Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU516_RS11955 Genome accession   NZ_CP077852
Coordinates   2432729..2433226 (-) Length   165 a.a.
NCBI ID   WP_078098371.1    Uniprot ID   -
Organism   Staphylococcus aureus strain N6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2427729..2438226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU516_RS11930 (KU516_11865) gcvPB 2427891..2429363 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  KU516_RS11935 (KU516_11870) gcvPA 2429356..2430702 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU516_RS11940 (KU516_11875) gcvT 2430722..2431813 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KU516_RS11945 (KU516_11880) - 2431972..2432496 (-) 525 WP_001015120.1 shikimate kinase -
  KU516_RS11950 (KU516_11885) - 2432486..2432632 (-) 147 WP_001792109.1 hypothetical protein -
  KU516_RS11955 (KU516_11890) comGF 2432729..2433226 (-) 498 WP_078098371.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU516_RS11960 (KU516_11895) comGE 2433144..2433443 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  KU516_RS11965 (KU516_11900) comGD 2433430..2433876 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU516_RS11970 (KU516_11905) comGC 2433854..2434165 (-) 312 WP_031763468.1 competence type IV pilus major pilin ComGC Machinery gene
  KU516_RS11975 (KU516_11910) comGB 2434179..2435249 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU516_RS11980 (KU516_11915) comGA 2435221..2436195 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU516_RS11985 (KU516_11920) - 2436247..2436870 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU516_RS11990 (KU516_11925) - 2436867..2437196 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU516_RS11995 (KU516_11930) - 2437196..2438182 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19155.01 Da        Isoelectric Point: 10.4428

>NTDB_id=583736 KU516_RS11955 WP_078098371.1 2432729..2433226(-) (comGF) [Staphylococcus aureus strain N6]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEHKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=583736 KU516_RS11955 WP_078098371.1 2432729..2433226(-) (comGF) [Staphylococcus aureus strain N6]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAACACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

95.152

100

0.952