Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0H63_RS01980 Genome accession   NZ_CP080414
Coordinates   438757..440463 (-) Length   568 a.a.
NCBI ID   WP_220066485.1    Uniprot ID   -
Organism   Shewanella zhangzhouensis strain FJAT-52072     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433757..445463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H63_RS01950 (K0H63_01950) mutT 434319..434714 (-) 396 WP_220066479.1 8-oxo-dGTP diphosphatase MutT -
  K0H63_RS01955 (K0H63_01955) yacG 434756..434971 (-) 216 WP_220066480.1 DNA gyrase inhibitor YacG -
  K0H63_RS01960 (K0H63_01960) zapD 435034..435768 (-) 735 WP_220066481.1 cell division protein ZapD -
  K0H63_RS01965 (K0H63_01965) coaE 435761..436378 (-) 618 WP_220066482.1 dephospho-CoA kinase -
  K0H63_RS01970 (K0H63_01970) pilD 436378..437292 (-) 915 WP_220066483.1 A24 family peptidase Machinery gene
  K0H63_RS01975 (K0H63_01975) pilC 437339..438598 (-) 1260 WP_220066484.1 type II secretion system F family protein Machinery gene
  K0H63_RS01980 (K0H63_01980) pilB 438757..440463 (-) 1707 WP_220066485.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0H63_RS20180 (K0H63_01985) - 440475..440912 (-) 438 WP_220066486.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0H63_RS01990 (K0H63_01990) nadC 442553..443410 (-) 858 WP_220066487.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0H63_RS01995 (K0H63_01995) ampD 443761..444315 (+) 555 WP_220067779.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0H63_RS02000 (K0H63_02000) ampE 444399..445259 (+) 861 WP_220066488.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62757.12 Da        Isoelectric Point: 5.7407

>NTDB_id=592660 K0H63_RS01980 WP_220066485.1 438757..440463(-) (pilB) [Shewanella zhangzhouensis strain FJAT-52072]
MPTTGLHLGLSTLFIRKKLLSEEQLSEAVALSRKNRHSLVTTLVASKLLSAREIAELCYEEYGTPLLDLAEFDIKSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLIAALEKILEEDISALDLSGIDKDS
LAGIEVADTDKRTENDNDGADDAPIVIYINKILTDAIRRGASDLHFEPYEKRYRIRFRIDGILHEVSEPPINLSGRIAAR
LKVMSKLDIAERRLPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQKQLYLDNLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINNRAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECKQPEEIPNHELERLGFN
EAQIAEGFTAYKPCGCDLCSGGYKGRVGIYEVMPMSDEIARVIMEGGNSLEIARMAKSQGVRDLRLSGLLKVIQGVTSIA
EVNRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=592660 K0H63_RS01980 WP_220066485.1 438757..440463(-) (pilB) [Shewanella zhangzhouensis strain FJAT-52072]
ATGCCAACTACAGGCCTCCACTTAGGGTTATCGACACTTTTTATCCGTAAGAAGCTGCTAAGTGAAGAGCAGCTGTCGGA
GGCCGTCGCTCTATCGCGAAAAAACCGGCATTCGCTGGTCACCACCCTGGTGGCCAGCAAACTTTTATCGGCAAGGGAAA
TAGCCGAGCTCTGCTACGAGGAATATGGCACGCCGCTGCTGGACTTGGCCGAATTTGACATAAAGAGTATTCCGGAAGAG
TTCCTCAATAAAAAACTCATCGAGAAACATCGCTGCCTGCCGCTCTTCAAACGTGGTAACCGCCTCTATATAGGCACCTC
AGACCCCACCAATATCGCTGCTCTCGAAGATTTCCAGTTTTCCGCAGGCCTGCACGCCGAAGCCATTTTGGTGGAAGATG
ACAAGCTGATTGCCGCCCTCGAGAAAATACTCGAAGAAGACATCAGCGCACTGGACTTAAGCGGTATAGATAAAGACTCA
TTGGCGGGCATTGAGGTCGCCGATACCGATAAGCGCACGGAAAATGATAACGACGGCGCTGACGATGCGCCCATTGTTAT
TTACATTAATAAGATTCTCACCGATGCCATTCGCCGCGGCGCATCGGATTTACACTTCGAGCCCTACGAAAAACGCTACC
GCATTCGGTTTCGTATCGATGGCATTTTGCACGAAGTCTCAGAGCCACCCATTAATCTTTCCGGCCGTATCGCTGCCCGC
TTAAAGGTGATGTCGAAACTCGATATTGCCGAGCGCCGCCTGCCACAGGATGGCCGCATCAAGATGAAGCTCAGCCGCAC
CAAGAGTATCGACTTTCGGGTAAGCACCCTGCCTACGCTCTGGGGCGAAAAAATTGTAATGCGTATCCTTGACTCGTCCT
CAGCCCAGCTGGGGATCGAGAAGCTCGGTTATGAGCCAGACCAGAAGCAGCTGTATTTGGATAACCTGGCCAAACCCCAG
GGTATGATTCTGGTAACAGGCCCCACCGGCTCTGGTAAAACTGTATCCCTGTACACAGGTTTGAACATTCTCAATACCGC
CGAGCGCAATATTTCCACAGCGGAAGACCCGGTGGAAATTAACCTGGAAGGTGTAAACCAGGTGCATATCAACAACCGAG
CAGGGCTGACATTTGCATCGGCACTGCGCTCTTTCCTGCGCCAGGACCCTGACGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCTGAGATAGCTATTAAAGCGGCACAAACTGGCCACCTGGTGTTATCTACCCTGCACACAAACTCGGCCGC
CGAAACCCTGACCCGACTGCTCAACATGGGCGTACCCGGCTACAACATTGCAAGTTCGGTGAATTTAATTATCGCCCAGC
GCCTCGCCCGGCGTCTTTGCCCCGAGTGCAAACAACCGGAAGAGATCCCTAATCACGAGCTGGAGCGTTTGGGCTTTAAT
GAGGCACAAATTGCTGAAGGTTTTACCGCCTACAAGCCCTGCGGCTGCGATTTATGTTCCGGTGGTTATAAAGGCCGGGT
GGGTATTTATGAAGTGATGCCCATGAGTGATGAGATAGCAAGGGTTATCATGGAAGGCGGTAACTCGTTGGAGATCGCTC
GCATGGCCAAGTCTCAGGGAGTAAGAGACCTGCGCTTGTCAGGCCTTCTTAAGGTAATACAAGGCGTAACCAGTATTGCT
GAGGTAAACCGCGTCACCAGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.932

98.768

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

99.648

0.563
  pilB Acinetobacter baumannii D1279779

56.964

98.592

0.562
  pilB Vibrio cholerae strain A1552

53.025

98.944

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

51.504

99.472

0.512
  pilB Vibrio campbellii strain DS40M4

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

48.319

99.472

0.481
  pilF Thermus thermophilus HB27

39.394

98.768

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.744

96.127

0.382