Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HU752_RS03020 Genome accession   NZ_CP077093
Coordinates   678467..679531 (+) Length   354 a.a.
NCBI ID   WP_186688830.1    Uniprot ID   A0A9E6PMH3
Organism   Pseudomonas vanderleydeniana strain RW8P3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673467..684531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU752_RS03010 (HU752_003010) - 673789..675057 (+) 1269 WP_186687223.1 malic enzyme-like NAD(P)-binding protein -
  HU752_RS03015 (HU752_003015) - 675828..678263 (-) 2436 WP_186688829.1 penicillin-binding protein 1A -
  HU752_RS03020 (HU752_003020) pilM 678467..679531 (+) 1065 WP_186688830.1 pilus assembly protein PilM Machinery gene
  HU752_RS03025 (HU752_003025) - 679531..680097 (+) 567 WP_186688832.1 PilN domain-containing protein -
  HU752_RS03030 (HU752_003030) pilO 680094..680717 (+) 624 WP_186688845.1 type 4a pilus biogenesis protein PilO -
  HU752_RS03035 (HU752_003035) pilP 680714..681241 (+) 528 WP_186688847.1 type 4a pilus biogenesis lipoprotein PilP -
  HU752_RS03040 (HU752_003040) pilQ 681253..683328 (+) 2076 WP_186688849.1 type IV pilus secretin PilQ family protein Machinery gene
  HU752_RS03045 (HU752_003045) aroK 683333..683851 (+) 519 WP_186688851.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38183.55 Da        Isoelectric Point: 4.5919

>NTDB_id=579131 HU752_RS03020 WP_186688830.1 678467..679531(+) (pilM) [Pseudomonas vanderleydeniana strain RW8P3]
MLGLFGKKAHSLLGIDISSTSVKLLELSRSGNRYRVESYAVEPLPPNAVVEKNIAELEGVGQALTRVLLKARTSAREAAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALGL
AGLDARVVDVEAYALERSFGLLASQLAIDHDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVVEVLQPFKEALVQQVSRSLQFFFASGQYNDVDYILLAGGTASIAGLDHLIQQRLGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=579131 HU752_RS03020 WP_186688830.1 678467..679531(+) (pilM) [Pseudomonas vanderleydeniana strain RW8P3]
GTGCTTGGACTCTTCGGCAAAAAAGCGCATTCGCTCCTGGGGATAGACATCAGTTCGACCTCGGTCAAACTCCTCGAATT
GAGCCGCTCGGGCAATCGATATCGGGTCGAGTCCTATGCCGTGGAGCCGCTGCCGCCCAATGCCGTGGTCGAGAAGAACA
TTGCCGAGCTGGAGGGGGTCGGCCAGGCCCTCACCCGCGTGTTGCTCAAGGCGCGGACCAGTGCGCGCGAGGCCGCGGTG
GCCGTGGCCGGTTCCGCCGTCATCACCAAGACCATCGAGATGGATGCCGGGCTCTCCGACGACGACATGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCTATTCGGCGC
GCAACCCCGAGCGTGTCGAGGTACTGCTGGCCGCCTGTCGGAAAGAAAACGTCGAAGTGCGCGAGGCGGCGCTCGGCCTG
GCCGGGCTCGATGCCCGGGTGGTCGATGTCGAGGCCTATGCACTGGAGCGCTCCTTCGGCCTGCTGGCCTCCCAACTGGC
GATCGACCACGACAGGCTGACGGTCGCGGTGGTCGACATCGGTGCGACCATGACCACCCTCAGTGTCCTGCACAACGGGC
GGATCATCTATACCCGCGAGCAGTTGTTCGGCGGGCGCCAGCTCACCGAGGAAATCCAGCGCCGCTATGGCCTGTCCAAC
GAGGAGGCCGGCCTGGCGAAGAAGCAGGGTGGCCTGCCCGACGACTATGTCGTCGAGGTTCTGCAGCCGTTCAAGGAAGC
CCTGGTCCAACAGGTTTCGCGTTCCCTGCAGTTCTTTTTCGCCTCGGGCCAGTACAACGATGTCGACTACATCCTGCTGG
CTGGCGGTACCGCCTCGATTGCCGGGCTCGACCACCTGATCCAGCAGCGCCTGGGGACGCCGACCATGGTCGCCAACCCG
TTCGCCGACATGGCGTTGAGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCGCCGGCCCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

55.932

100

0.559
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463