895..421601(-) (pilB) [Shewanella sp. FJAT-52076]
MPTTGLHLGLSTLFIRKKLLTEEQLSEAVTLSRKNRHSLVTTLVASKLLSAREIAELCYEEYGTPLLDLAEFDIKSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLIVALEKILEEDISALDLGGLDEES
LAGIEVADTDKRPENDSEGADDAPIVIYINKILTDAIRRGASDLHFEPYEKRYRIRFRIDGILHEVSEPPINLSGRIAAR
LKVMSKLDIAERRLPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQKQLYLDNLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINNRAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQPEEIPNHELERLGFN
EAQIAEGFTAYKPCGCDLCSGGYKGRVGIYEVMPMSDEIARVIMEGGNSLEIARMAKSQGLRDLRLSGLLKVIQGVTSIA
EVNRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=592682 K0H79_RS01915 WP_220077121.1 419895..421601(-) (pilB) [Shewanella sp. FJAT-52076]
ATGCCAACTACTGGTCTCCACTTAGGATTATCGACGCTTTTTATCCGTAAAAAGCTGCTCACTGAAGAGCAGCTGTCGGA
GGCCGTCACCCTTTCGCGAAAAAACAGGCATTCGCTGGTCACCACCTTGGTGGCCAGCAAACTTTTATCGGCACGGGAAA
TAGCCGAGCTGTGCTATGAGGAATACGGCACCCCGTTGCTGGACTTAGCCGAGTTTGACATCAAGAGCATTCCTGAAGAA
TTCCTCAATAAAAAGCTCATCGAAAAACACCGCTGCCTGCCGCTCTTCAAACGGGGTAACCGCCTCTATATTGGCACCTC
AGACCCCACCAATATTGCGGCTCTCGAAGATTTTCAGTTTTCCGCAGGTCTGCATGCCGAAGCCATTTTGGTGGAAGATG
ACAAGCTGATAGTTGCACTCGAGAAAATTCTAGAGGAAGACATCAGCGCACTGGACTTAGGTGGTCTTGACGAAGAGTCG
CTGGCAGGCATAGAGGTTGCCGATACCGACAAGCGCCCGGAAAACGACAGCGAAGGTGCCGATGATGCACCAATTGTTAT
TTACATTAATAAGATTTTGACTGATGCCATTCGCCGCGGCGCATCGGACTTACACTTCGAGCCCTACGAAAAGCGCTATC
GCATCCGTTTTCGCATCGATGGCATTTTGCACGAAGTATCTGAGCCGCCCATTAATCTTTCCGGACGTATCGCTGCCCGT
TTAAAGGTAATGTCGAAACTCGATATTGCCGAGCGCCGCCTGCCCCAAGACGGCCGCATCAAGATGAAGCTCAGCCGTAC
CAAGAGTATCGACTTTCGGGTAAGTACTCTGCCTACGCTCTGGGGCGAAAAAATTGTAATGCGTATTCTGGATTCATCTT
CTGCCCAGCTTGGGATTGAAAAGCTGGGTTATGAGCCAGATCAGAAACAGCTGTATTTGGATAACCTGGCCAAACCCCAG
GGTATGATTTTGGTAACAGGCCCCACTGGTTCGGGGAAAACCGTATCCCTCTACACTGGTTTGAACATTCTCAATACCGC
CGAGCGCAATATCTCGACAGCTGAAGACCCGGTGGAAATTAACCTGGAAGGTGTAAACCAGGTACATATTAATAACAGAG
CAGGGCTGACATTTGCCTCGGCGCTGCGTTCATTTTTGCGCCAGGACCCGGACGTTGTGATGGTGGGGGAAATACGCGAT
TTGGAAACCGCTGAGATAGCCATTAAGGCAGCGCAAACCGGCCACCTCGTGTTATCTACCCTGCACACAAACTCGGCCGC
CGAAACCCTGACCCGACTGATCAACATGGGCGTACCCGGCTACAACATCGCAAGTTCGGTGAATTTGATCATTGCCCAGC
GTCTTGCCCGGCGCCTTTGCCCCGAGTGCAAACAACCGGAAGAGATCCCCAATCACGAGCTGGAGCGTTTAGGTTTTAAT
GAGGCACAGATTGCTGAAGGCTTCACCGCCTACAAACCCTGTGGCTGCGATTTATGTTCTGGCGGTTATAAAGGCCGGGT
GGGTATTTATGAAGTCATGCCCATGAGTGATGAGATTGCAAGGGTTATCATGGAAGGTGGTAACTCTCTTGAAATCGCTC
GCATGGCCAAGTCTCAGGGATTAAGAGACCTGCGCTTGTCGGGGCTCCTAAAGGTAATACAAGGCGTGACCAGTATTGCC
GAGGTAAACCGCGTCACCAGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.754

98.768

0.57
  pilB Acinetobacter baumannii D1279779

57.041

98.768

0.563
  pilB Legionella pneumophila strain ERS1305867

56.537

99.648

0.563
  pilB Vibrio cholerae strain A1552

52.491

98.944

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.681

99.472

0.514
  pilB Vibrio campbellii strain DS40M4

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

48.319

99.472

0.481
  pilF Thermus thermophilus HB27

39.037

98.768

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

93.662

0.375