Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU754_RS03155 Genome accession   NZ_CP077090
Coordinates   713098..715179 (+) Length   693 a.a.
NCBI ID   WP_186621291.1    Uniprot ID   A0A9E6NQG7
Organism   Pseudomonas zeae strain OE 48.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708098..720179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS03135 (HU754_003135) pilM 710310..711374 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  HU754_RS03140 (HU754_003140) - 711374..711940 (+) 567 WP_186621295.1 PilN domain-containing protein -
  HU754_RS03145 (HU754_003145) pilO 711937..712560 (+) 624 WP_099757776.1 type 4a pilus biogenesis protein PilO -
  HU754_RS03150 (HU754_003150) - 712557..713084 (+) 528 WP_186621293.1 pilus assembly protein PilP -
  HU754_RS03155 (HU754_003155) pilQ 713098..715179 (+) 2082 WP_186621291.1 type IV pilus secretin PilQ family protein Machinery gene
  HU754_RS03160 (HU754_003160) aroK 715184..715702 (+) 519 WP_011332081.1 shikimate kinase AroK -
  HU754_RS03165 (HU754_003165) aroB 715855..716955 (+) 1101 WP_186621289.1 3-dehydroquinate synthase -
  HU754_RS03170 (HU754_003170) - 716966..718555 (+) 1590 WP_186621287.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75123.62 Da        Isoelectric Point: 5.8300

>NTDB_id=579043 HU754_RS03155 WP_186621291.1 713098..715179(+) (pilQ) [Pseudomonas zeae strain OE 48.2]
MNRIFSTLGFSLWIALMSPMVLAANLRTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYTTRVEGNNLFVVVGQGAPATAPRPAAAAPRATAKAAAPAKAFVPKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGTSSTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=579043 HU754_RS03155 WP_186621291.1 713098..715179(+) (pilQ) [Pseudomonas zeae strain OE 48.2]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAG
GACGCTGGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGTTGAAGTTGTCGTTCGACGGCCCGCCACCACAACCCAAGG
GCTACACCACCGAATCGCCGGCGCGGATTGCGCTGGACCTGCCCGGTGTCGCCAGTCAGTTAGCCAGCAAGAACCTCGAT
CTGGGCAGCGGCAATGCGCGAACCGCTACTGTGGTTGAGGCCAAGGAACGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACACCACGCGGGTTGAGGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCGGCCACCGCGCCGCGTC
CCGCTGCCGCTGCACCTCGTGCTACCGCTAAAGCTGCTGCGCCCGCCAAGGCTTTTGTGCCGAAAAGCCGGGCGATTCGC
GGTGTGGACTTCCAGCGTGGTACGGCGGGGGAAGGCAATGTGGTCATCGACCTGTCCGACCCGACTATTGCTCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAGTTACCAGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTCGCCACTCCGGTGCAGTTCGTCAATGCCGGAGTAACCGCTGATCGCACAGTCATTACCGTTGAACCCAGTGGC
ACCTACGAGTACTCCACCTTCCAGACCGACAACAAACTGACCGTCAGCATCCGCCCGATGACTGTCGATGACCTGCAAAA
GCGTAACGCTGACCGCCAGGCGTACGTCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTTGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGCCTGCAAAAT
GTGCCGTGGGATCAGGCCCTGGATCTGGTGTTGAAAACCAAGGGCCTGGATAAGCGCAAGATCGGCAATGTGTTGCTAGT
GGCTCCGGCCGATGAAATCGCTGCCCGCGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GTCGTGAGTTGCTGCAAGTGAATTACGCCAAAGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCTGAGGCG
AAAATCGACGAGCGCGGTTCGATTACTGTCGACGAGCGGACCAACAACATCATTGCCTACCAGACTCAGGATCGCCTCGA
CGAACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGCGGATTGTCGAGGCCAACG
TCGATTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACCTCGGGCGTGAGC
AACGGCACATCGTCGACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTGGGCAA
TACCTCAGGGATCGGCATCGCCTTCATCACCGACAACGTGCTCCTCGATCTCGAGCTGACCGCGATGGAGAAGACCGGTA
ACGGCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCG
TATCAGGAAGCCAGTTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAAGCCTCGCTGTCACTGGAAGTCACCCCGCAGAT
CACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTATCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.361

100

0.759
  comQ Acinetobacter baylyi ADP1

41.354

100

0.414
  pilQ Acinetobacter baumannii D1279779

37.936

100

0.408
  pilQ Acinetobacter baumannii strain A118

37.936

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

37.87

97.547

0.369
  pilQ Legionella pneumophila strain Lp02

37.87

97.547

0.369