Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KX724_RS16540 Genome accession   NZ_CP079106
Coordinates   3622780..3624507 (-) Length   575 a.a.
NCBI ID   WP_099785196.1    Uniprot ID   -
Organism   Stenotrophomonas indicatrix strain D763     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3617780..3629507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KX724_RS16525 (KX724_16525) sucC 3617790..3618959 (-) 1170 WP_025874298.1 ADP-forming succinate--CoA ligase subunit beta -
  KX724_RS16530 (KX724_16530) - 3619336..3620949 (+) 1614 WP_218787887.1 ATP-binding protein -
  KX724_RS16535 (KX724_16535) pilR 3621004..3622377 (+) 1374 WP_025874296.1 sigma-54 dependent transcriptional regulator Regulator
  KX724_RS16540 (KX724_16540) pilB 3622780..3624507 (-) 1728 WP_099785196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KX724_RS16545 (KX724_16545) - 3624728..3625198 (-) 471 WP_306431525.1 pilin -
  KX724_RS16550 (KX724_16550) pilA/pilAI 3625250..3625669 (-) 420 WP_059064909.1 pilin Machinery gene
  KX724_RS16555 (KX724_16555) pilC 3626024..3627283 (+) 1260 WP_059064911.1 type II secretion system F family protein Machinery gene
  KX724_RS16560 (KX724_16560) - 3627291..3628154 (+) 864 WP_025874291.1 A24 family peptidase -
  KX724_RS16565 (KX724_16565) coaE 3628166..3628777 (+) 612 WP_032975178.1 dephospho-CoA kinase -
  KX724_RS16570 (KX724_16570) - 3628830..3629216 (-) 387 WP_218787888.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62279.22 Da        Isoelectric Point: 5.8722

>NTDB_id=587806 KX724_RS16540 WP_099785196.1 3622780..3624507(-) (pilB) [Stenotrophomonas indicatrix strain D763]
MNAVTTANLVGITGLARRLVQDGALDEVAARDAMAKAAAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDSS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DDGMANLDVGGGDDDMASTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPNKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPANLPEHA
LLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=587806 KX724_RS16540 WP_099785196.1 3622780..3624507(-) (pilB) [Stenotrophomonas indicatrix strain D763]
ATGAACGCCGTTACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGCCGCCTCGTCCAGGACGGCGCGCTGGACGA
AGTCGCTGCCCGCGATGCCATGGCCAAGGCAGCCGCCGCTCGTCAGCCCCTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTCGCTGCCGCCAACGCGGTCGAATTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACAGCAGC
CAGAACGCGATGAGCCTGGTCAGCGAAGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTCGGTACCAGCAACCCCACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTTGAGCCGATCCTGG
TGGACGAGGACCAGATTCGCCGCACGTTGGAGCAGTGGCATGCCAGCCATGACACGATCGGCGATGCGCTGGGTGGCGAC
GATGACGGCATGGCCAACCTCGATGTTGGCGGTGGCGACGACGACATGGCCAGCACCGACAGCGGTATCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGATTGACGCGATCCGCAAGGGCGCGTCGGACATCCATTTCG
AGCCCTACGAAGACGACTACCGCGTGCGTCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGAACAAGCTC
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCAT
CAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGCGTCAGCACGCTGCCTACGCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAGGCGATCCACAAGCCTTACGGCATGGTGCTGGTGACCGGCCCGACGGGTTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCGGAAGATCCTGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTGGGCGAAATCCGCGATCTGGAAACAGCGGAAATTGCGATCAAGGCTGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATTGCCCAGCGCTTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGCCCGGCCAATCTGCCGGAGCATGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAACTGTATGAGGCGGTGGGCTGCGACGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGCCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAATGCGCTGCAGATTGCCGAGGCTGCGCAGGCGATCGGTGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCC
GCGGGCGTGACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.575

100

0.563
  pilB Acinetobacter baumannii D1279779

56.437

98.609

0.557
  pilB Legionella pneumophila strain ERS1305867

51.937

98.783

0.513
  pilF Neisseria gonorrhoeae MS11

49.296

98.783

0.487
  pilB Vibrio cholerae strain A1552

49.037

99.304

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

99.304

0.456
  pilB Vibrio campbellii strain DS40M4

44.58

99.478

0.443