Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EVX74_RS01935 Genome accession   NZ_CP078045
Coordinates   446537..448246 (-) Length   569 a.a.
NCBI ID   WP_005265509.1    Uniprot ID   A0AAJ3AJA3
Organism   Acinetobacter lwoffii strain AL_065     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441537..453246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVX74_RS01905 (EVX74_001895) - 441656..441979 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  EVX74_RS01910 (EVX74_001900) rlmB 442114..442863 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EVX74_RS01915 (EVX74_001905) - 442870..443793 (+) 924 WP_004644926.1 DMT family transporter -
  EVX74_RS01920 (EVX74_001910) coaE 443815..444414 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  EVX74_RS01925 (EVX74_001915) pilD 444416..445276 (-) 861 WP_004281094.1 prepilin peptidase Machinery gene
  EVX74_RS01930 (EVX74_001920) pilC 445276..446505 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  EVX74_RS01935 (EVX74_001925) pilB 446537..448246 (-) 1710 WP_005265509.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EVX74_RS01940 (EVX74_001930) tpiA 448524..449315 (+) 792 WP_004281100.1 triose-phosphate isomerase -
  EVX74_RS01945 (EVX74_001935) secG 449329..449658 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -
  EVX74_RS01970 (EVX74_001960) rimP 450460..450984 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  EVX74_RS01975 (EVX74_001965) nusA 451019..452503 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63514.59 Da        Isoelectric Point: 4.7884

>NTDB_id=586498 EVX74_RS01935 WP_005265509.1 446537..448246(-) (pilB) [Acinetobacter lwoffii strain AL_065]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFRNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGTDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=586498 EVX74_RS01935 WP_005265509.1 446537..448246(-) (pilB) [Acinetobacter lwoffii strain AL_065]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCATTAGCGCATGCCAAACAAGAAAAAATCGATATTGTGGCTGAGTTAATTAACCAGCAGCAACTTTCTC
CAACCGTCATTGCTGAAACGATTTCAGTCGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCTCAAATT
CTTCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATTCTGCCGATTTTTAGAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCGACCAATATCGAAGCGATTGATGCAGTCCGCTTTTCCACCAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGTACCTTTGAATTCGATGAAGACTTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTACTAATTGATGCGATCCGTATGGGGGCTTCAGACTTACATTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTAGATGGGGTATTACGCCAGATTGCAACACCACCACTACAGCTGGCAAATCGCTTGTCTTCGCGT
CTTAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGAATTCTGGATCCCTCCA
GCGCGATGTTGGGAACTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGCATTAATCAAGTCAACGTCAACAATAAAG
TTGGTCTTACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCGTAATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACCTTGCATACCAATAGTGCGCC
TGAGACCTTGACCCGTTTACGAAACATGGGGGTGCCCTCTTTCAATATTGCCACTTCAGTAAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGTTTGTGTTCACAATGCAAGAAACCTGCAGATATTCCGCAACAAAGCTTACTGGAGATGGGCTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CAGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.175

100

0.803
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

49.195

98.243

0.483
  pilF Neisseria gonorrhoeae MS11

48.485

98.594

0.478
  pilB Vibrio campbellii strain DS40M4

48.566

98.067

0.476
  pilB Vibrio cholerae strain A1552

50.288

91.564

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.237

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.932

100

0.373
  pilF Thermus thermophilus HB27

36.991

99.297

0.367