Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KUD94_RS01330 Genome accession   NZ_CP078069
Coordinates   283977..285710 (-) Length   577 a.a.
NCBI ID   WP_218238124.1    Uniprot ID   -
Organism   Comamonas sp. NLF-1-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278977..290710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUD94_RS01300 (KUD94_01300) - 279258..279821 (-) 564 WP_218238118.1 DUF6622 family protein -
  KUD94_RS01305 (KUD94_01305) - 279965..280252 (-) 288 WP_218238119.1 DUF1330 domain-containing protein -
  KUD94_RS01310 (KUD94_01310) zapD 280424..281179 (-) 756 WP_218238120.1 cell division protein ZapD -
  KUD94_RS01315 (KUD94_01315) coaE 281209..281826 (-) 618 WP_218238121.1 dephospho-CoA kinase -
  KUD94_RS01320 (KUD94_01320) pilD 281833..282729 (-) 897 WP_218238122.1 A24 family peptidase Machinery gene
  KUD94_RS01325 (KUD94_01325) pilC 282733..283950 (-) 1218 WP_218238123.1 type II secretion system F family protein Machinery gene
  KUD94_RS01330 (KUD94_01330) pilB 283977..285710 (-) 1734 WP_218238124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KUD94_RS01335 (KUD94_01335) rpmG 285898..286068 (-) 171 WP_146911641.1 50S ribosomal protein L33 -
  KUD94_RS01340 (KUD94_01340) rpmB 286088..286321 (-) 234 WP_146911640.1 50S ribosomal protein L28 -
  KUD94_RS01345 (KUD94_01345) - 286591..288354 (+) 1764 WP_218238125.1 methyl-accepting chemotaxis protein -
  KUD94_RS01350 (KUD94_01350) - 288440..288802 (+) 363 WP_255568944.1 DsrE/DsrF/TusD sulfur relay family protein -
  KUD94_RS01355 (KUD94_01355) - 288848..289306 (-) 459 WP_218238127.1 RidA family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62496.44 Da        Isoelectric Point: 5.1502

>NTDB_id=586537 KUD94_RS01330 WP_218238124.1 283977..285710(-) (pilB) [Comamonas sp. NLF-1-9]
MATAGTAQKEAAAVALPSLARALMSAGKLAQNSAEDISRKAQASRNSFIAELLAAKTLSAGDLAQTLSNTFGAPLLDVDA
LDAAHLPRDLLDAKLCQAYKVVALAKRSNRLIALTADPTNQEAAEKIKFTTQLGVDWVVGEYDKLLRLVEATTKSVSESL
DSITGGDFEFDDGPVEEEAEAPDTTGADVEDAPVVRFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRVDGELQEITSPPV
AIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEEKER
LLAAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFAVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPADIPH
ESLVEAGFREEDIDGSWVTYKPVGCSACNNGYKGRVGIYEVMPISEELQRIILADGSALEIAAQARAEGVRSLRQSGLHK
AKAGFTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=586537 KUD94_RS01330 WP_218238124.1 283977..285710(-) (pilB) [Comamonas sp. NLF-1-9]
ATGGCCACCGCCGGTACCGCCCAGAAAGAAGCAGCAGCCGTGGCATTGCCCAGCCTGGCGCGTGCGCTCATGTCGGCAGG
CAAGCTGGCGCAAAACAGCGCCGAAGACATCTCGCGCAAGGCGCAGGCCAGCCGCAACAGCTTCATTGCCGAGCTGCTCG
CCGCCAAGACCCTGAGCGCCGGTGATCTGGCGCAGACGCTGTCCAACACCTTTGGCGCACCGCTGCTCGACGTGGACGCG
CTGGACGCCGCCCACCTGCCCCGGGACCTGCTCGACGCCAAGCTCTGCCAGGCCTACAAGGTCGTCGCCCTGGCCAAGCG
CAGCAACCGGCTGATCGCACTCACCGCCGACCCGACCAACCAGGAAGCCGCGGAAAAGATCAAGTTCACCACCCAGCTCG
GCGTGGATTGGGTGGTCGGCGAATACGACAAGCTGCTGCGGCTGGTCGAGGCGACGACCAAGAGCGTCTCCGAGAGCCTG
GACAGCATCACCGGCGGCGACTTCGAGTTCGACGACGGACCGGTCGAGGAAGAAGCCGAAGCGCCGGACACCACAGGCGC
CGACGTCGAAGACGCGCCGGTCGTGCGTTTTCTGCACAAGATGCTGCTGGACGCATTCAACATGCGTGCCTCCGACCTGC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGCTTTCGCGTCGACGGCGAGTTGCAGGAGATCACCTCTCCGCCGGTG
GCCATCAAGGACAAGCTCGCCTCGCGCATCAAGGTGATCTCGCGCCTCGATATCTCGGAAAAGCGCGTGCCCCAGGACGG
GCGCATGAAGCTCAAGGTCGGGCCCGACCGGGTCATCGACTTTCGCGTCAGCACCCTGCCCACGTTGTTCGGCGAAAAAA
TCGTCATCCGCATTCTGGATCCGAGCAGCGCGCGCATGGGCATCGACGCTCTCGGCTACGAACCCGAAGAAAAAGAGCGC
CTGCTGGCCGCGATCAACCGCCCCTACGGCATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTCTA
CACCTGCCTGAACCTGCTGAACAAGCCGGGCGTGAACATCTCCACCGCCGAAGACCCTTCGGAAATCAACATGCCAGGGG
TCAACCAGGTCAACGTCAACGAAAAGGCGGGCCTGACCTTTGCCGTCGCGCTCAAGGCCTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACCTGGTGCT
CTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGGATTGCGCCGTTCAACATCGCCT
CCAGCGTGATTCTCATCACCGCGCAGCGCTTGGCCAGGCGCCTGTGCCCGCAATGCAAGCAGCCGGCCGACATTCCGCAC
GAAAGTCTGGTCGAGGCAGGTTTTCGCGAGGAAGACATCGACGGCAGTTGGGTCACCTACAAGCCGGTGGGCTGCTCGGC
CTGCAACAACGGCTACAAGGGACGCGTCGGCATCTACGAAGTGATGCCGATCTCCGAAGAGCTGCAACGCATCATCCTGG
CCGACGGCAGCGCACTCGAGATCGCCGCCCAGGCGCGTGCCGAAGGCGTGCGTTCGCTGCGCCAGTCGGGCCTGCACAAG
GCGAAGGCGGGCTTCACCTCGCTCGAAGAGGTGCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.044

97.92

0.539
  pilB Acinetobacter baumannii D1279779

55.963

94.454

0.529
  pilF Neisseria gonorrhoeae MS11

52.113

98.44

0.513
  pilB Legionella pneumophila strain ERS1305867

49.823

98.094

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

46.996

98.094

0.461
  pilB Vibrio campbellii strain DS40M4

46.466

98.094

0.456
  pilB Vibrio cholerae strain A1552

45.709

98.96

0.452
  pilF Thermus thermophilus HB27

39.531

96.014

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.824

92.548

0.378