Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L30_RS09085 Genome accession   NZ_CP077365
Coordinates   1934184..1935896 (+) Length   570 a.a.
NCBI ID   WP_198071715.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain FDAARGOS 1400     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1929184..1940896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L30_RS09050 (I6L30_09050) nusA 1930074..1931558 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  I6L30_RS09055 (I6L30_09055) rimP 1931596..1932120 (-) 525 WP_216985756.1 ribosome maturation factor RimP -
  I6L30_RS09075 (I6L30_09075) secG 1932758..1933087 (-) 330 WP_174722721.1 preprotein translocase subunit SecG -
  I6L30_RS09080 (I6L30_09080) tpiA 1933100..1933894 (-) 795 WP_216985757.1 triose-phosphate isomerase -
  I6L30_RS09085 (I6L30_09085) pilB 1934184..1935896 (+) 1713 WP_198071715.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6L30_RS09090 (I6L30_09090) pilC 1935924..1937150 (+) 1227 WP_086496648.1 type II secretion system F family protein Machinery gene
  I6L30_RS19035 (I6L30_09095) pilD 1937150..1938010 (+) 861 WP_198071716.1 prepilin peptidase Machinery gene
  I6L30_RS09100 (I6L30_09100) coaE 1938012..1938608 (+) 597 WP_216985758.1 dephospho-CoA kinase -
  I6L30_RS09105 (I6L30_09105) - 1938611..1939519 (-) 909 WP_216985759.1 DMT family transporter -
  I6L30_RS09110 (I6L30_09110) rlmB 1939554..1940303 (-) 750 WP_019456777.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6L30_RS09115 (I6L30_09115) - 1940406..1940732 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63524.82 Da        Isoelectric Point: 5.1618

>NTDB_id=580922 I6L30_RS09085 WP_198071715.1 1934184..1935896(+) (pilB) [Acinetobacter seifertii strain FDAARGOS 1400]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=580922 I6L30_RS09085 WP_198071715.1 1934184..1935896(+) (pilB) [Acinetobacter seifertii strain FDAARGOS 1400]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGCGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAATCCGCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGATATTATTATGGTTGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTGGAAATGGGCTTT
ACCGAACAGGATCTAGCACATCCAGACTTTCGCGTTTTTCAACCTGTGGGTTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGTAATGCTCTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.018

100

0.97
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.515

92.632

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384