Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU752_RS03040 Genome accession   NZ_CP077093
Coordinates   681253..683328 (+) Length   691 a.a.
NCBI ID   WP_186688849.1    Uniprot ID   A0A9E6PM66
Organism   Pseudomonas vanderleydeniana strain RW8P3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676253..688328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU752_RS03020 (HU752_003020) pilM 678467..679531 (+) 1065 WP_186688830.1 pilus assembly protein PilM Machinery gene
  HU752_RS03025 (HU752_003025) - 679531..680097 (+) 567 WP_186688832.1 PilN domain-containing protein -
  HU752_RS03030 (HU752_003030) pilO 680094..680717 (+) 624 WP_186688845.1 type 4a pilus biogenesis protein PilO -
  HU752_RS03035 (HU752_003035) pilP 680714..681241 (+) 528 WP_186688847.1 type 4a pilus biogenesis lipoprotein PilP -
  HU752_RS03040 (HU752_003040) pilQ 681253..683328 (+) 2076 WP_186688849.1 type IV pilus secretin PilQ family protein Machinery gene
  HU752_RS03045 (HU752_003045) aroK 683333..683851 (+) 519 WP_186688851.1 shikimate kinase AroK -
  HU752_RS03050 (HU752_003050) aroB 684001..685101 (+) 1101 WP_186688865.1 3-dehydroquinate synthase -
  HU752_RS03055 (HU752_003055) - 685112..686719 (+) 1608 WP_186688867.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74735.90 Da        Isoelectric Point: 5.4182

>NTDB_id=579132 HU752_RS03040 WP_186688849.1 681253..683328(+) (pilQ) [Pseudomonas vanderleydeniana strain RW8P3]
MNRILSSLGISLWMALVSPMVFAANLKTLDVAALPGDRVELKLAFDGAPPAPHGYTTEQPARIALDLTGVASQLATRTRD
LGVGNARSVTVVEAKDRTRLIINLTKLAPYNTRIDGNNLFVVVGQGAAPVASSQAASARPVAAAAQPARVAPKPYVPVGK
AIRSVDFQRGEQGEGNVVIELSDPSISPDIQEKGGKISVDFPRTQLPEPLRVRLDVKDFATPVQYVNATASADRASISIE
PGGAFDYSTYQTDNKLTISVRPLTVDDLQRRNAERFVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AESKGDERGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKQLGARWGGSISGGNWKTNG
GSPTLSKLTGQSSSTPFVDLGATSATSGIGIGFVTDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGTEIPYQ
ESSSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYQNVLNNVPPIKKNEVNAKVLVNDGETVVIGGVFSNT
QSKTEEKVPFLGDVPYVGRLFRRDIVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=579132 HU752_RS03040 WP_186688849.1 681253..683328(+) (pilQ) [Pseudomonas vanderleydeniana strain RW8P3]
ATGAACCGGATCCTTTCCTCCCTCGGCATTTCGCTATGGATGGCGCTGGTGTCGCCGATGGTGTTCGCGGCCAACCTCAA
GACCCTCGACGTGGCGGCTCTGCCAGGGGATCGGGTCGAGCTCAAGCTGGCCTTCGACGGCGCGCCGCCAGCCCCGCACG
GCTACACCACCGAGCAGCCGGCGCGGATCGCCCTGGACCTGACCGGTGTGGCCAGCCAACTGGCCACGCGGACCCGCGAC
CTGGGCGTGGGTAATGCGCGCAGCGTGACCGTGGTCGAGGCCAAGGATCGTACCCGGCTGATCATCAACCTGACCAAGCT
GGCGCCCTACAACACCCGAATCGATGGCAACAACCTGTTCGTGGTGGTCGGCCAGGGGGCGGCGCCCGTTGCGTCGAGCC
AGGCTGCCAGCGCCCGTCCGGTGGCTGCGGCGGCACAGCCTGCGCGGGTTGCACCGAAACCCTACGTGCCGGTGGGCAAG
GCCATTCGCAGTGTCGACTTCCAGCGGGGCGAGCAGGGCGAGGGCAATGTGGTCATCGAACTCTCCGATCCGTCCATCAG
CCCTGATATCCAGGAAAAAGGCGGCAAGATCAGCGTGGATTTCCCTCGTACCCAGTTGCCTGAGCCGCTGCGCGTACGCC
TGGACGTGAAGGATTTCGCCACGCCGGTGCAGTATGTCAACGCCACGGCTTCCGCCGATCGGGCGAGCATTTCCATCGAG
CCGGGCGGTGCCTTCGACTATTCGACCTACCAGACCGACAACAAGCTGACCATCAGCGTCCGTCCGCTGACCGTCGACGA
CCTGCAGCGGCGCAATGCGGAGCGTTTCGTCTACACCGGTGAAAAGCTCTCGCTCAACTTCCAGGACATCGACGTCCGCT
CGGTGCTGCAACTGATCGCCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGGATCACCCTGCGC
CTGCAGAACGTGCCATGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAATGT
GCTGCTGGTGGCGCCGGCGGACGAGATCGCCGCGCGTGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAGCTGG
CACCGCTGCGGCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGAGCAAGGGTGACGAGCGCGGTTCGATCACCGTGGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGA
ACGTCTCGATGAGCTGCGGCGAATCGTGGCGCAGTTGGACATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTCG
AAGCCAACGTCGGCTATGACAAGCAGTTGGGCGCCCGTTGGGGCGGCAGCATCTCCGGTGGCAACTGGAAGACCAACGGC
GGCTCGCCGACGCTGTCCAAACTGACCGGCCAGAGCAGCAGCACACCGTTCGTCGACCTGGGTGCAACCTCGGCAACGTC
CGGCATCGGTATCGGCTTCGTCACCGACAACACCATCCTCGACCTGGAACTGAGCGCGATGGAGAAGACCGGCAACGGTG
AAGTCGTGTCGCAACCGAAGGTGGTGACTTCCGACAAGGAAACCGCGAAGATCCTCAAGGGTACGGAGATCCCGTACCAG
GAATCGAGTTCCAGCGGTGCCACCTCGGTCTCCTTCAAGGAGGCGTCGCTGTCTCTCGAGGTCACGCCGCAGATCACCCC
GGACAACCGGATCATCATGGAAGTGAAGGTCACCAAGGATGAGCCGGACTACCAGAACGTGCTCAACAACGTGCCGCCGA
TCAAGAAGAACGAAGTCAACGCCAAGGTGCTGGTCAACGACGGTGAAACCGTGGTGATCGGTGGCGTGTTCTCGAATACT
CAGAGCAAGACCGAAGAGAAGGTGCCATTTCTGGGCGATGTGCCGTATGTTGGGCGCCTTTTCCGTCGGGACATCGTCTC
GGAAGCAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Secretin and TonB N terminus short domain (STN)

(306-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.059

100

0.768
  comQ Acinetobacter baylyi ADP1

42.017

100

0.434
  pilQ Acinetobacter baumannii D1279779

39.018

100

0.414
  pilQ Acinetobacter baumannii strain A118

39.018

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.166

97.829

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.829

0.373