Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EGT68_RS13370 Genome accession   NZ_CP078042
Coordinates   2805779..2807503 (+) Length   574 a.a.
NCBI ID   WP_005403575.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_068     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2800779..2812503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT68_RS13335 (EGT68_013335) nusA 2801631..2803115 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  EGT68_RS13340 (EGT68_013340) rimP 2803153..2803677 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  EGT68_RS13360 (EGT68_013360) secG 2804300..2804629 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  EGT68_RS13365 (EGT68_013365) tpiA 2804642..2805433 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  EGT68_RS13370 (EGT68_013370) pilB 2805779..2807503 (+) 1725 WP_005403575.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGT68_RS13375 (EGT68_013375) pilC 2807528..2808757 (+) 1230 WP_125272053.1 type II secretion system F family protein Machinery gene
  EGT68_RS13380 (EGT68_013380) pilD 2808770..2809630 (+) 861 WP_004958611.1 A24 family peptidase Machinery gene
  EGT68_RS13385 (EGT68_013385) coaE 2809632..2810231 (+) 600 WP_087539736.1 dephospho-CoA kinase -
  EGT68_RS13390 (EGT68_013390) - 2810228..2811139 (-) 912 WP_004958607.1 DMT family transporter -
  EGT68_RS13395 (EGT68_013395) rlmB 2811170..2811919 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EGT68_RS13400 (EGT68_013400) - 2812109..2812435 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63692.86 Da        Isoelectric Point: 5.0709

>NTDB_id=586479 EGT68_RS13370 WP_005403575.1 2805779..2807503(+) (pilB) [Acinetobacter junii strain AJ_068]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIEQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LEKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=586479 EGT68_RS13370 WP_005403575.1 2805779..2807503(+) (pilB) [Acinetobacter junii strain AJ_068]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTTATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATTAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAACACAATAAACTCGAAAAACTAATTGAACAGCAGTTTGGAGATAGTGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCCCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGCGCCTCTGACTTACATTTTGAACCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGAGTACTCAGACAAATTGCTAATCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGAAAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TCGTGATTGCTCAACGTTTAGCCCGCCGCCTATGCTCGGAATGTAAACAACCTATCGAGATTCCTAAGCAGAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCTAACCCAGACTTTCAAATTTTTCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCATTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.424

100

0.817
  pilB Acinetobacter baylyi ADP1

76.87

100

0.77
  pilB Legionella pneumophila strain ERS1305867

52.788

93.728

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.274

95.296

0.479
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

51.451

90.07

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.641

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.764

93.031

0.361