NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	EVX74_RS01925	Genome accession	NZ_CP078045
Coordinates	444416..445276 (-)	Length	286 a.a.
NCBI ID	WP_004281094.1	Uniprot ID	N9QGW2
Organism	Acinetobacter lwoffii strain AL_065
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 439416..450276

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EVX74_RS01900 (EVX74_001890)	recN	439552..441213 (-)	1662	WP_121979630.1	DNA repair protein RecN	-
EVX74_RS01905 (EVX74_001895)	-	441656..441979 (+)	324	WP_004281089.1	pyrimidine/purine nucleoside phosphorylase	-
EVX74_RS01910 (EVX74_001900)	rlmB	442114..442863 (+)	750	WP_004281091.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
EVX74_RS01915 (EVX74_001905)	-	442870..443793 (+)	924	WP_004644926.1	DMT family transporter	-
EVX74_RS01920 (EVX74_001910)	coaE	443815..444414 (-)	600	WP_004281093.1	dephospho-CoA kinase	-
EVX74_RS01925 (EVX74_001915)	pilD	444416..445276 (-)	861	WP_004281094.1	prepilin peptidase	Machinery gene
EVX74_RS01930 (EVX74_001920)	pilC	445276..446505 (-)	1230	WP_004281096.1	type II secretion system F family protein	Machinery gene
EVX74_RS01935 (EVX74_001925)	pilB	446537..448246 (-)	1710	WP_005265509.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EVX74_RS01940 (EVX74_001930)	tpiA	448524..449315 (+)	792	WP_004281100.1	triose-phosphate isomerase	-
EVX74_RS01945 (EVX74_001935)	secG	449329..449658 (+)	330	WP_004281101.1	preprotein translocase subunit SecG	-

Sequence

Protein

Download Length: 286 a.a. Molecular weight: 32010.35 Da Isoelectric Point: 8.0624

                                >NTDB_id=586496 EVX74_RS01925 WP_004281094.1 444416..445276(-) (pilD) [Acinetobacter lwoffii strain AL_065]

MHDFLQYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMFLHPEQPVIDETKLSLSKPASACPKCHQP

IRWYQNIPVISWLVLRGKCSNCQNPISIRYPLVEILTAVCALLVVAVFGPTLQMLFGLILTYVLIALTFIDFDTQLLPDR

YTLPLAALGLGVNSYALYTLPGAAIWGYIIGFLCLWVVYYAFKIVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL

VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide

Download Length: 861 bp

                                >NTDB_id=586496 EVX74_RS01925 WP_004281094.1 444416..445276(-) (pilD) [Acinetobacter lwoffii strain AL_065]

ATGCACGATTTTCTTCAATATTTTATTCAAAACCCAACGGCGTTGTATATCGCAGTTGGGCTTTTTAGTTTATGTATCGG

CAGCTTTCTGAATGTCGTGATTTACCGCACTCCCAAGATGATGGAACAGGAATGGCGTACTGATTGCCAGATGTTCCTGC

ATCCGGAACAGCCGGTGATTGATGAAACTAAGTTAAGTTTAAGCAAACCCGCTTCAGCTTGCCCCAAGTGTCATCAGCCA

ATCCGCTGGTACCAGAATATTCCCGTCATTAGCTGGCTGGTCCTGCGCGGAAAATGTAGCAACTGCCAGAATCCGATCAG

CATCCGTTATCCTTTGGTTGAAATTCTGACGGCAGTTTGTGCACTCCTTGTCGTAGCAGTATTTGGCCCTACCCTACAAA

TGCTGTTTGGCCTGATCCTGACTTATGTGCTGATCGCACTCACTTTTATTGATTTTGACACCCAGTTACTGCCTGACCGT

TATACTTTACCCTTGGCCGCGCTAGGTTTGGGGGTAAATAGTTATGCACTTTATACCTTACCCGGTGCTGCCATCTGGGG

CTATATTATTGGTTTCCTGTGTCTGTGGGTGGTGTATTATGCATTTAAAATCGTTACCGGCAAGGAAGGTATGGGCTATG

GCGATTTTAAATTGCTTGCCGCCCTGGGTGCCTGGATGGGGCCTTTATTACTGCCTTTAATTGTGCTGCTGTCTTCTCTG

GTTGGTGCGATTATCGGGATTATCCTACTGAAAGTCCGCAAGGAAAATCAGCCTTTTGCCTTTGGTCCCTATATCGCGAT

TGCCGGCTGGATTGCTTTTTTGTGGGGCGAGCAGATTATGAAAGTGTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	N9QGW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter nosocomialis M2	80	99.65	0.797
pilD	Acinetobacter baumannii D1279779	79.649	99.65	0.794
pilD	Vibrio cholerae strain A1552	51.237	98.951	0.507
pilD	Vibrio campbellii strain DS40M4	49.826	100	0.5
pilD	Neisseria gonorrhoeae MS11	46.909	96.154	0.451

Detailed information NTG0586496