Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L29_RS14185 Genome accession   NZ_CP077238
Coordinates   3008901..3010613 (+) Length   570 a.a.
NCBI ID   WP_005079341.1    Uniprot ID   -
Organism   Acinetobacter pittii strain FDAARGOS 1399     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3003901..3015613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L29_RS14150 (I6L29_14150) nusA 3004789..3006273 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  I6L29_RS14155 (I6L29_14155) rimP 3006311..3006835 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  I6L29_RS14175 (I6L29_14175) secG 3007473..3007802 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  I6L29_RS14180 (I6L29_14180) tpiA 3007815..3008609 (-) 795 WP_005079338.1 triose-phosphate isomerase -
  I6L29_RS14185 (I6L29_14185) pilB 3008901..3010613 (+) 1713 WP_005079341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6L29_RS14190 (I6L29_14190) pilC 3010640..3011866 (+) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  I6L29_RS14195 (I6L29_14195) pilD 3011866..3012726 (+) 861 WP_005079345.1 prepilin peptidase Machinery gene
  I6L29_RS14200 (I6L29_14200) coaE 3012728..3013324 (+) 597 WP_005065869.1 dephospho-CoA kinase -
  I6L29_RS14205 (I6L29_14205) - 3013321..3014235 (-) 915 WP_005079347.1 DMT family transporter -
  I6L29_RS14210 (I6L29_14210) rlmB 3014271..3015020 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6L29_RS14215 (I6L29_14215) - 3015123..3015449 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63527.93 Da        Isoelectric Point: 5.0271

>NTDB_id=580176 I6L29_RS14185 WP_005079341.1 3008901..3010613(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1399]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKIVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=580176 I6L29_RS14185 WP_005079341.1 3008901..3010613(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1399]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAAATAGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCAATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGCAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGTGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGTGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTTAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCCGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.527

91.93

0.474
  pilB Vibrio campbellii strain DS40M4

50.575

91.579

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.655

98.596

0.361