Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU724_RS02910 Genome accession   NZ_CP077092
Coordinates   652197..654275 (+) Length   692 a.a.
NCBI ID   WP_137215141.1    Uniprot ID   -
Organism   Pseudomonas iranensis strain SWRI54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647197..659275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU724_RS02890 (HU724_002890) pilM 649412..650476 (+) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  HU724_RS02895 (HU724_002895) - 650476..651042 (+) 567 WP_056787334.1 PilN domain-containing protein -
  HU724_RS02900 (HU724_002900) pilO 651039..651662 (+) 624 WP_186567531.1 type 4a pilus biogenesis protein PilO -
  HU724_RS02905 (HU724_002905) - 651659..652183 (+) 525 WP_160768498.1 pilus assembly protein PilP -
  HU724_RS02910 (HU724_002910) pilQ 652197..654275 (+) 2079 WP_137215141.1 type IV pilus secretin PilQ Machinery gene
  HU724_RS02915 (HU724_002915) aroK 654280..654798 (+) 519 WP_024011270.1 shikimate kinase AroK -
  HU724_RS02920 (HU724_002920) aroB 654951..656051 (+) 1101 WP_186567529.1 3-dehydroquinate synthase -
  HU724_RS02925 (HU724_002925) - 656062..657648 (+) 1587 WP_186567527.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74935.18 Da        Isoelectric Point: 5.4106

>NTDB_id=579103 HU724_RS02910 WP_137215141.1 652197..654275(+) (pilQ) [Pseudomonas iranensis strain SWRI54]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDSPPPQPKGYTTESPARIALDLPGVTSQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=579103 HU724_RS02910 WP_137215141.1 652197..654275(+) (pilQ) [Pseudomonas iranensis strain SWRI54]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACAGCCCGCCACCCCAGCCCAAGG
GCTACACAACCGAGTCGCCGGCGCGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGTTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGTAACGCGCGTACCGCAACGGTGGCCGAAGCCAAGGATCGAACCCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGCAGCAGGGCGATTCGC
GGTGTCGATTTCCAGCGCGGCACCGAGGGTGAGGGCAACGTAGTCATCGACCTCTCTGATCCGACCATTGCCCCGGATAT
CCAGGAGCACGATGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACGTTTCAGACCGACAACAAGCTCACCGTCAGCGTGCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCTGACCGTCAGGCCTATGTCGGCGACAAGCTGTCACTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAACCTCAACCTCGTAGCAAGCGATACGGTGCAGGGCGGTATCACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGTTCGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAATAATAC
TTCGGGGATCGGCATCGCATTCATCACCGACAACGTGTTGCTGGATCTCGAACTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGTTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.207

100

0.747
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.517

100

0.402
  pilQ Acinetobacter baumannii D1279779

37.517

100

0.402
  pilQ Legionella pneumophila strain Lp02

37.83

98.555

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.832

0.37