Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PCA10_RS26245 Genome accession   NC_021499
Coordinates   5848388..5850487 (-) Length   699 a.a.
NCBI ID   WP_016495119.1    Uniprot ID   S6AX63
Organism   Metapseudomonas resinovorans NBRC 106553     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5843388..5855487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCA10_RS26230 (PCA10_52580) - 5845059..5846699 (-) 1641 WP_016495116.1 AAA family ATPase -
  PCA10_RS26235 (PCA10_52590) aroB 5846709..5847812 (-) 1104 WP_016495117.1 3-dehydroquinate synthase -
  PCA10_RS26240 (PCA10_52600) aroK 5847864..5848382 (-) 519 WP_081664013.1 shikimate kinase AroK -
  PCA10_RS26245 (PCA10_52610) pilQ 5848388..5850487 (-) 2100 WP_016495119.1 type IV pilus secretin PilQ Machinery gene
  PCA10_RS26250 (PCA10_52620) pilP 5850545..5851078 (-) 534 WP_016495120.1 type 4a pilus biogenesis lipoprotein PilP -
  PCA10_RS26255 (PCA10_52630) pilO 5851075..5851698 (-) 624 WP_016495121.1 type 4a pilus biogenesis protein PilO -
  PCA10_RS26260 (PCA10_52640) pilN 5851695..5852318 (-) 624 WP_016495122.1 type 4a pilus biogenesis protein PilN -
  PCA10_RS26265 (PCA10_52650) pilM 5852318..5853382 (-) 1065 WP_016495123.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75162.29 Da        Isoelectric Point: 4.8446

>NTDB_id=59210 PCA10_RS26245 WP_016495119.1 5848388..5850487(-) (pilQ) [Metapseudomonas resinovorans NBRC 106553]
MKSSLSRLGITLLAALLSPALLAANLQALDVAALPGDKVELRLTFDEPVVAPRGYTIEQPARIALDLPGVSSELGTKNRE
LGVGNARSVTVVEAKDRTRLIINLTTLVPYSTRVEGKNLYVLVGDSASAAAPRPVAAAPAPVAAPLSASQPVKNYGRSDR
AIENIDFQRGEQGEGNVVITLSDPSVSPDIQEQGGKIRLDFAKTQLPEPLRVRLDVKDFATPVQFVSASGSGSNSSISIE
PTGFYDYLAYQTDNKLTVSIKPLSQDDVEKRKIERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQRQIAELAPLRRELIQVNYAKAADIAKLFQSVTS
GDPSAAGTDERGSITVDDRTNSIIAYQTQEKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWRGASIGDNNF
VVGGGGNGRPGAGQSGNVNLADAPFVDLGASGATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLVNDGETIV
IGGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=59210 PCA10_RS26245 WP_016495119.1 5848388..5850487(-) (pilQ) [Metapseudomonas resinovorans NBRC 106553]
ATGAAAAGCTCGCTATCGCGCCTCGGTATCACGCTATTGGCCGCCCTGCTGTCGCCCGCGCTGCTGGCCGCGAACCTGCA
GGCGCTGGATGTCGCGGCCCTGCCAGGCGACAAGGTTGAACTCAGGCTGACCTTCGATGAGCCGGTGGTCGCTCCTCGCG
GCTATACCATCGAGCAGCCCGCGCGTATCGCGCTGGACTTGCCGGGTGTATCCAGCGAACTGGGTACCAAGAACCGTGAG
CTGGGTGTGGGCAATGCCCGCAGCGTGACCGTGGTCGAAGCCAAGGATCGCACCCGCCTGATCATCAACCTGACCACGCT
GGTGCCCTATAGCACTCGCGTGGAAGGGAAGAACCTCTACGTGCTGGTCGGTGACAGTGCATCCGCAGCTGCCCCGCGCC
CCGTAGCCGCTGCGCCGGCTCCTGTCGCCGCACCGCTGTCCGCATCGCAGCCGGTGAAGAACTACGGCCGTTCTGATCGC
GCCATCGAGAACATCGACTTCCAGCGCGGTGAGCAGGGCGAGGGCAATGTGGTCATTACCCTCTCCGATCCCAGCGTCAG
TCCGGATATCCAGGAGCAAGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGTTGCCGGAGCCCCTGCGCGTACGCC
TGGATGTGAAGGATTTCGCCACCCCGGTGCAGTTCGTCAGTGCGTCGGGCTCGGGTAGCAATTCCAGCATCAGCATCGAG
CCCACCGGCTTCTACGACTACCTGGCCTATCAGACCGACAACAAGCTGACCGTCAGCATCAAGCCGCTGAGCCAGGATGA
CGTCGAGAAGCGCAAGATCGAGCGTTTCGCCTACTCCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTACGTT
CGGTGCTGCAGCTGATCGCCGACTTCACTGACCTCAACCTGGTGGCGTCGGACACCGTGCAGGGCAACATCACCCTGCGC
CTGCAGAACGTGCCCTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGT
GCTGCTGGTTGCGCCGGCGGACGAGATCGCTGCGCGCGAACGTCAGGAGCTGGAGTCGCAACGGCAGATCGCCGAGCTGG
CGCCCCTGCGTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGT
GGTGATCCGAGTGCGGCCGGCACTGACGAGCGCGGTTCCATCACGGTGGACGATCGTACCAACAGCATCATCGCCTACCA
GACCCAGGAAAAGCTGGATGAGCTGCGTCGTATCGTCACCCAGCTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAAGCCAACGTCGACTACGACAAGTCCCTCGGCGTGCGCTGGCGCGGCGCCAGCATCGGCGACAACAACTTC
GTCGTCGGCGGTGGCGGCAACGGTCGTCCGGGCGCGGGGCAATCTGGCAACGTCAACCTCGCCGACGCACCCTTCGTCGA
CCTGGGCGCTTCCGGCGCCACCTCGAGCATCGGCATTGGCTTCATCACCGACAACACCATCCTCGACCTCGAACTGTCGG
CGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTC
AAGGGCGCCGAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCAGCACCTCCTTCAAGGAAGCGGCGCTGTCCCT
GGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGCGCCGGACTTCGCCA
ACGCGCTAAACGGTGTACCGCCGATCAATAAAAACGAGGTCAACGCCAAGGTACTGGTGAACGATGGTGAAACCATTGTC
ATAGGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTGCCCTTCCTCGGCCG
CATGTTCCGTCGCGACGTGGTTCAGGACAAGAAGTCTGAGCTCCTGGTATTCCTCACTCCCCGCATCATGAACAACCAGG
CTATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-448)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(306-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AX63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.994

100

0.801
  comQ Acinetobacter baylyi ADP1

43.867

99.142

0.435
  pilQ Acinetobacter baumannii D1279779

41.702

100

0.421
  pilQ Acinetobacter baumannii strain A118

41.702

100

0.421
  pilQ Legionella pneumophila strain Lp02

38.439

97.139

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.292

97.139

0.372

Multiple sequence alignment