Detailed information NTG0059211

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCA10_RS26265
• Genome accession: NC_021499
• Coordinates: 5852318..5853382 (-)
• Length: 354 a.a.
• NCBI ID: WP_016495123.1
• Uniprot ID: S6AJN5
• Organism: Metapseudomonas resinovorans NBRC 106553
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5847318..5858382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCA10_RS26240 (PCA10_52600)	aroK	5847864..5848382 (-)	519	WP_081664013.1	shikimate kinase AroK	-
PCA10_RS26245 (PCA10_52610)	pilQ	5848388..5850487 (-)	2100	WP_016495119.1	type IV pilus secretin PilQ	Machinery gene
PCA10_RS26250 (PCA10_52620)	pilP	5850545..5851078 (-)	534	WP_016495120.1	type 4a pilus biogenesis lipoprotein PilP	-
PCA10_RS26255 (PCA10_52630)	pilO	5851075..5851698 (-)	624	WP_016495121.1	type 4a pilus biogenesis protein PilO	-
PCA10_RS26260 (PCA10_52640)	pilN	5851695..5852318 (-)	624	WP_016495122.1	type 4a pilus biogenesis protein PilN	-
PCA10_RS26265 (PCA10_52650)	pilM	5852318..5853382 (-)	1065	WP_016495123.1	pilus assembly protein PilM	Machinery gene
PCA10_RS26270 (PCA10_52660)	-	5853564..5856005 (+)	2442	WP_016495124.1	penicillin-binding protein 1A	-
PCA10_RS26275 (PCA10_52670)	-	5856064..5857332 (-)	1269	WP_016495125.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37890.06 Da
• Isoelectric Point: 4.4858

>NTDB_id=59211 PCA10_RS26265 WP_016495123.1 5852318..5853382(-) (pilM) [Metapseudomonas resinovorans NBRC 106553]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVITKARTGVKSAAV
AVAGSAVITKTIEMESGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGQSARNSERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLSSQLGESHSDLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIADLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=59211 PCA10_RS26265 WP_016495123.1 5852318..5853382(-) (pilM) [Metapseudomonas resinovorans NBRC 106553]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATAAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCGCTCCCACCAAACGCGGTCGTCGAAAAGAACA
TCGCGGAGCTGGAAGGCGTCGGGCAGGCGCTTTCCCGTGTGATCACCAAGGCACGCACCGGCGTCAAGTCGGCCGCCGTC
GCCGTGGCCGGTTCCGCCGTGATCACCAAGACCATCGAGATGGAATCCGGTCTCTCCGACGACGACCTGGAAAACCAGCT
GAAGATCGAGGCGGATCAATATATCCCCTATCCGCTGGAAGAGGTCGCCATCGATTTCGAGGTGCAGGGGCAATCCGCGC
GCAACTCCGAGCGCGTCGATGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTG
GCAGGGCTCACCGCCAAGGTGGTCGATGTCGAGGCCTATGCGCTGGAACGCGCCTACTCGCTCCTTTCCTCCCAGCTCGG
CGAGTCCCATTCCGACCTGACCGTGGCCGTCGTCGATATCGGCGCCACCATGACGACCCTCAGCGTGCTGCACAACGGTC
GCACCATCTATACCCGGGAGCAGCTCTTCGGTGGCCGCCAGTTGACCGAGGAGATCCAGCGTCGCTATGGCCTCTCGGTC
GAAGAAGCTGGCCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTCTGCAACCGTTCAAGGATGC
CGTTGTCCAGCAGGTGTCCCGTTCGCTGCAGTTCTTCTTCGCCGCGGGCCAGTTCAACGATGTCGATTACATCCTCCTGG
CCGGTGGCACGGCGTCCATTGCGGACCTGGATCGCCTCATCCAGCAGAAGATCGGTACCCCGACCCTGGTCGCCAACCCC
TTCGCCGACATGGCGCTGAGCAGCAAGGTCAATGCGGGGGCTCTGGCTAGCGATGCGCCGGCTCTGATGATTGCATGCGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: S6AJN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	52.825	100	0.528
pilM	Legionella pneumophila strain ERS1305867	46.893	100	0.469