Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EVX74_RS07675 Genome accession   NZ_CP078045
Coordinates   1586082..1587281 (+) Length   399 a.a.
NCBI ID   WP_004731151.1    Uniprot ID   A0A4V1VP90
Organism   Acinetobacter lwoffii strain AL_065     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1577784..1588331 1586082..1587281 within 0


Gene organization within MGE regions


Location: 1577784..1588331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVX74_RS07650 (EVX74_007610) - 1578893..1579942 (+) 1050 WP_004731158.1 3-deoxy-7-phosphoheptulonate synthase -
  EVX74_RS07655 (EVX74_007615) - 1580561..1581904 (-) 1344 Protein_1476 ISNCY family transposase -
  EVX74_RS07660 (EVX74_007620) - 1582052..1583377 (-) 1326 WP_129718006.1 ISNCY family transposase -
  EVX74_RS07665 (EVX74_007625) - 1583544..1584878 (-) 1335 WP_337971659.1 ISNCY family transposase -
  EVX74_RS07670 (EVX74_007630) - 1585075..1585503 (+) 429 WP_000877730.1 hypothetical protein -
  EVX74_RS07675 (EVX74_007635) pilU 1586082..1587281 (+) 1200 WP_004731151.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 45064.53 Da        Isoelectric Point: 6.3929

>NTDB_id=586510 EVX74_RS07675 WP_004731151.1 1586082..1587281(+) (pilU) [Acinetobacter lwoffii strain AL_065]
MFTAELLDEAEKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAINVPGVSRFRVNVFVQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGAISYEEALRNADSTNELRLQIKLKSNRQDSTAPASEATQFTMMQDPKADEKEAENTSVSE

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=586510 EVX74_RS07675 WP_004731151.1 1586082..1587281(+) (pilU) [Acinetobacter lwoffii strain AL_065]
ATGTTCACGGCAGAATTGTTGGACGAAGCAGAAAAATTCATGCATCACATGCTGACCAAGGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCGAGTATTAAACATCAAGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CAGCTGAAAAAACCAAGCTGTTTGCCTACAGCCTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTTGAGTGC
AATTTTGCCATCAATGTGCCGGGCGTTTCCCGTTTCCGGGTAAATGTGTTTGTGCAACAACTTAATGTCGGTATGGTAAT
TCGTACCATTACTGCAGAAATTCCAAATTTCCAGAAGCTGAAATTACCGGAATCGCTGAAACATGTGATTATGGAAAAAC
GTGGACTGGTACTGGTAGTCGGAGGAACGGGTTCAGGTAAATCAACTTCACTCGCAGCCATGATTGACTACCGAAATGAA
AATTCAGCAGGGCATATCATTACCGTAGAAGATCCGGTTGAATATGTGCATAAACATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCTTGGCATAATGCACTGAAAAATACCTTGCGTCAGGCTCCGGATGTAATTCTGATCG
GGGAAATTCGCGATACTGAAACTATGGAACATGCGATTGCTTTTGCGGAAACTGGCCATTTGTGTTTGGGAACTCTGCAT
TCCAATAATGCCAACCAGACTTTGGATCGGATTATCAACTTCTTCCCTGAAGAACGCCGGAATCAGTTATTGATGGATTT
GTCTTCAAATATGAAGGCAATTATTTCACAGCGTCTGATTCGGACTCAAGATGGTAAAGGCCGTCGGGCAGCGATTGAAA
TCATGCTGAATACGCCTTTGATTTCCGACCTGATCCTGAAAGGCGAGTTTCATGAGCTGAAAGCCATTATGGCTAAATCC
CGTGAACTGGGCATGCAGACTTTTGACCAAGCCTTATTTGATCTGTATAACCAAGGCGCCATTTCCTATGAAGAAGCTTT
GCGTAATGCCGATTCTACCAATGAGTTACGCCTGCAGATCAAGCTGAAAAGTAATCGACAGGACAGTACTGCACCAGCTA
GTGAAGCAACCCAATTCACCATGATGCAAGATCCAAAAGCGGATGAGAAAGAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V1VP90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.207

87.218

0.534
  pilU Acinetobacter baylyi ADP1

56.196

86.967

0.489
  pilU Vibrio cholerae strain A1552

51.913

91.729

0.476
  pilT Pseudomonas aeruginosa PAK

44.479

81.704

0.363
  pilT Acinetobacter nosocomialis M2

43.636

82.707

0.361
  pilT Acinetobacter baumannii D1279779

43.636

82.707

0.361
  pilT Acinetobacter baumannii strain A118

43.636

82.707

0.361