Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HU724_RS02890 Genome accession   NZ_CP077092
Coordinates   649412..650476 (+) Length   354 a.a.
NCBI ID   WP_024011266.1    Uniprot ID   V8RDF4
Organism   Pseudomonas iranensis strain SWRI54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644412..655476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU724_RS02880 (HU724_002880) - 645160..646428 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  HU724_RS02885 (HU724_002885) - 646748..649195 (-) 2448 WP_186567533.1 penicillin-binding protein 1A -
  HU724_RS02890 (HU724_002890) pilM 649412..650476 (+) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  HU724_RS02895 (HU724_002895) - 650476..651042 (+) 567 WP_056787334.1 PilN domain-containing protein -
  HU724_RS02900 (HU724_002900) pilO 651039..651662 (+) 624 WP_186567531.1 type 4a pilus biogenesis protein PilO -
  HU724_RS02905 (HU724_002905) - 651659..652183 (+) 525 WP_160768498.1 pilus assembly protein PilP -
  HU724_RS02910 (HU724_002910) pilQ 652197..654275 (+) 2079 WP_137215141.1 type IV pilus secretin PilQ Machinery gene
  HU724_RS02915 (HU724_002915) aroK 654280..654798 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38045.57 Da        Isoelectric Point: 4.7539

>NTDB_id=579102 HU724_RS02890 WP_024011266.1 649412..650476(+) (pilM) [Pseudomonas iranensis strain SWRI54]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=579102 HU724_RS02890 WP_024011266.1 649412..650476(+) (pilM) [Pseudomonas iranensis strain SWRI54]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTGGAGCCATTGCCGGCAAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCGGCGGTGATCACCAAAGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAGAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCACAGGAGCGCCTGACCGTGGCGGTGGTCGACATTGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTTGGCGGGCGCCAACTCACCGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAGCAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CGGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGTCTGGATACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCCCTGGGCAGCAAGGTTAACGCCGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8RDF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483