Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MW397_RS04135 Genome accession   NZ_CP095529
Coordinates   888888..889328 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain GN06156     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883888..894328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW397_RS04110 (MW397_04110) coaE 884378..884998 (-) 621 WP_029305702.1 dephospho-CoA kinase -
  MW397_RS04115 (MW397_04115) - 885023..885067 (+) 45 WP_120795372.1 protein YacM -
  MW397_RS04120 (MW397_04120) guaC 885223..886266 (+) 1044 WP_001217337.1 GMP reductase -
  MW397_RS04125 (MW397_04125) hofC 886301..887503 (-) 1203 WP_000157287.1 protein transport protein HofC -
  MW397_RS04130 (MW397_04130) pilB 887493..888878 (-) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  MW397_RS04135 (MW397_04135) pilA 888888..889328 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  MW397_RS04140 (MW397_04140) nadC 889531..890424 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MW397_RS04145 (MW397_04145) ampD 890512..891063 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MW397_RS04150 (MW397_04150) ampE 891060..891914 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MW397_RS04155 (MW397_04155) aroP 891957..893330 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=583737 MW397_RS04135 WP_000360904.1 888888..889328(-) (pilA) [Escherichia coli strain GN06156]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=583737 MW397_RS04135 WP_000360904.1 888888..889328(-) (pilA) [Escherichia coli strain GN06156]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363